Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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Looking for raw cell videos from a light microscope

First off, I'm not looking for online lectures. I am trying to find creative commons licensed videos of live cells moving around without annotation or anything extra. I would prefer a few minutes of ...
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120 views

List of all reported bacteria

I would like to know were to download a table file with all reported bacteria and its corresponding PHYLUM, CLASS, ORDER and FAMILY. Basically, its the same table than this one from GOLD genomes but ...
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60 views

When might an inhibitor of bacterial transformation be useful?

I am part of a project elucidating some structures that are required for bacterial transformation. We have the opportunity to screen inhibitors of the system to stop it from functioning. I am not a ...
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57 views

How HIV Affects and Its Treatment using Combination Therapy

Can someone please help me with the following questions. I've written my specific questions right after the text question. Human immunodeficiency virus (HIV) is a retrovirus. Its genome is a single ...
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3answers
506 views

Why do yeast ferment Equal sweetener

I had my students conduct a fermentation experiment manipulating sugar amounts (number of sugar packets in solution) and measuring CO2 production via capture in a balloon and measuring circumference ...
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20 views

How to get conservation of vaccine candidates between hundreds of bacterial strains?

We have identified vaccine candidates via an ex-vivo RNA-seq approach. Next step would be to perform conservation of these candidates (about 20) between multiple bacterial strains (about 200). I would ...
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66 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
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474 views

Bacterial 16S rRNA PCR amplification with universal primers

I am doing an experiment to amplify the 16S rRNA gene from bacteria present in gut contents of fish. After extracting DNA, I perform one-step PCR with universal bacterial primers (27F, 1492R) and I ...
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36 views

What are the ingredients of Pheromone Trap using for controlling Fruit flies of cucumber?

Pheromone Traps are used for fruit fly control. But I have no idea which ingredients or chemicals are used for preparing Pheromone trap
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22 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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154 views

Alternatives to CFU plating for measuring number of viable cells?

I am hoping to measure growth rates of a bacterial culture in several growth conditions. I am concerned that these growth conditions may cause cell death, which would lead to a decreased ...
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35 views

Use of salt as preservative

I know that salt is used as preservative as it dehydrates microbes. Is there any other advantage like - altering pH or inactivating microbial enzymes ?
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80 views

explanation of conjugation experiment in bacteria

In this paper (On the expression of a structural gene) I am confused about what is being plotted in Figure 6. The x-axis contains the fraction of radioactive day and y-axis contains the enzyme ...
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116 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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35 views

Shortest route for a Nitrogen atom from fossil fuel to a protein in your body?

Millions of years ago fossil fuels originated from plants. A nitrogen atom from fossil fuel is now inside in a protein molecule in your body. What is the shortest path that the nitrogen atom ...
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69 views

At what temperature does DNA re-anneal?

If primers weren't present in PCR at what temperature would DNA re-anneal? I am wondering how primers manage to bind on DNA strands before DNA manages to anneal back together.
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34 views

algae lamp questions?

I would be wondering if you help me if you know about algae lamps please give me detail about it and answer my questions This is my questions? how the algae will charge the battery in day?(process) ...
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54 views

Need for proper air filter for bioreactor?

I am going to build a simple bioreactor for microalgae growth. I already have fluid filters pore size: 0.2um, diameter: 25mm https://nl.vwr.com/app/catalog/Product?article_number=514-4039 I wonder ...
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398 views

Why do different bacteria have different shapes?

Why do different bacteria have different shapes? Is it only related to their function?
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2answers
136 views

What are artifacts in microbiology?

What kind of objects can be qualified as artifacts? How do we distinguish such objects?
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68 views

Aerobe or facultative anaerobe organism that metabolizes acetate?

I'm assuming bacteria, but will take any suggestions. Organism must survive primarily on the acetate (plus trace elements), but I can give/take electrons, if necessary. Not interested in strict ...
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159 views

How do I measure bacterial growth in agar dishes (either by cell mass or by cell count)?

I am doing an experiment in which I am growing S. mutans in agar dishes, and I am not sure how I would measure the growth of the S. mutans. I am also not sure if I would do this by measuring cell mass ...
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50 views

What are some readily available, easy to test, bacteria?

I want to do a science fair experiment about how bacteria interact with 5-carbon vs. 6-carbon sugars, and I want a bacteria that is easy to test and is readily available. Any ideas?
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1answer
53 views

observed luminescence in a piece of plaice

A plaice fish bought for human feed has been cleaned and cooked. Some parts of the raw body have been left out of the fridge at about 22°C for about 15 hours. During the following night a ...
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31 views

What are the characteristic structures of bacillus M. tuberculosis and what they cause?

I answered to this question: In most forms of the disease, the bacillus M. tuberculosis spreads slowly and widely in the lungs, causing the formation of hard nodules (tubercles) in the ...
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48 views

Reference for writing an International journal in micro-biology/micro-organism field [closed]

Could someone do me a favor for any guidance (link) for writing an International journal in micro-biology/micro-organism field? Could you please lead me to another source instead of this site Thanks ...
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37 views

Why do fresh cavities form from the margins of sliver amalgam fillings on teeth?

Silver amalgam fillings predominantly contain silver a known bactericidal agent and mercury which a known toxin and has bactericidal property. So how is it that the plaque bacteria survive near the ...
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45 views

What is it the most common reason for community acquired pneumonia?

This is a difficult question because there are many types of community-acquired pneumonia (CAP): typical acute, atypical acute and chronic. Most common microorganisms are also different among ...
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67 views

Why does the mixture of infant formula with saliva provide an ideal breeding ground for disease-causing micro-organisms?

From: http://www.nal.usda.gov/wicworks/Topics/FG/Chapter4_InfantFormulaFeeding.pdf Discard any infant formula remaining after a feeding. The mixture of infant formula with saliva provides an ideal ...
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29 views

How to Diagnose this hepatitis A, B and C case?

Male patient who is working in the hospital and may be infected with contaminated blood had hepatitis with genome IA 5 years ago. His hepatitis A at that time was confirmed with the rise of anti.HAV ...
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45 views

Is this necrotizing skin infection caused by Staphylococcus aureus?

A 5-year-old boy returns from summer camp with several minor cuts and abrasions on legs. He has fever and extensive cellulitis, subcutaneous tissue and muscles are involved. Necrotic tissues were ...
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26 views

Elevated transaminases after blood transfusion?

This sparks HCV immediately in my mind. However, there may be other possibilities too. What can you deduce from elevated transaminases if you only know that the healthy adult patient received blood ...
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19 views

Can antiviral antibodies help viral infections which has many serotypes?

For instance Dengue virus has four serotypes. Infection by a different serotype causes only a more serious infection. 2) Which are the most common virus infections that has many serotypes?
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38 views

How can a respiratory infection lead to a skin infection?

Example of a case: 5 children develop a bright red rash on the face and turns violet after a few days and then disappears. Then maculopapular rash appears on the trunk, buttocks and extremities. It ...
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36 views

Can acid-fast M. tubercolosis obstruct alveoli?

I am not sure which word is the best here for colonisation of alveoli by acid fast-mycobacteria. I found this question, and I am getting confused by the term obstruction of alveoli because I tend to ...
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28 views

Which strains of C. diphteria are causing Diphteria toxin?

I got this question: Diphteria toxin is produced only by those strains of C. diphtheria that have which of the following characteristics? Encapsulated glucose fermenters lysogenic for b-prophage ...
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189 views

Factor causing Methicilin-resistance in MRSA?

I think the main reason is the natural selection that is causing methicillin-resistance. However, I am not completely sure what this means practically. Here, the original question: MRSA was isolated ...
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249 views

Can Naegleria fowleri enter through your ear?

Is it possible to get infected with the amoeba, Naegleria fowleri, through your ears, or is the nose the only way it can enter to cause infection? How commonly is it found in your stool as well?
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145 views

How to deduce if RNA virus infection or DNA virus infection

Is there any general rule to say this must be RNA virus infection and the other one DNA virus infection? Example of a case: 5 children develop a bright red rash on the face and turns violet after a ...
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86 views

Is this case Histoplasma capsulatum?

A 62­ year-­old male was admitted to the hospital complaining of shortness of breath. His chest X­-ray showed a rightsided cavitary lesions. His medical history indicated he had smoked one pack of ...
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99 views

Any food crops that could grow in a desert provided that they get fertilizer and water

I am working on an idea to provide nearly unlimited amounts of water in desert regions. To make this commercially viable. I would use the water to irrigate desert sand and grow crops. Given my ...
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60 views

Is iodised salt harmful to the lactofermentation process?

I was discussing sauerkraut recipes with someone on IRC a minute ago, and they warned me not to use iodised salt in the process. My first (and so far, the only successful) batch is one I made with ...
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376 views

How to obtain bacteria samples at home?

As the original question went from hold to closed, I thought I would write up a more appropriate question. How should one go about getting bacterial samples to look at under a microscope at home? ...
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767 views

Why do kefir grains stop growing in soy milk?

Kefir is a fermented milk drink made with kefir grains. It usually is prepared by inoculating cow, goat or sheep milk with kefir grains. I would like to prepare the drink with soy milk, which worked ...
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1k views

Why do cell membranes have a lipid bilayer?

Many cells have a cell membrane composed of two layers of lipids, why is it two layers and not just one? What purpose do the membranes serve?
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33 views

Do toxin producing strains of E. coli constantly produce toxin or only under certain conditions?

Background E. coli is prevalent everywhere but only some strains produce toxins harmful to humans, such as E. coli O157:H7. Questions 1) Would anyone know if these strains are always producing ...
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2answers
118 views

How to learn biomathematics?

I'm currently an undergraduate math student and researching on the internet I discover that exists an area called "biomathematics". Looks so interesting. They use the graph theory and topology to ...
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149 views

How to obtain bacteria samples?

Would anyone know how to go about obtaining samples of Salmonella or E. coli for purely educational purposes? Note: I just want to look at the stuff under a microscope.
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91 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
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2answers
86 views

E. coli, risk of infection?

There has been a warning about E. coli contaminated water in South Florida. Now I'm wondering are there empirical data or historic cases which show a correlation between E.coli levels in tap water and ...