Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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Lifeforms concentrations of the categories of macromolecules, and Lipids

Lifeforms are formed of large, modular, organic molecules called macromolecules, large organic molecules called Lipids, and simpler molecules such as H2O. Macromolecules are commonly grouped into the ...
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Tardigrade genetic acceptance and experimentation?

Does this property of tardigrades, that when under extreme conditions they are more permeable and more easily accept sections of genes developed in other species, as I understand sometimes transfered ...
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Can tardigrades survive being eaten?

Compared to a tardigrade, the cockroach seems fragile. But can tardigrades survive the acidic environment of being eaten by most animals?
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Bacteria surviving a β-lactam antibiotic

What changes can occur in the cell wall of a bacteria for it to survive a β-lactam antibiotic? I think that because a bacteria possesses peptidoglycan in its cell wall, they are β-lactam sensitive, ...
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NMDS analysis - weird plots

I ran a NMDS analysis on 16S tag sequencing data on three different 16S regions, and would like to establish the regression coefficients (R, p) from this. All the data is stored within a combination ...
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using bioreactors for bacteria and yeasts

I have some questions about the OD to inoculate in a bioreactor and the OD for the induction using bacteria and yeasts (Pichia pastoris) to produce recombinant proteins. Which is the OD for the ...
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Why do bacteria die in a high sugar content environment?

I was reading some questions in the test bank. Then in Chapter 27, I wonder why the answer is A. (undergo death by plasmolysis). Is the high concentration of sugar that kills them or the effects of ...
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Is urine sterile? A reference request for evidence supporting sterility

There is a seemingly unfounded mantra that a person's urine is sterile who is not suffering from a UTI. So far I have found convincing evidence to the contrary; urine is not sterile. This poster ...
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How is a bacterial strain defined?

When a species of bacteria is referred to by its strain, are they a clone of single founder or is a certain amount variation allowed?
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30s ribosomal Inhibitor (Antibiotic)Question

I have a question regarding the mode of action of 30s ribosomal Inhibitors (antibiotcs) - tetracyclines. According to some online resources, the antibiotics enter the A-site, which prevents other tRNA ...
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How to identify genes in Ralstonia that synthesize PHB and promote granule formation?

The compound polyhydroxybutyrate (PHB) is of considerable industrial interest as a biodegradable substitute for plastic. PHB is synthesized from glycerol by the bacterium Ralstonia eutropha. PHB ...
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How long can you effectively store a glycerol stock at -20 degrees Celsius?

I know that glycerol stocks are typically kept in a -80 °C freezer, however there are some people who do not have access to such equipment. How long would you be able to keep a glycerol stock at ...
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How do microbes develop resistance to anitmicrobial peptides?

I would like to better understand how bacteria use the "strategy" of alternations to lipid A and membrane proteins in order to resist antimicrobial peptides of the immune system? It is my ...
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Of people who develop Alzheimer disease, are those people genetically predisposed to it?

I have read a lot lately about microbiological pathogens that are found in blood vessels in the brain of patient's with Alzheimer disease (positive association). So, I am confused whether there are ...
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32 views

How to obtain virus samples?

I'm trying to observe the behavior of simple viruses in different environments. I'm just looking for simple viruses like the common cold and the flu virus nothing major. Is there a way to obtain them? ...
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42 views

How exactly are petri dishes used (e.g. in medicine)? [closed]

I know that petri dishes with a growth medium are used to grow micro organisms. I guess this works as follows: The petri dish has to be kept sterile. To make it easier, I guess one could cool it. ...
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Can Biologists identify all viruses?

I went to the doctor today with my girlfriend, and the doctor said that she had a virus but doesn't know which one and she should let the infection heal with some rest. The fact that the doctor ...
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What is the advantage of using plant-derived antibacterials rather than bacteria-derived antibacterials?

So obviously we have a big problem with antibiotic resistance. Most of our antibiotics originate from bacteria themselves (or are synthetic variations on scaffolds which originate from bacteria). I ...
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How do cell repair mechanism ratios change as they age?

I have seen that embryonic stem cells are shown to use homologous repair for double strand breaks rather then non-homologous end joining (NHEJ). [1] I am wondering if something also happens to a ...
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Microbiology- Pseudomonas aeruginosa, BSAC data

I tested Pseudomonas aeruginosaagainst various antibiotics, using Stokes and Kirby-Bauer. When I compared my results with BSAC data it was completely different (understandable) but why for a lot of ...
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Values of Miu_max and Ks from parameter estimation?

Background I have this simple biomass growth model: $$ \mu = \mu_{max}\cdot \left(\frac{S}{K_S+S}\right) \cdot \left(\frac{1}{1+S/K_{iS}}\right) \cdot \left(\frac{K_{iP}}{K_{iP}+P}\right) \\ ...
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Relationship between turgor pressure and osmotic pressure?

I would like to know if there is a relationship between osmotic pressure (inside and outside of a cell) and turgor pressure. If so, is there a way to formalize it mathematically? Thank you in ...
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63 views

Experimental Analysis: What are possible reasons for this increase in N₂O production?

My professor wanted us to each conduct an experiment for class on something we thought would interesting. My experiment was very simple, but I'm not sure how to interpret my results. (Please note that ...
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How would substances that have anti bacterial characteristics interact with yeast? [closed]

This question has its origins at home brewers where the question was asked how would substances with anti bacterial qualities interact with yeast a fungus? No one at home brewers was really sure so I ...
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Oral Fordyce spots

This is an image of a Fordyce spot I found on Googling. I don't know how to interpret this image. Is this an image of a single Fordyce spot? Single as in a single spot when viewed with the naked ...
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difference between “Petri dishes” and “Petri plates”? [closed]

In microbiology, we often hear "we use Petri dishes to prepare our cultural media". Which is correct — "Petri dishes" or "Petri plates"?
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How sterile is working next to a bunsen burner?

When I was still doing lab work, many people would just wear gloves and work next to a bunsen burner because the clean benches were all in use. This was mostly for plating bacteria like Bacillus ...
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Can bacteria release free DNA into their environment?

Natural transformation AKA natural competence involves the uptake of DNA into a competent bacterium (for horizontal gene transfer or as a food source). My question is about where this extracellular ...
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114 views

Why does chlorination still work?

Chlorination has been used for over a century to disinfect water supplies. Why haven't microorganisms evolved immunity to this chlorine by now?
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Favored Conditions of Bacterial Growth

I have read that bacteria "thrive" in warm places. Naturally, I am very interested in why this is the case. Humans for instance thrive also in relatively warm conditions if it's too cold or too warm ...
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Why a particular species of bacteria give rise to particular type of colony? [closed]

Bacterial colony varies in form, elevation, margin, opacity, chromogenesis etc. What gives definite character to a colony and what is the source of all the diversity? Is the reason similar to that of ...
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26 views

Why would a bacterial population show initial growth when it is in unfavorable growth conditions?

This figure shows the anaerobe E. faecalis grown in aerobic conditions, E. coli grown in restricted conditions that are not specified. Why do they show an increase in abundance initially? The black ...
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What exactly are polyphenols and what benefits do they provide to humans?

I say somewhere that they are antioxidants, but I was wondering if the two terms are synonymous or if that is just one of many things polyphenols can do.
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Two different sized colonies from the same species of bacteria. What does it mean? [closed]

I have got two different sized colonies in a plate of Salmonella Paratyphi, identified by biochemical methods. What does two different sized colonies mean? What kind of question can I investigate in ...
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Why is 70% ethanol preferred for aseptic techniques?

Are other concentrations (say 80%) less effective,or is this just for convenient manufacturing? Is the concentration chosen only because it is less volatile than 100 percent ethanol and hence safer?
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How do you interpret this microbiology/ bacteriology research figure?

This is a figure from a research article that I am to do a senior presentation for. It is showing bacterial replication of an enteric bacteria population. The researchers hypothesize that the ...
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Difference between protozoa, protists, protoctista?

Are these different classes of organisms or simply different names for the same?
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65 views

Why don't bacteria eat food leftovers?

I have thrown a dirty spoon after eating some pasta into one of my desk drawers (doesn't do me much honor). It stayed there for around a year. My house is warm and I think there is enough humidity for ...
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Plasmid choosing

To design a experiment in feeding of C. elegans. It has to choose a plasmid vector to insert the gene of interest that can feed to C. elegans. Many paper are using pL4440 for the feeding vector, ...
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Which Lactobacillales (Lactic acid bacteria — LAB) strains are capable of degrading starch?

I've been researching bacteria strains but am having trouble finding amylolytic LAB strains with amylolytic and lactic acid producing character. The only species I've found, that are capable of ...
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Symbiosis of Pseudomonas Fluorescens

Have a modest background in molecular biology and presently considering a project that requires some knowledge of botany. Specifically, I seek details on the epiphytic relation between the microbe ...
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Bacteria Replication

I learned this at a lecture, but somehow I have trouble in understanding this. It is said that the bacteria such as E.coli need 20 minutes to divide, but its chromosome require 40 minutes to multiply. ...
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Does a microwave oven disinfect food?

Imagine I am preparing food, just about to put it into a microwave oven, and some of it falls on the floor. Assuming it got some bacteria or other organisms (viruses?) on it, will the microwave ...
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Common genes and enzymes involved in pathogen entry into host

Many microbes like Salmonella, E.coli, Legionella pneumophila etc. enter host cells via cystoskeleton remodeling of the host cell. Do all microbes follow the same path or there are any other ways for ...
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How can E. coli affect C. elegans expression?

Plasmids can be transferred to E. coli. These transformed E. coli can be fed to C. elegans to silence its gene expression by RNAi. How can E.coli release RNAi to C. elegans? Even if we assume E. ...
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Counting of surface bacteria in real time

I'm fairly new to microbiology (okay, I'm forced to look into it for a group project). What I'm looking for is a way to quantitatively get an estimate of the number of microbes (bacteria and/or ...
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Why do some bacteria have an asymmetric replication?

Our teacher said that Bacillus subtilis has an asymmetrical replication fork. I know that this happens only in some strain of the bacteria . She asked us to find an explication for this mechanism , ...
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Why are viruses considered microbes?

My question is simple. Why is a virus considered a microbe? Considering a microbe is considered to be a "living" unit of life, which viruses are not.
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Nucleoside analogs that cause mutation

I'm confused with this explanation in my book: 2-aminopurine is incorporated into DNA in place of adenine but can pair with cytosine, so an AT pair becomes a CG pair. This sentence seems odd to me. ...
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Is there a good easy protocol for extracting proteins(enzymes) from fungal mycelium?

I quantify the enzyme (polygalacturanase)activity by DNSA method.To determine endo-polygalacturonase activity I'm searching for a good easy enzyme extracting method.Any suggestions for a suitable ...