Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

learn more… | top users | synonyms

5
votes
1answer
47 views

Scale up of Bacterial growth from 250mL to 1-5L?

I am currently growing up a specific strain of E.Coli with a knockout in 40mL of growth medium (LB) in a 250mL shaker flask... My ultimate goal is to scale up this process to a 1-5L large scale setup ...
4
votes
2answers
309 views

What happens to lactic acid I eat?

Let's say I drink some Kefir Milk, what happens to the lactic acid in the Kefir Milk that has entered my digestive system?
0
votes
1answer
84 views

How HIV Affects and Its Treatment using Combination Therapy

Can someone please help me with the following questions. I've written my specific questions right after the text question. Human immunodeficiency virus (HIV) is a retrovirus. Its genome is a single ...
4
votes
2answers
198 views

How many different protein coding genes are in the Human Biome?

There are approximately 20k protein-coding genes found in the human genome. This number is presumably very small when considering all the genomes found in the diverse microbes associated with the ...
3
votes
1answer
31 views

How does sucrose protect bacterial cells in lysozyme solutions?

I have a microbiology question. When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe plasmolysis - the cytoplasmic membrane detaches from the cell wall due ...
4
votes
1answer
23 views

Re-solidify disturbed agar plates

I am stuck in trouble while pouring LB agar onto my petri dishes. I had to add kanamycin to my plates just before it started to solidify, out of forgetfulness, which I tried to mix using the tip. The ...
3
votes
2answers
1k views

Why do kefir grains stop growing in soy milk?

Kefir is a fermented milk drink made with kefir grains. It usually is prepared by inoculating cow, goat or sheep milk with kefir grains. I would like to prepare the drink with soy milk, which worked ...
5
votes
1answer
71 views

Sugar as a defence against bacteria?

An answer on another SE site mentions that sugar "at a certain level acts as a preservative". I've always been taught that microorganisms eat sugar and expel acids, that is why sugary food are ...
14
votes
3answers
734 views

Do probiotics survive digestion?

Pretty much this. I've been wondering if any of the yogourt and other "health" foods containing living probiotic cultures survive digestion to populate our intestines? If so, is there peer-reviewed ...
4
votes
1answer
57 views

Beetroot white skin mould

Does a mould make a beetroot poisonous or inedible ? Lemon's mould for instance, makes penicillin, but it's green there, and I'm allergic to penicillin, would the white mould produce penicillin too? ...
4
votes
2answers
378 views

Does making yogurt from non-pasteurized milk work against possible disease bacteria?

In the past, when there was no pasteurization, could making yogurt from milk lower the chance of getting infected by bovine tuberculosis (or other diseases from infected milk)? For example, would ...
0
votes
1answer
59 views

Prokaryotic Ribosomes

During bacterial protein synthesis, when ribosomes attach to the cytoplasmic membrane and form a polysome, to what molecule are the ribosomes attaching? Is the polysome held to the cytoplasmic ...
1
vote
0answers
25 views

What is Mycoplasma Lyo medium?

Used for instance in this article The role of genital mycoplasmas as pathogens - - are generally neglected by diagnostic laboratories in the United Kingdom, possibly due to the lack of a ...
3
votes
1answer
123 views

For people who work in microbiology labs, what software do you use to manage your strains?

It's just a small microbiology lab that currently records everything on paper, and there's quite few mutants as well. Is Excel commonly used for this sort of thing? Or is there a better software to ...
5
votes
1answer
58 views

Are Lambic beers the product of quorum sensing?

Lambic beers are a Belgian specialty which is still made by open-air "inoculation" by airborne microbiota first, followed by additional yeasts, LAB bacteria and more joining in at the time of ...
1
vote
1answer
56 views

How do penicillin resistant bacteria grow slower in the presence of penicillin?

We put 2 flasks inoculated with Bacillus cereus in 37⁰C: one with 100μg/ml penicillin + 50μg/ml chloramphenicol and the other without penicillin. We found that the OD is higher in the one without the ...
1
vote
1answer
50 views

Science experiments at home with Minimal Cost

What is a good recipe for making agar at home for a minimal cost? Are there materials that could be found in the kitchen cabinet?
0
votes
0answers
33 views

Locally sterile immune response in paradoxical IRS coupled with hypersensitivity I

The king of sterile immune response is antibodies; while in unsterile response - T lymphocyte. I think there can be two types of IRS: either abnormalities with T or B cells. Assume the here the case ...
2
votes
1answer
54 views

Anaerobic respiration choice in E. coli

Under anaerobic conditions E. coli has two options to generate ATP: fermentation (substrate-level phosphorylation), and respiration (proton gradient, chemisomotic phosphorylation). Which is favored? ...
3
votes
2answers
99 views

Highly spherical Radiolaria; does this image accurately depict the organism?

If I understand correctly, Cenosphaera spp. is a type of Radiolaria, which is a type of zooplankton. The following is an image of Cenosphaera spp. is from the supplementary material (Figure S2) of ...
0
votes
0answers
20 views

Can cyclical fever be connected with Balantidium coli?

This is a theoretical question about what can cause cyclical fever with B.coli infection. Lifecycle here from Dickson book: I am not sure if B. coli can cause cyclical fever itself. There is ...
6
votes
1answer
183 views

What is the effect of garlic on intestinal flora?

Now that we can compare whole microbiomes, has there been investigations on the effect of garlic (or diallyl disulfide, its main ingredient) on intestinal flora? From the proven antimicrobial ...
1
vote
1answer
37 views

Can Alzheimer's disease be caused by Slow Prion infection?

I found this claim in my study materials with explanation of amyloi plaques, in comparison to CJD (Creutzfeldt-Jakob Disease). Its characteristic are Long doubling time of at least 5.2 days (I ...
1
vote
1answer
19 views

Can Rubella virus pass into brain stem?

B19 virus (about 20 nm) can; which I think is partially because of its small size; not sure of genomics has something to do with the passing. Rubella's (also called German measles) size is then again ...
2
votes
1answer
30 views

How does the MMR vaccine affect lymph nodes in preventing measles?

I am trying to understand this statement about the Measles part of the MMR (Mumps, Measles and Rubella) vaccine Measles prevention: MMR (AB protect during primary and secondary viremia) Measles ...
0
votes
0answers
15 views

Direct spread of Parvovirus B19 from blood to Brain stem and CSF

Parvovirus can spread in blood (viremia) to bone marrow. It is detected in some stages of infection in Cerebrospinal fluid (CSF). However, I am thinking if it can spread directly with facilitated ...
3
votes
1answer
52 views

What is “bacto” peptone?

Standard recipes for yeast medium often include "bacto-peptone". Is this the same as bacteriological peptone? Is there an authoritative source that spells it out?
1
vote
0answers
25 views

Regulation of Cra protein level in E coli

Catabolite Activator/Repressor, Cra protein (formerly known as Fructure Repressor FruR) plays a significant role in central carbon metabolism of E coli. Its activity is inhibited by ...
2
votes
1answer
178 views

Where would Saccharomyces cerevisiae be found in highest concentrations in environment?

I have bakers asthma specifically to 'Sc' yeast, and know to avoid bakeries, breweries, etc... I have figured out to not disturb rotting leaf piles, and fermenting bark mulch. Where else? What ...
0
votes
1answer
38 views

Tc and Th1 interaction and viral immune response

Tc is T cell which can give T killer cells and T helper cells. T helper cells (Th1) see the pathogen presented by antigen presenting cells (dendritic cells and macrophages). They then secrete antigens ...
1
vote
0answers
39 views

Vitamin D oral intake, transportation and absorption

Several factors affecting vitamin D and its active form absorption and storage acidity of stomach (not significant effect) cytopathic effects of viruses cytopathic effects of bacteria where the ...
4
votes
1answer
545 views

Why do different bacteria have different shapes?

Why do different bacteria have different shapes? Is it only related to their function?
1
vote
1answer
45 views

Any forms of pneumonia that can be caught?

I am aware of the fact that there are several different types of pneumonia. However, I am wondering if any of these types of pneumonia are contagious from person to person. Is this possible?
6
votes
1answer
548 views

How to obtain bacteria samples at home?

As the original question went from hold to closed, I thought I would write up a more appropriate question. How should one go about getting bacterial samples to look at under a microscope at home? ...
1
vote
1answer
30 views

To have TBE vaccine during allergic times with mild asthmatic symptoms?

My assistant says that if you have asthmatic symptoms you cannot have TBE (Tick Born Encephalitis) vaccine, although you are living in the active zone of TBE. Cases mild allergic symptoms allergic ...
2
votes
0answers
153 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
1
vote
0answers
34 views

algae lamp questions?

I would be wondering if you help me if you know about algae lamps please give me detail about it and answer my questions This is my questions? how the algae will charge the battery in day?(process) ...
1
vote
1answer
44 views

Why PPD test in patients without M.tuberculosis often slightly positive?

I got this question which I interpret as Why PPD test in patients without M. tuberculosis often have slightly positive PPD reaction i.e., a lifelong slightly positive skin test reaction? because ...
1
vote
1answer
34 views

Can SIRS occur without Sepsis from Infection?

I am thinking this figure It suggests me that there has to be Sepsis that infection can lead to SIRS. I am thinking particularly the pathogenesis of Cryptococcus neoformans where respiratory ...
5
votes
1answer
52 views

Is a sequential double transformation acceptable?

Standard protocol states having two compatible vectors being transformed simultaneously during the same procedure. I've come across a situation in which transforming one vector, obtaining results, and ...
1
vote
1answer
55 views

How do Gram + bacteria use a proton gradient for F-type ATPase?

Does anyone know of any papers showing how Gram positive Fermiculates or Actinobacteria use a H+ gradient for an F-type ATPase, It seems impossible since there is no outer LPS membrane to maintain ...
1
vote
1answer
33 views

Cells created using differently aligned proteins

I remember reading that scientist were making cells (I assume bacteria), that used differently oriented proteins to create a whole new class of life. Because apparently right and left aligned proteins ...
0
votes
1answer
28 views

Can inhibition of lymphocytes migration be a direct cause of chronic inflammation?

Here is the original slide: I am thinking about the "cord factor" sentence in a more general case. Assume you inhibit Leucocytes migration. How does this lead to accumulation of macrophages in the ...
0
votes
0answers
19 views

What is the relation between HBeAG and Anti-HBc

I am analysing this picture about acute hepatitis, viral infection of HBV, where are standard antibodies and antigens of HBV: HBsAg serum antigen HBeAg some antigen in blood anti-HBc antibody in ...
1
vote
2answers
46 views

What's the fastest legal way for the nutrients in a dead body to re-enter the ecosystem?

Apologies for weirdness. Of the various legal methods of disposing of a human corpse, which one ensures the nutrients which compose the body get back into the wider ecosystem the fastest? Unless ...
1
vote
1answer
22 views

How does HBeAg change to Anti-HBe in acute hepatitis

I am thinking this figure which can also be drawn like this How does HBeAg change to Anti-HBe? There are some triggers that stimulate Anti-HBe production after HBeAg level is done. I think ...
3
votes
3answers
83 views

Why do 6-8% of diphtheria patients do not develop natural immunity after being affected?

I am thinking of why some patients do not have natural immunity after exposure to the A-B toxin of diphthria. I think the A-B exotoxin is the key factor causing this disease and should trigger memory ...
1
vote
1answer
48 views

Can a fungus become resistant to a chemical such as Potassium Permanganate?

A friend used potassium permanganate solution to treat tinea on the hands/feet but after some initial success, the tinea seems to be making a comeback. Could the fungus develop resistance to potassium ...
1
vote
1answer
53 views

Why bacteria produce light?

After seeing this video I am curious to know why the free living bacteria produce light. What advantage will they have ? Or is it just an "unintentional" result of one of their pathways ?
2
votes
1answer
82 views

What kind of a microscope do I need to see cell organelles?

I would like to study cells and looking for a microscope that would allow me to see: groups of cells individual cell cells organelles I would like to target insects and mammal tissue. I would be ...