Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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Is there any technique other than restriction enzymes to introduce staggered cuts into DNA?

I'm curious about introducing staggered cuts into DNA, but wonder if there is a way to do this in vivo in E. coli, as I heard introducing restriction enzymes on a plasmid would end up just shredding ...
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16 views

How do you technically perform the Ames test?

Is it necessary to take into account the volume of gm-agar(bottom) in the concentration determination, or is that determined only by the concentration in the top agar? I found several articles that ...
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Do disaccharidases require a cofactor or coenzyme to function?

I'm doing research on lactose intolerance and am curious if disaccharidases (enzymes that break down disaccharides) require a cofactor or coenzyme to function? Reviews or references would be greatly ...
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31 views

What is the meaning of a reported OD600 value?

I can't find a reference that explains what, for example, OD600 = 0.1 means. For example, Wikipedia states OD600 is an abbreviation indicating the absorbance, or optical density, of a sample ...
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32 views

Optical Density conversion to cfu / ml

I am having troubles understanding the correlation between OD measurements and CFU/ml. What is the conversion factor between the two units, and does this conversion factor somehow compensate for dead ...
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16 views

Does prokaryotic transcription have activators / enhancer regions involved?

I am designing a biosensor, and I need to know whether prokaryotic transcription involves or can involve (if a gene needs to be regulated) enhancer regions. Also, where are enhancer regions located (...
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1answer
30 views

What can saliva tell about ones health? [closed]

I was wondering if I d want to analyze something related to my current health based on my saliva. What could it be? I mean e.g. by putting a thermometer in your mouth you can know your body ...
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10 views

What membrane material(s) is/are used in a diffusion chamber and where can I get this/these material(s)?

I am participating in community biolab and am investigating using diffusion chambers for culturing novel species of bacteria as well other experiments. The type of diffusion chamber I am hoping to use ...
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1answer
26 views

Fine permanent fluorescent marker for blue light

I am looking for a fine- (or small-)tipped permanent marker to write on Petri dishes that is visible under a blue light or a transilluminator. It would seem obvious, but it is a nuisance. The ...
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15 views

Term of the type xxx-troph for a compound not used by an organism

A prototroph for compound X can make it, a bradytroph grows faster if X is scavenged, an auxotroph needs to scavenge it and a hyperauxotroph lacks both the biosynthetic pathway and the transporters. ...
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49 views

Geographical distribution of malaria and tuberculosis

Unlike malaria, tuberculosis (TB) is found across the whole world. Why and explain? I mean why are people affected with TB more than malaria and some say that in cold and developed countries malaria ...
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17 views

Did Cyanobacteria alone create the oxygen in Earth's atmosphere 3.5 billion years ago?

In other words, is the conveniently close-to-Californian-Universities pools of such representative of accepted theory. With the immediate follow-up question, when did the much more impressive amount ...
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19 views

How does the body know to stop effector cell proliferation?

After a pathogen has been successfully neutralized, effector cells such as the plasma cells and the cytotoxic T cells which are specific for that pathogen decrease in numbers. What is the signal in ...
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40 views

Link between NaCl and sulfide reducing bacteria

Could there be any link between the consumption of NaCl and sulfide-reducing bacteria? In my study on mice, sulfide-reducing bacteria (SRB) are more abundant, in high-salt-fed group compared to the ...
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45 views

Has Staphylococcus aureus ever been used against cancer?

Treating inoperable tumors with bacteria appears to be very effective, albeit being toxic to the patient as well. Has (live attenuated) Staphylococcus aureus ever been used against cancer? If the ...
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15 views

Red transcriptional reporter in Psudomonas putida

I need to build a transcriptional reporter in P. putida with high excitation wavelength,in order to decrease phototoxicity (I have been using mNeonGreen until now, and I would like to try something ...
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24 views

How do I “reinoculate” a petri dish with new bacteria?

Hypothetical question, intended to find a general procedure rather than case-specific recommendations: I have a petri dish full of growth medium with a large population of gram-negative, anaerobic ...
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37 views

Is diarrhoea advantageous to the microbe?

Diarrhoea is a common side effect of many feco-orally transmitted bacterial infections. How does diarrhoea help the pathogen? Should it not have a selective evolutionary advantage? Do all symptoms of ...
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46 views

Comments on Experimental result regarding Anti-Bacterial soap

After using soaps, we rinse our hands with water. Do bacteria from the water attach themselves to our hands again? If we wipe our hands to cloth after washing our hands, do bacteria from the cloth ...
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67 views

Why do gram-positive bacteria cause respiratory diseases more frequently than gram-negative bacteria? [closed]

My theory is that aerobic respiration by gram positive bacteria thrives in ideal conditions of the lungs therefore creates respiratory diseases?
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43 views

Is this bacteria or fungi?

I've been growing bacteria on an agar plate and after putting the dishes in the incubator, this is what I saw. I'm new to bacteria identification, and is this just contamination by fungi or is this ...
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51 views

What prevent us from synthesizing a cell from “scratch”?

In this recent article "Design and synthesis of a minimal bacterial genome", they created a minimal cell with only 473 genes. However, they didn't synthesize all of the necessary components of the ...
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171 views

Can viruses be toxic?

Bacteria can produce toxins like endotoxins and exotoxins. In diseases like cholera or tetanus they can harm infected people due to these toxins. Now, although viruses are much smaller and are ...
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Does alpha-amylase from different species have different *optimal* conditions?

Do the optimal conditions for the enzyme isoforms differ between species? Specifically, do the optimal pH and temperature for alpha-amylase differ for that enzyme produced by B. lichiniformis and A. ...
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45 views

What kind of organism grows in two-day-old lemon water? [closed]

I had a cup of lemon water on my desk that had been sitting out for 2-3 days. Today I took a sip without even realizing this. I looked at the water and it had something growing in it that looked like ...
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14 views

Growth charecteristics of Bacillus and Lactobacillus Species [closed]

Does anybody know weather the media (Hichrome Bacillus Agar) supports both Bacillus and Lactobacillus species, it is supposed to be the sample of the Lactobacillus plantarum spread on Hichrome ...
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46 views

What happens during the fermentation process of the eco-enzyme?

introduction about the eco-enzyme I have tried to make several ones at home, no matter what I am using, lemon or pineapple peels with brown or white sugar, the final products all show the similar ...
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21 views

Do plant and animal bacterial pathogen differ too much?

I will be involved in a plant disease produced by a bacterial pathogen, Acidovorax citrulli. I've previously worked with animal pathogens such as Haemophilus parasuis. Are there any big differences ...
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73 views

Why do many people prefer to grow microalgae in bottles?

I see many people growing Chlorella or Spirulina, types of microalgae, inside bottles... I see that they also insert CO2 into the bottles artificially... This question is basically comprised of two ...
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26 views

Why do we need to insert CO2 artifically to Microalgae pools?

Say I take a Chlorella culture and put it in mineral water inside a metal tub under the sun in the backyard --- Why do I need to insert CO2 to this? I mean, Chlorella that grows in freshwater lakes ...
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111 views

An experiment to test if a bacterial resistance gene is on the plasmid or chromosome?

So I have an E.coli strain phenotypicall resistant to the antibiotics ampicillin and rifampicin. How do I test if the AmpR gene is carried on a plasmid and not on the chromosome? In summary, I ...
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1answer
42 views

How many calories do gut bacteria burn?

It has become well-accepted that microbiota of the gut (a.k.a. gut bacteria) consume calories that are ingested and can have significant effects on energy metabolism in humans. For example, if you ...
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119 views

Whole gene sequence analysis to determine source infection [closed]

Is it possible to use whole gene sequence analysis to distinguish between a common source infection and a person-person disease transmission?
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73 views
+50

What makes worms' eggs float or sink?

In parasitology, while examining the stool sample, one important thing you do is test whether the eggs float on water or sink. We never did that since our coursework limits itself to microscopic ...
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74 views

Can bacteria metabolize fatty acids for fuel?

I'm not a microbiologist, but rather a physiologist curious about microbial metabolism. Much like humans bacteria can utilize glucose, but when it comes to long chain, medium chain, or short chain ...
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Are there bacteria that respire anaerobically in aerobic conditions?

There are facultative aerobic bacteria that switch to aerobic respiration in an aerobic state, but are there any organisms that would still perform anerobic respiration even when shifted to aerobic ...
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1answer
22 views

What are the differences among food poisoning, gastroenetritis and diarrhoea?

I have now studied a major chunk of my microbiology course without understanding the basic difference between the three. The terminologies seem to be confusing. Is it a necessity for food poisoning to ...
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1answer
30 views

Why are vegetations in Infective endocarditis common on the atrial side?

Robbin's Pathology says that vegetations of IE are more common on the atrial side in AV valves. In Liebmann Sack's Endocarditis, which is a sterile (non bacterial) type of endocarditis, the underside ...
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How can I make sure my UV-purificator kills bacteria?

I'm going to show a UV-purificator for water on science fair. How can I easily check if purificator kills most of the bacteria? I need a quick and possibly easy method.
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61 views

What would happen if there were no protists? [closed]

This is for an inquiry project for my biology 11 class. I need opinions from some professionals so please help out.:) if you dont mind also putting your name and what you do or what degree you that ...
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22 views

How does the snail shells' fertilizer compare to regular fertilizers?

May I ask about the quality of the fertilizer derived from the shells and their effectiveness compared to other fertilizers on the market ?
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1answer
38 views

How to transform snail shells into a fertilizer? [closed]

I heard that the chemical structure of the snail shell has elements that help in waste water treatment and in some cases as a fertilizer? What is the process used to render snail shells into ...
3
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1answer
37 views

Soft Agar Substitute

I've got petri dishes with a base agar layer ready for E. coli culturing. However, I do not have soft agar in which to dilute the E. coli before pouring it on to the plate. Any suggestions on what ...
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19 views

How to promote denitrifying microbe activity

I'm an amateur fresh-water aquarist looking at the problem of nitrate reduction (into largely-inert nitrogen gas) in a small-scale aquarium environment. The process of turning the byproducts of fish ...
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11 views

Function of NEZHA gene [closed]

What is the function of NEZHA? What effect does it have on microtubules and PLEKHA7? What happens after it has been knocked down?
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35 views

Earliest to use media after autoclaving?

I am autoclaving media to use later to inoculate yeast. How much time do I have to wait before using the media and adding the culture? Can I use it a few hours after autoclaving?
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How would one identify cellular transcription factors associated with a viral protein in a treated cell line?

I've been working as the computer guy for a microbiology lab for the past few months. I've always been interested in bench work, but my wet lab experience is rather limited and thus so is my ...
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28 views

Lifeforms concentrations of the categories of macromolecules, and Lipids

Lifeforms are formed of large, modular, organic molecules called macromolecules, large organic molecules called Lipids, and simpler molecules such as H2O. Macromolecules are commonly grouped into the ...
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23 views

Tardigrade genetic acceptance and experimentation?

Does this property of tardigrades, that when under extreme conditions they are more permeable and more easily accept sections of genes developed in other species, as I understand sometimes transfered ...
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Can tardigrades survive being eaten?

Compared to a tardigrade, the cockroach seems fragile. But can tardigrades survive the acidic environment of being eaten by most animals?