Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

learn more… | top users | synonyms

3
votes
0answers
62 views

What is the advantage of using plant-derived antibacterials rather than bacteria-derived antibacterials?

So obviously we have a big problem with antibiotic resistance. Most of our antibiotics originate from bacteria themselves (or are synthetic variations on scaffolds which originate from bacteria). I ...
8
votes
1answer
939 views

What is the effect of garlic on intestinal flora?

Now that we can compare whole microbiomes, has there been investigations on the effect of garlic (or diallyl disulfide, its main ingredient) on intestinal flora? From the proven antimicrobial activity,...
21
votes
2answers
373 views

How does the microbial environment in your gut initiate?

Clearly, a zygote does not harbor any microbes. As it develops, and the alimentary canal tissue is differentiated, I logically assume that there is still no microbial activity in the fetus's gut. I'm ...
2
votes
0answers
43 views

How is the appropriately-stratified gut microbiome acquired in organisms performing horizontal transmission?

I am studying horizontal transmission of primary symbionts in insect reproduction. This reminded me of an earlier question I had asked, in which I learned that humans analogously acquired their ...
15
votes
2answers
236 views

Relationship between our microbiome and personalized nutrition

Recently, it has been asked whether there are 'metabolic types' between humans that can benefit from a sort of personalized nutrition. One answer suggested that one discerning factor could be the ...
2
votes
1answer
46 views

How many diseases can be linked to disruption in the microbiome of a human?

I was listing to the radio and heard recent research found a link between children and higher cases of asthma when certain bacteria are missing from the microbiome. How many other diseases can be ...
1
vote
0answers
35 views

How do cell repair mechanism ratios change as they age?

I have seen that embryonic stem cells are shown to use homologous repair for double strand breaks rather then non-homologous end joining (NHEJ). [1] I am wondering if something also happens to a cell'...
1
vote
0answers
16 views

Microbiology- Pseudomonas aeruginosa, BSAC data

I tested Pseudomonas aeruginosaagainst various antibiotics, using Stokes and Kirby-Bauer. When I compared my results with BSAC data it was completely different (understandable) but why for a lot of ...
1
vote
0answers
31 views

Values of Miu_max and Ks from parameter estimation?

Background I have this simple biomass growth model: $$ \mu = \mu_{max}\cdot \left(\frac{S}{K_S+S}\right) \cdot \left(\frac{1}{1+S/K_{iS}}\right) \cdot \left(\frac{K_{iP}}{K_{iP}+P}\right) \\ \frac{...
3
votes
1answer
548 views

Relationship between turgor pressure and osmotic pressure?

I would like to know if there is a relationship between osmotic pressure (inside and outside of a cell) and turgor pressure. If so, is there a way to formalize it mathematically? Thank you in advance,...
7
votes
1answer
118 views

How often does bacterial transformation happen?

I have been reading: M. Dröge, A. Pühler, W. Selbitschka, "Horizontal gene transfer among bacteria in terrestrial and aquatic habitats as assessed by microcosm and field studies", Biol. Fertil. ...
1
vote
2answers
24 views

How would substances that have anti bacterial characteristics interact with yeast? [closed]

This question has its origins at home brewers where the question was asked how would substances with anti bacterial qualities interact with yeast a fungus? No one at home brewers was really sure so I ...
4
votes
4answers
3k views

What is the difference between a spore and a cyst?

Last year I read a course in biology. During that course I learnt that Protozoa like Lamblia intestinalis or Entamoeba hystolitica form cysts in unfavourable conditions. This year I read a course in ...
10
votes
2answers
347 views

Can bacteria release free DNA into their environment?

Natural transformation AKA natural competence involves the uptake of DNA into a competent bacterium (for horizontal gene transfer or as a food source). My question is about where this extracellular ...
0
votes
2answers
342 views

Difference between protozoa, protists, protoctista?

Are these different classes of organisms or simply different names for the same?
0
votes
2answers
279 views

difference between “Petri dishes” and “Petri plates”? [closed]

In microbiology, we often hear "we use Petri dishes to prepare our cultural media". Which is correct — "Petri dishes" or "Petri plates"?
4
votes
1answer
337 views

How sterile is working next to a bunsen burner?

When I was still doing lab work, many people would just wear gloves and work next to a bunsen burner because the clean benches were all in use. This was mostly for plating bacteria like Bacillus ...
3
votes
2answers
251 views

How does Cro protein expressed by lambda phage kill its host?

I read that the DNA segment of lambda phage integrated in host DNA could switch between lysogenic state where cI represses the expression of Cro and lytic state where Cro expression takes over and ...
8
votes
1answer
129 views

Why does chlorination still work?

Chlorination has been used for over a century to disinfect water supplies. Why haven't microorganisms evolved immunity to this chlorine by now?
8
votes
3answers
1k views

Why is triclosan not considered an antibiotic?

Triclosan is a chemical often referred to as a "biocide" instead of an "antibiotic". However, its mode of action seems to suggest that it is an antibiotic. Triclosan binds to bacterial enoyl-acyl ...
1
vote
2answers
72 views

Favored Conditions of Bacterial Growth

I have read that bacteria "thrive" in warm places. Naturally, I am very interested in why this is the case. Humans for instance thrive also in relatively warm conditions if it's too cold or too warm ...
4
votes
0answers
77 views

Why a particular species of bacteria give rise to particular type of colony? [closed]

Bacterial colony varies in form, elevation, margin, opacity, chromogenesis etc. What gives definite character to a colony and what is the source of all the diversity? Is the reason similar to that of ...
2
votes
1answer
101 views

What are the differences and similarities between fimbriae and adhesins in microbes adherence factor?

Adherence factors in microbes is to attach to a host cell or to an extracellular matrix. So what is the difference and similarities between fimbriae and adhesins?
3
votes
1answer
67 views

Why do some bacteria have an asymmetric replication?

Our teacher said that Bacillus subtilis has an asymmetrical replication fork. I know that this happens only in some strain of the bacteria . She asked us to find an explication for this mechanism , ...
1
vote
1answer
30 views

Why would a bacterial population show initial growth when it is in unfavorable growth conditions?

This figure shows the anaerobe E. faecalis grown in aerobic conditions, E. coli grown in restricted conditions that are not specified. Why do they show an increase in abundance initially? The black ...
0
votes
0answers
18 views

What exactly are polyphenols and what benefits do they provide to humans?

I say somewhere that they are antioxidants, but I was wondering if the two terms are synonymous or if that is just one of many things polyphenols can do.
17
votes
2answers
4k views

Why is 70% ethanol preferred for aseptic techniques?

Are other concentrations (say 80%) less effective,or is this just for convenient manufacturing? Is the concentration chosen only because it is less volatile than 100 percent ethanol and hence safer?
2
votes
2answers
378 views

What does “cellular” mean in this context?

I came across a confusing word when I was reading a Scientific American story, “Controversial Spewed Iron Experiment Succeeds as Carbon Sink” (by David Biello). It goes like this: “One key to the ...
1
vote
0answers
49 views

Which Lactobacillales (Lactic acid bacteria — LAB) strains are capable of degrading starch?

I've been researching bacteria strains but am having trouble finding amylolytic LAB strains with amylolytic and lactic acid producing character. The only species I've found, that are capable of ...
2
votes
2answers
223 views

Why does sulfonamid not inhibit the growth of Rickettsia?

Sulfonamid is an antimicrobial agent, why has it no effect on the growth of Rickettsia?
1
vote
1answer
84 views

Why don't bacteria eat food leftovers?

I have thrown a dirty spoon after eating some pasta into one of my desk drawers (doesn't do me much honor). It stayed there for around a year. My house is warm and I think there is enough humidity for ...
3
votes
1answer
79 views

Plasmid choosing

To design a experiment in feeding of C. elegans. It has to choose a plasmid vector to insert the gene of interest that can feed to C. elegans. Many paper are using pL4440 for the feeding vector, ...
2
votes
3answers
350 views

Does a microwave oven disinfect food?

Imagine I am preparing food, just about to put it into a microwave oven, and some of it falls on the floor. Assuming it got some bacteria or other organisms (viruses?) on it, will the microwave ...
1
vote
0answers
30 views

Symbiosis of Pseudomonas Fluorescens

Have a modest background in molecular biology and presently considering a project that requires some knowledge of botany. Specifically, I seek details on the epiphytic relation between the microbe ...
2
votes
2answers
66 views

Bacteria Replication

I learned this at a lecture, but somehow I have trouble in understanding this. It is said that the bacteria such as E.coli need 20 minutes to divide, but its chromosome require 40 minutes to multiply. ...
8
votes
1answer
2k views

How can Valonia ventricosa cells get so big?

Valonia ventricosa are single celled algae that range between one and few centimeters. In rare cases they can reach sizes exceeding 5cm. They range from grass green, to dark green, and some are even a ...
2
votes
1answer
70 views

How can E. coli affect C. elegans expression?

Plasmids can be transferred to E. coli. These transformed E. coli can be fed to C. elegans to silence its gene expression by RNAi. How can E.coli release RNAi to C. elegans? Even if we assume E. ...
0
votes
0answers
15 views

Counting of surface bacteria in real time

I'm fairly new to microbiology (okay, I'm forced to look into it for a group project). What I'm looking for is a way to quantitatively get an estimate of the number of microbes (bacteria and/or ...
3
votes
2answers
2k views

Why are viruses considered microbes?

My question is simple. Why is a virus considered a microbe? Considering a microbe is considered to be a "living" unit of life, which viruses are not.
0
votes
1answer
41 views

Nucleoside analogs that cause mutation

I'm confused with this explanation in my book: 2-aminopurine is incorporated into DNA in place of adenine but can pair with cytosine, so an AT pair becomes a CG pair. This sentence seems odd to me. ...
1
vote
0answers
33 views

Is there a good easy protocol for extracting proteins(enzymes) from fungal mycelium?

I quantify the enzyme (polygalacturanase)activity by DNSA method.To determine endo-polygalacturonase activity I'm searching for a good easy enzyme extracting method.Any suggestions for a suitable ...
4
votes
1answer
660 views

How was the diversity between ethanol fermentation and lactic acid fermentation evolved?

Quite simply, some organisms metabolize glucose under anaerobic conditions via Glucose->(2) Pyruvic Acid->(2)Ethyl Alcohol. Some organisms, however, metabolize to lactic acid. When did such a process ...
2
votes
0answers
32 views

“Antibiotic resistance” equivalent in archaea for selection during cloning

I'm beginning to work with halophilic archaea and I'm trying to figure out a good way to select for cells that have taken up a plasmid. Obviously, one can't use antibiotic resistance since they are ...
1
vote
0answers
30 views

Enrichment of slow growing microbes: Large Test Tubes as an alternative?

Well, i would like to enrich bacterial and archaeal ammonia oxidisers from soil samples using Minimal Salt media. They are slow growing microbes. I would like to enrich them with the aim to produce ...
3
votes
1answer
592 views

Can the RNA in the HIV virus make viral enzymes without entering the nucleus?

If the provirus was not formed yet, can the virus make viral enzymes? (I know that it already has some, but supposing it doesn't)
1
vote
3answers
428 views

Can ampicillin resistant bacteria survive penicillin plate?

in my molecular bio class we were asked a trick question: If the bacteria has a plasmid with that grants it ampicillin ressistance, can the bacteria survive if placed in penicillin? I gave it a ...
2
votes
1answer
24 views

How can I interface cAMP signal or membrane potential from slime mold to an electrode?

I am very much interested in measuring cAMP (cyclic adenosine mono phosphate) signal and membrane potential from amoeba. Since I want to try this as a hobby, what are the basic steps I must do to view ...
8
votes
5answers
3k views

What's the aim of genetically modifying of foods/organisms?

On news, articles etc. experts talking about Genetically Modified Foods and Organisms often mentions about their disadvantages like, their potential to harm human health allergies may become more ...
0
votes
0answers
32 views

Soy kefir that never revitalises in animal milk

If I have kefir grains from goat and I make soy kefir and never revitalise the kefir grains in an animal milk, will that bacteria still be beneficial for the gut? What type of bacteria does it make? ...
9
votes
1answer
112 views

Reasons for the HIV-1 epidemic

So, the HIV-1 jump to humans occurred as early as the 1920s, but the AIDS epidemic didn't start until the early 1980s. Some things I don't understand about this: Why the delay? What is needed for a ...