Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.
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Endophytic Xylariaceae: diversity and taxonomy inferred from rDNA sequence analyses
"Thailand is considered as one of the areas containing a high percentage of unknown taxa of Xylariaceae (Rogers 2000). In Thailand, several studies on endophytic fungi have been documented, namely, ...
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International standard to prepare an International journal [closed]
Any of you could give the link or reference regarding International standard to prepare an International journal on microbiology field?
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28 views
Wheat anthracnose outbreaks caused by Colletotrichum cereale in the USA
"Wheat anthracnose outbreaks caused by C. cereale were problematic during the 1940s in the USA, but presently do not limit production of this crop, despite the fact that the fungus still inhabits ...
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By what mechanism is Streptococcus bovis acting as a risk factor for colorectal cancer?
Streptococcus bovis bacteremia/endocarditis is considered a risk factor for colorectal cancer. What pathophysiological mechanism may link the two together?
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Writing a paper
Considering that I am writing a paper for a journal, could the following phrase be understood? or should I put the 'step-by-step' way by giving numbers?
PCR Amplification and sequencing
PCR ...
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44 views
“Oblong to Allantoids” … Is it valid?
I read on the text book:
The characteristic of Dinemasporium are: superficial, cupulate to Discoid conidiomata with brown satae, Phialid conidiogenous cells that give rise to hyaline, oblong to ...
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37 views
Is it possible for a person to become “reinfected” with the same strain of a virus?
If a person contracts a virus, viral conjunctivitis for example, is it possible for the individual to become "reinfected" with the exact same strain of the virus once the person has it treated and the ...
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38 views
Highly spherical Radiolaria; does this image accurate depict the organism?
If I understand correctly, Cenosphaera spp. is a type of Radiolaria, which is a type of zooplankton. The following is an image of Cenosphaera spp. is from the supplementary material (Figure S2) of ...
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Looking for detailed nutrient/energy flow at the bottom of the freshwater food chain
I am trying to model the production and consumption of nutrients and waste at the bottom of the food chain in freshwater ecosystems.
I can only find broad information on the Internet and don't know ...
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20 views
What is a good review book/source for microbiological diagnosis?
I am trying to find the diagnosis figure for different bacteria where phases are illustrated.
I am using at the moment: 1) First Aid for the USMLE Step 1 2013 and 2) Medical Microbiology by Murray et ...
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58 views
Lineage selection in plasmid evolution
I've been reading through Paulsson (2002) and I am not sure what he means by "lineage selection" in the second to last section. The paper deals with plasmid replication, and mostly concentrates on the ...
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How is the appropriately-stratified gut microbiome acquired in organisms performing horizontal transmission?
I am studying horizontal transmission of primary symbionts in insect reproduction. This reminded me of an earlier question I had asked, in which I learned that humans analogously acquired their ...
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36 views
What molecular processes are involved in pseudopodial extension?
I am curious as to the processes and mechanisms involved in the extension of pseudopodia in amoeba. How does the cell know and control the direction and extent of pseudopodia formation at a molecular ...
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57 views
How is the exogenous DNA protected from degradation during bacterial transformation?
During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
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22 views
Definition of Dye- Reduction Test?
Can some one give a simple explanation or definition on what a dye-reduction test is.
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1answer
18 views
Multiplication to formalize Microbiological Processes
I am not from the field, but have a question regarding modeling in biology:
I know that some processes can be modeled with the mathematical multiplication operation, and wondered if anyone could ...
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56 views
Where would Saccharomyces cerevisiae be found in highest concentrations in environment?
I have bakers asthma specifically to Sc yeast, and know to avoid bakeries, breweries, etc. Have figured out to not disturb rotting leaf piles, and fermenting bark mulch.
Where else.? What percentage ...
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96 views
Why are pili more common in Gram negative bacteria than in Gram positive?
Although pili have been observed in some species of Gram positive bacteria, the preliminary research that I have done indicates that pili are significantly more common in Gram negative bacteria.
Is ...
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2answers
123 views
Is every part of a virus important for replication?
Is every component of a virus absolutely essential for its infection and replication in a host cell? Or can you just have parts of it to cause infection?
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Can viral proteins cause infection? [duplicate]
Possible Duplicate:
HIV Rapid Tests
Can proteins found on the surface of RNA viruses cause infection without the presence of the actual RNA and other components used in the virus?
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42 views
Solid phase use in HIV rapid tests
I have another question in regards to my HIV test research. The rapid tests like Orasures Oraquick contains a strip of synthetic peptides that are used to represent proteins found in the envelope ...
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2answers
120 views
HIV Rapid Tests
The hiv antigens that are used in the oral rapid tests, are they infectious? The tests do not contain any actual virus but I am curious if the antigens themselves could somehow create the virus on ...
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1answer
88 views
If re-boiling does not curdle the milk, can that be taken as an indicator that the milk hasn't developed the bacteria (and their toxins)?
Usually when left in hot temperature, the milk curdles (which I know when I boil it and it starts separating).
If the re boiling does not curdle the milk, can that be taken as an indicator that the ...
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141 views
Bacterial cell lysis - what solution to use?
I am trying to determine how quickly detergents act on bacterial cells (cell lysis). I would like to compare some detergents at difference concentrations for bacteriolytic activity. I don’t care about ...
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2answers
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Are all known germs benign in small enough quantities? i.e. Why do we wash our hands?
We know that we wash our hands because germs can get into our body through the nose, the mouth, the eyes, cuts, etc. But I doubt we can completely clean our hands of germs every time we wash them, ...
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76 views
What types of archaea have been found in animals?
I am curious as to the species or "types" of archaea that have been found to reside within animals symbiotically. One of the only ones I can think of off the top of my head are methanogens, which live ...
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205 views
How much weight/volume do microbes occupy within the human body?
Microorganisms constitute the bulk of all the biomass on Earth. I weighed myself yesterday, and wondered how much less I would weigh if I were completely free of bacteria and microbes, inside and out. ...
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41 views
Colonial Cells Demonstrating Cell Specialization
Are there any living examples of cellular colonies demonstrating very primitive cellular specialization? If so, what do we know about how they assimilate? How independent are the individual cells ...
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Would two species of yeast with similar genome sizes have the same number of genes or chromosomes?
Similar organisms generally have similar genome sizes. Given this, would two species of yeast have the same number of genes and chromosomes?
Edit: Fixed with thanks to @daniel-standage
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How was the diversity between ethanol fermentation and lactic acid fermentation evolved?
Quite simply, some organisms metabolize glucose under anaerobic conditions via Glucose->(2) Pyruvic Acid->(2)Ethyl Alcohol. Some organisms, however, metabolize to lactic acid.
When did such a process ...
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67 views
Should we be looking for extra-terrestrial life on comets?
I have just read What elements are a possible basis for life? and I find myself wondering whether instead of seeking advanced life-forms at remote locales a La SETI, and perhaps fundamentally ...
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88 views
Can the RNA in the HIV virus make viral enzymes without entering the nucleus?
If the provirus was not formed yet, can the virus make viral enzymes?
(I know that it already has some, but supposing it doesn't)
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99 views
What does “cellular” mean in this context?
I came across a confusing word when I was reading a Scientific American story, “Controversial Spewed Iron Experiment Succeeds as Carbon Sink” (by David Biello). It goes like this:
“One key to the ...
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103 views
Can Naegleria fowleri enter through the eyes ( example rinsing/splashing eyes with water)
I understand that it primarily enters the body through the nose by by "feeding on the olfactory neurons in the nose" as answered here in a different question. Would the nerve cells in the eyes present ...
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113 views
“Acellular” designation for organisms
Why do some biologists refer to single-celled organisms such as Amoeba and Paramecium as
acellular (i.e., without cells) rather than unicellular (i.e., one cell)?
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281 views
Why does milk contained in cartons of milk expand?
In the morning, I went to the fridge to drink some chocolate milk. At night, when I took out the same carton of milk, the packaging seems expanded, like some kind of air is inside. Explain please !
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139 views
Sparking during electroporation of plasmid DNA into bacterial cells
During electroporation of bacterial cells (I work with Mycobacterium tuberculosis, but I think this applies to E. coli as well), sometimes I get sparking. I've read this is due to salts present, ...
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71 views
What is the effect of garlic on intestinal flora?
Now that we can compare whole microbiomes, has there been investigations on the effect of garlic (or diallyl disulfide, its main ingredient) on intestinal flora? From the proven antimicrobial ...
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Salvaging a plasmid from a cell culture stored at the incorrect temperature
I have E. coli with transformed plasmid on agar in a vial. It has been stored at -20 degree C without glycerol stock for 18 hours.
This is a continuation of: Survival of E. coli on agar plate at -20 ...
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161 views
Survival of E. coli on agar plate at -20 degree C without glycerol stock
If I kept E. coli transformed with plasmid at -20˚C without making glycerol stock, will it survive? Actually I have kept it this way for 18 hours. What will happen with it? Will it grow in fresh ...
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115 views
Models of quorum sensing for multi-agent systems
Quorum sensing is a system of stimulus and responses correlated to population density that is used by bacteria to coordinate gene-expression. I am looking for a simple computational/mathematical model ...
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74 views
Mutation-immunity in Luria-Delbruck experiment
If experiments like those of Luria and Delbruck on E. coli and T1 phage are the main source of our confidence in the mutation-immunity model, is it then highly unlikely that there are other types of ...
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1answer
120 views
Non-monotonic knock-out effects in prokaryotes
Typically, when performing gene-knockout, the experimenters select one gene to remove/replace-with-junk and then see if the prokaryote can still undergo fission. If it continues to reproduce then the ...
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2answers
165 views
Does making yogurt from non-pasteurized milk work against possible disease bacteria?
In the past, when there was no pasteurization, could making yogurt from milk lower the chance of getting infected by bovine tuberculosis (or other diseases from infected milk)?
For example, would ...
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2answers
163 views
Short-term Lamarckism in asexual single cell organisms
I was reading through the Karr et al. (2012) whole-cell computational model. One of the things they did was to induce single-gene disruptions in their model. They observed several to be fatal, but:
...
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157 views
Aren't antibiotic resistant probiotics dangerous?
Multidrug resistant probiotics are often recommended by doctors in various cases. But since bacteriae can easily exchange genes by conjugation or other means they could promote the drug resistance of ...
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28 views
Where can I find approximate rates of sequestration of CO2 for different species of algae?
For a study, I want to compare the rates of CO2 sequestration and fixation of a few different species of algae.
I could not find any data on the sequestration rates. Any pointers to where I can find ...
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322 views
Is there a free alternative to Gelcompar for comparing banding patterns across multiple gels?
In order to run my microbial community samples from my experiments through DGGE, I was required to use multiple gels.
Thus it is necessary to compare banding patterns across more than one gel. ...
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Vigorous shaking for HFR interrupted mating
I am trying to reproduce E. coli interrupted mating using an HFR strain, and I read that the cultures should be vigorously shaken at times to interrupt the DNA transfer from the F+(HFR) donors to the ...
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Is it plausible that eukaryotic organelles like flagella and cilia are the result of endosymbiosis with spirochetes?
This was a claim by Lynn Margulis explained over at this link.
The sense organs of vertebrates have modified cilia: The rods and cone
cells of the eye have cilia, and the balance organ in the ...
