A genus of retroviruses with a lengthy incubation time, in particular HIV. Commonly used as DNA transduction agents in animal cells.

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lentivector security

Lentivectors are widely used in molecular biology, most commonly to transduce in a stable way a desired gene. These vector system takes advantage from the ability of viruses to introduce their own ...
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Should gene therapy safety protocol include isolation?

In the case of a gene therapy trial where viral vectors are used to deliver genes into mammalian cells, including humans, should biosafety and ethical protocols include isolation of the patient as a ...
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Are some virus loads introduced to human cell but never triggered?

Is there a term or any evidence of phage DNA integrating into chromosomes/ DNA but never being triggered? For example, could a virus that affected Neanderthals still infect human cells today but is ...
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Virus titre and copy number of integration

If I need to preform a genome-wide high throughput screen using pooled shRNA library infection followed by deconvolution by deep sequencing, how would I determine the optimal infection rate at which ...
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How to calculate virus titre from qPCR

I harvested some lentivirus from 293T cells and want to titre the result. I infected 293T cells on a well plate with 400,000 cells per well which I infected with virus stock, and 1 in 10, 100 and 1000 ...