Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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What is the common diet of food-related IBS patients? Besides most patients being lactose intolerant, what other intolerances do they face?

I am currently writing a paper on irritable bowel syndrome, and I was wondering what the common diet of a patient with food-related IBS consists of. I've already begun discussing the pathogenesis of ...
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92 views

Fermentation to acetic acid

How much time is needed for the fermentation to acetic acid production cycle, and the conversion of apple vinegar in a sealed container incubated at 37 °C? Should be in the fully closed? What is ...
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46 views

Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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359 views

Does “Garbage Enzyme cleaner” contain cleaning enzymes?

There are many recipes which call for using fruit peel fermentation process to make a concentrated enzyme cleaner solution, some variations call to add bakers yeast. So the question arises that does ...
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151 views

Why would yeast grow but not produce alcohol?

I'm studying for my intro to microbiology test and I have a question asking why a culture of yeast responsible for producing alcohol would be growing, but not producing alcohol? The multiple choice ...
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77 views

How exactly can dsRNA be introduced to a cell?

Is it just by viruses or are there other means by which it gets into cells, such as plasmid uptake?
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85 views

Are all functions of a human cell known? [closed]

Please bear with me as I'm intruding into your world from a computer science background. In programming, once you have created a program, you know all functions of that program. Thus, 100% knowledge ...
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40 views

Alkaline Phosphatase

What is the predominant purpose of Alkaline Phosphatase in skeletal muscle fibers and liver cells. I know that it is a hydrolase enzyme that speeds up the degredation of proteins, lipids, starch and ...
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75 views

Why is the ebola virus so intense now?

So i'm looking into the ebola crisis and it seems the death toll is really getting crazy. I understand that it's a cytomegalovirus and that it basically overwhelms the immune system due to it's size ...
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31 views

Does adding antibiotic after 5-10 mins of innoculation affect the protein yield or growth?

I've asked a lab colleague the same question. She said, it would loosen the bacterial cells in the LB medium and plasmids would come out. Is that true? and why?
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What do we know about the cellular structure, processes, environment, and immediate ancestors of the last universal common ancestor (LUCA)? [closed]

I am up for all scientifically sound speculations, and sources are highly welcome. I've looked into this quite a bit myself via scholar.google, the wiki article, and /r/askscience. I'm really looking ...
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449 views

What is the mechanism of oxygen uptake in E. coli?

How does E. coli uptake oxygen? Most of the literature I found is concerned with response to oxygen level supplied in the medium, as opposed to how much is actually transported inside. Can they shut ...
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2answers
283 views

What type of photosynthesis is performed by phytoplankton?

I am not a biologist, but I know there exist three variants of photosynthesis, namely C3, C4 and CAM. I would like to know what type is used by the ocean's phytoplankton? It might also be that ...
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2k views

Why do yeast ferment Equal sweetener

I had my students conduct a fermentation experiment manipulating sugar amounts (number of sugar packets in solution) and measuring CO₂ production via capture in a balloon and measuring circumference ...
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170 views

Answer the questions in one word with help of clues and images given [closed]

Name the things associated with Antonie Van Leeuwenhoek - (6 pictures are given for help and four of them are associated with these clues) a)Leeuwenhoek was the first to describe these and hence ...
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1answer
403 views

What do you call a virus when it's cured?

When a cure has been found for a virus, can it be called such anymore? Virus implies it's something you've contracted that you just have to live with until (hopefully) your body can overwhelm and ...
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999 views

What is this microscopic image from Peter Gabriel's New Blood album cover?

This image appears on the cover of Peter Gabriel's album New Blood. It appears to be a photograph of some microscopic biological cell. But it doesn't look like a blood cell to me. What kind of ...
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61 views

Rhizosphere vs. Endorhiza?

In microbiology and the naming of the various areas of the plant as it relates to microbial inhabitance, I am confused as to the difference between the terms endorhiza and rhizosphere. In this case I ...
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58 views

Did the Britons 100 years ago have different intestinal flora and fauna?

Note: this is not a question about history, but about human digestive system over time :) I know a lot of colleagues who traveled for business trips to India. All of them caught terrible diarrhea ...
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1k views

Why do different bacteria have different shapes?

Why do different bacteria have different shapes? Is it only related to their function?
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1answer
617 views

Can Naegleria fowleri enter through your ear?

Is it possible to get infected with the amoeba, Naegleria fowleri, through your ears, or is the nose the only way it can enter to cause infection? How commonly is it found in your stool as well?
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69 views

What does it mean to perform in vitro experiments with mutant bacteria?

I came across the following sentence while reading the paper: Nanchen, Annik, et al. "Cyclic AMP-dependent catabolite repression is the dominant control mechanism of metabolic fluxes under glucose ...
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Switch gut microbiota due to diet and effect on calorie uptake

I have seen peer-reviewed papers mentioning the daily changes in gut microbiota composition according to dietary changes. See for example this paper: http://genomebiology.com/2014/15/7/R89 My ...
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3answers
120 views

Why HIV patients have greater virulence of M.tuberculosis?

I am reading Murray Microbiology book. Some facts M. tuberculosis is an intracellular pathogen. At the time of exposure, M. tuberculosis enters the respiratory airways and infectious particles ...
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1answer
78 views

What is the expected effect of pH on the activity of a fungal pectinase?

I am working on an enzyme assay for a fungal pectinase.I assayed the enzyme in different buffers from pH 1-12.5 However,the enzyme has good activities starting from pH1-10.5. Is it possible to have ...
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1answer
96 views

Is there a difference between drinking water straight from the tap compared to leaving it to sit?

An idle question - if a glass if filled from the tap and consumed immediately, it contains some dissolved oxygen from the physical process of moving through the pipes & tap. If left out ...
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1answer
199 views

DpnI over-digestion

We have a long protocol that we are optimizing that includes DpnI digestion of a PCR product (to remove any of the template DNA if it's methylated, and while we're not certain in the blind tests, ...
3
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1answer
38 views

Can a plate with incompletely filled LB agar be used?

I am falling short of LB agar plates and I have a plate with less LB agar poured. I meant, the plate is not fully filled with the agar (it was the last plate I poured the medium into). Some part of ...
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71 views

Prokaryotic Ribosomes

During bacterial protein synthesis, when ribosomes attach to the cytoplasmic membrane and form a polysome, to what molecule are the ribosomes attaching? Is the polysome held to the cytoplasmic ...
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92 views

Organisms in food deposits

I have just discovered that my kitchen sink pipe has been leaking for quite some time (possibly months) which has resulted in water and food deposits collecting in my cupboard. I smeared some of the ...
3
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1answer
98 views

Consumption of NAD+ in glycolysis

Out of 10 steps in glycolysis, only one reaction- Glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (PGP), uses NAD+ and thereby producing NADH. Furthermore, this very step is solely ...
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3k views

Calculation of the bacterial growth rate from a spectrophotomer growth curve

Typically the microbial growth in liquid cultures is monitored by turbidity. Data is obtained with a spectrophotometer to measure optical density at 600nm. The slope of the bacterial kinetic curve in ...
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1answer
41 views

What is a good review book/source for microbiological diagnosis?

I am trying to find the diagnosis figure for different bacteria where phases are illustrated. I am using at the moment: 1) First Aid for the USMLE Step 1 2013 and 2) Medical Microbiology by Murray et ...
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60 views

Can we attack viruses by attaching proteins and such to their shells?

Haven't looked into this to very long, but an initial search didn't return anything. HIV uses certain co-receptors to enter our cells. Could we flood the blood stream with specially designed ...
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Can a fungus become resistant to a chemical such as Potassium Permanganate?

A friend used potassium permanganate solution to treat tinea on the hands/feet but after some initial success, the tinea seems to be making a comeback. Could the fungus develop resistance to potassium ...
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65 views

Use of salt as preservative

I know that salt is used as preservative as it dehydrates microbes. Is there any other advantage like - altering pH or inactivating microbial enzymes ?
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29 views

What is the specific use of a capsule in E.coli?

In evolutionary terms, will the capsule cease to exist someday or will it improve ? Does it provide any boost to the organism in any way which harms us ? Can't we remove capsules or engineer E.coli ...
5
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1answer
77 views

Scale up of Bacterial growth from 250mL to 1-5L?

I am currently growing up a specific strain of E.Coli with a knockout in 40mL of growth medium (LB) in a 250mL shaker flask... My ultimate goal is to scale up this process to a 1-5L large scale setup ...
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492 views

What happens to lactic acid I eat?

Let's say I drink some Kefir Milk, what happens to the lactic acid in the Kefir Milk that has entered my digestive system?
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1answer
95 views

How HIV Affects and Its Treatment using Combination Therapy

Can someone please help me with the following questions. I've written my specific questions right after the text question. Human immunodeficiency virus (HIV) is a retrovirus. Its genome is a single ...
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2answers
268 views

How many different protein coding genes are in the Human Biome?

There are approximately 20k protein-coding genes found in the human genome. This number is presumably very small when considering all the genomes found in the diverse microbes associated with the ...
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1answer
179 views

How does sucrose protect bacterial cells in lysozyme solutions?

I have a microbiology question. When we put bacterial cells in sucrose solution with concentration higher than 0.5M we observe plasmolysis - the cytoplasmic membrane detaches from the cell wall due ...
4
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1answer
42 views

Re-solidify disturbed agar plates

I am stuck in trouble while pouring LB agar onto my petri dishes. I had to add kanamycin to my plates just before it started to solidify, out of forgetfulness, which I tried to mix using the tip. The ...
3
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2answers
3k views

Why do kefir grains stop growing in soy milk?

Kefir is a fermented milk drink made with kefir grains. It usually is prepared by inoculating cow, goat or sheep milk with kefir grains. I would like to prepare the drink with soy milk, which worked ...
6
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1answer
106 views

Sugar as a defence against bacteria?

An answer on another SE site mentions that sugar "at a certain level acts as a preservative". I've always been taught that microorganisms eat sugar and expel acids, that is why sugary food are ...
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Do probiotics survive digestion?

Pretty much this. I've been wondering if any of the yogourt and other "health" foods containing living probiotic cultures survive digestion to populate our intestines? If so, is there peer-reviewed ...
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131 views

Beetroot white skin mould

Does a mould make a beetroot poisonous or inedible ? Lemon's mould for instance, makes penicillin, but it's green there, and I'm allergic to penicillin, would the white mould produce penicillin too? ...
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2answers
455 views

Does making yogurt from non-pasteurized milk work against possible disease bacteria?

In the past, when there was no pasteurization, could making yogurt from milk lower the chance of getting infected by bovine tuberculosis (or other diseases from infected milk)? For example, would ...
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What is Mycoplasma Lyo medium?

Used for instance in this article The role of genital mycoplasmas as pathogens - - are generally neglected by diagnostic laboratories in the United Kingdom, possibly due to the lack of a ...
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256 views

For people who work in microbiology labs, what software do you use to manage your strains?

It's just a small microbiology lab that currently records everything on paper, and there's quite few mutants as well. Is Excel commonly used for this sort of thing? Or is there a better software to ...