Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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Survival of E. coli on agar plate at -20 degree C without glycerol stock

If I kept E. coli transformed with plasmid at -20˚C without making glycerol stock, will it survive? Actually I have kept it this way for 18 hours. What will happen with it? Will it grow in fresh ...
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Salvaging a plasmid from a cell culture stored at the incorrect temperature

I have E. coli with transformed plasmid on agar in a vial. It has been stored at -20 degree C without glycerol stock for 18 hours. This is a continuation of: Survival of E. coli on agar plate at -20 ...
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109 views

Mutation-immunity in Luria-Delbruck experiment

If experiments like those of Luria and Delbruck on E. coli and T1 phage are the main source of our confidence in the mutation-immunity model, is it then highly unlikely that there are other types of ...
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Non-monotonic knock-out effects in prokaryotes

Typically, when performing gene-knockout, the experimenters select one gene to remove/replace-with-junk and then see if the prokaryote can still undergo fission. If it continues to reproduce then the ...
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Vigorous shaking for HFR interrupted mating

I am trying to reproduce E. coli interrupted mating using an HFR strain, and I read that the cultures should be vigorously shaken at times to interrupt the DNA transfer from the F+(HFR) donors to the ...
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Does making yogurt from non-pasteurized milk work against possible disease bacteria?

In the past, when there was no pasteurization, could making yogurt from milk lower the chance of getting infected by bovine tuberculosis (or other diseases from infected milk)? For example, would ...
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How does the microbial environment in your gut initiate?

Clearly, a zygote does not harbor any microbes. As it develops, and the alimentary canal tissue is differentiated, I logically assume that there is still no microbial activity in the fetus's gut. I'm ...
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266 views

Aren't antibiotic resistant probiotics dangerous?

Multidrug resistant probiotics are often recommended by doctors in various cases. But since bacteriae can easily exchange genes by conjugation or other means they could promote the drug resistance of ...
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How do baby animals that primarily subsist on cellulose get their initial gut flora?

In the case of mammals like giraffes and koalas, is that bacteria common on the plants they eat so when a baby starts to try to stick something besides its mother's milk in its mouth, it can't digest ...
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Where can I find approximate rates of sequestration of CO2 for different species of algae?

For a study, I want to compare the rates of CO2 sequestration and fixation of a few different species of algae. I could not find any data on the sequestration rates. Any pointers to where I can find ...
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Is it plausible that eukaryotic organelles like flagella and cilia are the result of endosymbiosis with spirochetes?

This was a claim by Lynn Margulis explained over at this link. The sense organs of vertebrates have modified cilia: The rods and cone cells of the eye have cilia, and the balance organ in the ...
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Relationship between our microbiome and personalized nutrition

Recently, it has been asked whether there are 'metabolic types' between humans that can benefit from a sort of personalized nutrition. One answer suggested that one discerning factor could be the ...
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What's the aim of genetically modifying of foods/organisms?

On news, articles etc. experts talking about Genetically Modified Foods and Organisms often mentions about their disadvantages like, their potential to harm human health allergies may become more ...
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Do probiotics survive digestion?

Pretty much this. I've been wondering if any of the yogourt and other "health" foods containing living probiotic cultures survive digestion to populate our intestines? If so, is there peer-reviewed ...
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127 views

Are these Gram stain substitutions acceptable?

In the context of a Gram stain on a blood smear: Are the following acceptable substitutions and/or what differences could arise by substituting them? Using methylene blue instead of crystal violet ...
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What is the advantage of using starter cultures for growing bacteria?

Many DNA isolation and protein expression protocols contain instructions to use a starter culture of E. coli that is then used to inoculate the main culture. What are the advantages of using starter ...
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241 views

Can Naegleria fowleri enter through wounds into the bloodstream?

All the sources I've read said that Naegleria fowleri enters the brain through the nose. But lets say that someone had cuts on their arm or leg and they are swimming in water that is conducive to ...
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Antibacterial hand soaps (and related products); what are they good for?

From Wikipedia: Household use of antibacterials in soaps and other products, although not clearly contributing to resistance, is also discouraged (as not being effective at infection control). ...
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947 views

What is the distinction between F' plasmid and R plasmid?

Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...
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137 views

Is there an equivalent to “Fields Virology” for Bacteria?

I've gotten a staggering amount of use out of my copy of Fields Virology as a general reference for "getting me up to speed" on whatever pathogen I'm currently looking at. I don't know of a similar ...
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Are there animal models for Clostridium difficile that better replicate human infection than hamsters?

So I'm looking for some information on the infectious dose necessary to colonize a human with Clostridium difficile. There's no human challenge studies, and since it's not a foodborne pathogen, little ...
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Why is the microbial ecosystem of the gut so susceptible to disruption by pathogens?

From all accounts, it seems as if the Escherichia, Enterobacter, etc. that live and thrive in the human gut are pretty well entrenched. I know that these microbial populations are often analyzed as ...
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Is there any convincing evidence for the existence of nanobacteria?

The existence of nanometer scale microorganisms has been proposed and used explain several phenomena including morphological structures in a martian meteorite (ALH 84001) and implication in the ...
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How to understand influenza strain designations?

What do the strain designations for flu mean? For example avian flu is classified as H5N1, what do the letters H, ...
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Why is there now only one Salmonella species?

Once upon a time, I chanced upon an old microbiology book that detailed the rather colorful world of enterobacteria. Salmonella in particular stood out, as it seemed there were a lot of species: typhi ...