Once you have your gel with separated proteins can you simple cut out each band and plop it into solution or do you need to take extra steps?
I began reading this paper (http://www.biomedcentral.com/1471-2105/14/56) and had a few questions about mass spectrometry terminology that I couldn't find answers to elsewhere. Consider the following ...
I've reading about proteomics and how it can be used with mass spectrometry to sequence digested peptides. Reading this paper the author states (in box 2): peptide fragmentation induced by ...
I was working through a previous exam and came across the following question (with reference to the picture shown below): "What MS ionization method was employed (MALDI or ESI)?" I cannot seem ...
Which mass spectrometry database search algorithms include the fragment mass accuracy in the calculation of protein scores?
E.g. in a MASCOT search, the accuracy of the fragment ions in a ms/ms spectrum does not have any influence on the scores of identified proteins (unless the fragment ions are out of the set ms/ms ...
To detect the deamidation from Glutamine to Glutamate in a protein, normal practise seems to be a mass spec, where it is claimed there is a change of 1 dalton. EG It is claimed that: Deamidation ...