Tagged Questions
5
votes
2answers
47 views
Is wiping with RNAse Zap enough to destroy RNAse activity?
From the RNAseZap MSDS, it is an SDS at some unknown concentration, maybe with some NaOH?
Some other links suggest there is some NaOH as well.
The Ambion site states that RNAseZap destroys RNAse ...
1
vote
0answers
32 views
Can I heat Trizol?
I wonder if I can heat Trizol reagent for 30 min 65C. The goal is to disrupt protein-RNA complex while inhibiting nucleases. (I can't use RNasin cause it's inactivated in 65C, and can't use RVC cause ...
4
votes
1answer
72 views
Is DNA green viewer carcinogenic?
I use DNA green viewer in Lab to see DNA and RNA bands. Some peers told me it is carcinogenic and is not safe.
Is this correct? If yes, are there better choices to use in working with gel?
5
votes
1answer
84 views
What are good practices with reusing desalting columns
At least according to a few sources Prozyme and Protocols-Online, it is possible to reuse desalting columns and since I'm cheap I would like to also.
Key things seem to be washing with several column ...
