I was working through a previous exam and came across the following question (with reference to the picture shown below): "What MS ionization method was employed (MALDI or ESI)?" I cannot seem ...
I've reading about proteomics and how it can be used with mass spectrometry to sequence digested peptides. Reading this paper the author states (in box 2): peptide fragmentation induced by ...
I began reading this paper (http://www.biomedcentral.com/1471-2105/14/56) and had a few questions about mass spectrometry terminology that I couldn't find answers to elsewhere. Consider the following ...
Which mass spectrometry database search algorithms include the fragment mass accuracy in the calculation of protein scores?
E.g. in a MASCOT search, the accuracy of the fragment ions in a ms/ms spectrum does not have any influence on the scores of identified proteins (unless the fragment ions are out of the set ms/ms ...
To detect the deamidation from Glutamine to Glutamate in a protein, normal practise seems to be a mass spec, where it is claimed there is a change of 1 dalton. EG It is claimed that: Deamidation ...