1
vote
3answers
85 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
1
vote
1answer
134 views

Why does beta-2-Thienylalanine inhibit bacterial growth?

I'm trying to obtain a better understanding of the Guthrie Test, which checks whether people have a disease called PKU. The Guthrie test uses bacteria to check for Phenylalanine (the amino acid) in ...
2
votes
1answer
72 views

Aerobe or facultative anaerobe organism that metabolizes acetate?

I'm assuming bacteria, but will take any suggestions. Organism must survive primarily on the acetate (plus trace elements), but I can give/take electrons, if necessary. Not interested in strict ...
5
votes
1answer
152 views

What effect do oligosaccharides, like those found in legumes, have on the composition of intestinal flora in humans and if so how so?

I know that intestinal flora metabolising oligosaccharides, like those found in legumes, is the cause for the well known fact that legumes cause flatulence, but does an oligosaccharide-rich intestine ...
0
votes
2answers
77 views

How might one determine if bacteria are nitrogen fixing or not?

If you had an isolate bacteria sample how would you determine if they were nitrogen fixing?