Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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Why bacteria produce light?

After seeing this video I am curious to know why the free living bacteria produce light. What advantage will they have ? Or is it just an "unintentional" result of one of their pathways ?
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39 views

Can germs develop surfactant resistance in a harmful way?

Germs can develop antimicrobial resistance. It is a problem serious enough to deserve an FAQ page on the WHO website (http://www.who.int/mediacentre/factsheets/fs194/en/). But how about surfactant ...
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88 views

How HIV Affects and Its Treatment using Combination Therapy

Can someone please help me with the following questions. I've written my specific questions right after the text question. Human immunodeficiency virus (HIV) is a retrovirus. Its genome is a single ...
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3answers
104 views

Assay for Beta-galactosidase activity in single cell microscopy

I'd like to be able to measure the activity of $\beta$-galactosidase in living cells with simple optical (maybe fluorescence) microscopy. Ideally I'd like to do a minimum of genetic engineering, and ...
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1answer
110 views

At what temperature does DNA re-anneal?

If primers weren't present in PCR at what temperature would DNA re-anneal? I am wondering how primers manage to bind on DNA strands before DNA manages to anneal back together.
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182 views

How to deduce if RNA virus infection or DNA virus infection

Is there any general rule to say this must be RNA virus infection and the other one DNA virus infection? Example of a case: 5 children develop a bright red rash on the face and turns violet after a ...
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1answer
80 views

Is iodised salt harmful to the lactofermentation process?

I was discussing sauerkraut recipes with someone on IRC a minute ago, and they warned me not to use iodised salt in the process. My first (and so far, the only successful) batch is one I made with ...
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2k views

Why do cell membranes have a lipid bilayer?

Many cells have a cell membrane composed of two layers of lipids, why is it two layers and not just one? What purpose do the membranes serve?
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“Oblong to Allantoids” … Is it valid?

I read on the text book: The characteristic of Dinemasporium are: superficial, cupulate to Discoid conidiomata with brown satae, Phialid conidiogenous cells that give rise to hyaline, oblong to ...
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200 views

What does “cellular” mean in this context?

I came across a confusing word when I was reading a Scientific American story, “Controversial Spewed Iron Experiment Succeeds as Carbon Sink” (by David Biello). It goes like this: “One key to the ...
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925 views

Is there a free alternative to Gelcompar for comparing banding patterns across multiple gels?

In order to run my microbial community samples from my experiments through DGGE, I was required to use multiple gels. Thus it is necessary to compare banding patterns across more than one gel. ...
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23 views

Molecular Cloning- Blunt end restriction endonucleases

I work in a microbiology lab where we do a lot of cloning. I have always used restriction endonucleases to cleave the DNA to have sticky ends and not blunt ends. I currently am working on a project ...
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26 views

Alkaline Phosphatase

What is the predominant purpose of Alkaline Phosphatase in skeletal muscle fibers and liver cells. I know that it is a hydrolase enzyme that speeds up the degredation of proteins, lipids, starch and ...
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15 views

How do Streptococcus thermophilus and Lactobacillus bulgaricus precipitate the curd of swiss cheese?

I learned in my food microbiology class that Streptococcus thermophilus and Lactobacillus bulgaricus precipitate the curd of swiss cheese. However, I was wondering what type of mechanisms do these ...
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16 views

fermentation to acetic acid

How many time need for swich fermentation to acetic acid production cycle , and the conversion of apple vinegar in a sealed container incubated at 37 ° C ? Should be in the fully closed? What is ...
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29 views

Are there any species which can live off rocket fuel and cosmic radiation? [closed]

It was recently reported that cosmonauts have found sea plankton on the outside of the space station. Space agencies are nowadays very careful to sterilize their interplanetary landers, and orbiting ...
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63 views

Genetically modified Klebsiella Planticola nearly bulldozers plant life as we know it?

The article "The Bacterium That (Almost) Ate the World" by Elaine Ingham (see also here or here) describes a genetically modified bacterium that would break down cellulose plant matter into alcohol: ...
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41 views

What is Mycoplasma Lyo medium?

Used for instance in this article The role of genital mycoplasmas as pathogens - - are generally neglected by diagnostic laboratories in the United Kingdom, possibly due to the lack of a ...
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31 views

Regulation of Cra protein level in E coli

Catabolite Activator/Repressor, Cra protein (formerly known as Fructure Repressor FruR) plays a significant role in central carbon metabolism of E coli. Its activity is inhibited by ...
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39 views

Vitamin D oral intake, transportation and absorption

Several factors affecting vitamin D and its active form absorption and storage acidity of stomach (not significant effect) cytopathic effects of viruses cytopathic effects of bacteria where the ...
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1answer
31 views

What are the best detection medias for cholera?

I heard this fact that you can use some [hypertriade] for vibrio cholera diagnosis which has compontents sucrose (yellow) mannose (yellow) arabinose (do not ferment; stay dark pink) I did not find ...
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19 views

How are lophotrichous flagella of helicobacter pylori produced?

I am thinking the mechanism is something like first adhesins (mucinase). Howevever, this does not seem to be enough. How are the flagella of H. pylori produced?
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49 views

What are current abiogenesis hypotheses for first food source?

What is the very first abiogenetic piece of reproducing life (small piece of RNA + ribosome that randomly occurred?) hypothesized to have used as an energy source? I'd be interested in sources to what ...
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904 views

Bacterial 16S rRNA PCR amplification with universal primers

I am doing an experiment to amplify the 16S rRNA gene from bacteria present in gut contents of fish. After extracting DNA, I perform one-step PCR with universal bacterial primers (27F, 1492R) and I ...
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55 views

What are the ingredients of Pheromone Trap using for controlling Fruit flies of cucumber?

Pheromone Traps are used for fruit fly control. But I have no idea which ingredients or chemicals are used for preparing Pheromone trap
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24 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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36 views

algae lamp questions?

I would be wondering if you help me if you know about algae lamps please give me detail about it and answer my questions This is my questions? how the algae will charge the battery in day?(process) ...
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66 views

What are some readily available, easy to test, bacteria?

I want to do a science fair experiment about how bacteria interact with 5-carbon vs. 6-carbon sugars, and I want a bacteria that is easy to test and is readily available. Any ideas?
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36 views

What are the characteristic structures of bacillus M. tuberculosis and what they cause?

I answered to this question: In most forms of the disease, the bacillus M. tuberculosis spreads slowly and widely in the lungs, causing the formation of hard nodules (tubercles) in the ...
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19 views

Can antiviral antibodies help viral infections which has many serotypes?

For instance Dengue virus has four serotypes. Infection by a different serotype causes only a more serious infection. 2) Which are the most common virus infections that has many serotypes?
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22 views

Looking for detailed nutrient/energy flow at the bottom of the freshwater food chain

I am trying to model the production and consumption of nutrients and waste at the bottom of the food chain in freshwater ecosystems. I can only find broad information on the Internet and don't know ...
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41 views

Can viral proteins cause infection? [duplicate]

Possible Duplicate: HIV Rapid Tests Can proteins found on the surface of RNA viruses cause infection without the presence of the actual RNA and other components used in the virus?
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49 views

Solid phase use in HIV rapid tests

I have another question in regards to my HIV test research. The rapid tests like Orasures Oraquick contains a strip of synthetic peptides that are used to represent proteins found in the envelope ...
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43 views

Where can I find approximate rates of sequestration of CO₂ for different species of algae?

For a study, I want to compare the rates of CO₂ sequestration and fixation of a few different species of algae. I could not find any data on the sequestration rates. Any pointers to where I can find ...
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52 views

What does this sentence about toxemia and Clostridium tetani mean?

I know that Cl tetani is not invasive and strictly localised. I think toxemia means spread in the blood. I am thinking this sentence The volume of infected tissue is small, and the disease is ...
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18 views

S. pyogenes' skin infection diagnostics

S. pyogenes is "flesh-eating" bacteria. It results from life-theatening myonecrosis caused by this organism. S. pyogenes avoids phagocytosis (mediated primarily by capsule, M and M-like proteins, C5a ...
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2answers
87 views

What is the right spelling for this agar? [closed]

I could not spell the agar [gonoline-uroline] which I heard yesterday. My spelling is so wrong that I could not find it in Google. What is the right spelling for this agar?
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21 views

To understand clearly extraintestinal diseases

Extradistestinal disease seems to prefer to "bowel" diseases, I think this is about diseases outside gastrointestinal tract so stomach. Consider diseases ulcerative colitis and Crohn's disease ...
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2answers
42 views

Is there any format for official Physiological/Medicine answers? [closed]

Assume you have an exam which has 5 extensive questions and 60 minutes. You do not have time to cover most if you write everything in essay format. If you start to write essays, you do not really have ...
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1answer
43 views

Looking for raw cell videos from a light microscope

First off, I'm not looking for online lectures. I am trying to find creative commons licensed videos of live cells moving around without annotation or anything extra. I would prefer a few minutes of ...
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1answer
34 views

How to Diagnose this hepatitis A, B and C case?

Male patient who is working in the hospital and may be infected with contaminated blood had hepatitis with genome IA 5 years ago. His hepatitis A at that time was confirmed with the rise of anti.HAV ...
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1answer
33 views

Shigella's O antigen (PAI synthesised) and passage through host defences

The O antigen is synthesised by Pathology islands (PAI). O antigen may be a factor why Shigella survives the passage through host defences. I am interested in which step of pathogenesis. There are ...
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1answer
36 views

What is the maximum amount of different bacteria in the drinking water in Europe?

I am looking for a statistical amounts which are allowed. Some students say it is 0 for all bacteria, which I think is false. I found this USA source. I found there Total Coliforms (including fecal ...
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1answer
54 views

Difficulty in developing certain vaccines

I have a college level background in Biology, say at the level of Campbell. I am very curious to know why it's extremely difficult to develop vaccines for certain diseases. Two cases which I am really ...
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1answer
21 views

How to get conservation of vaccine candidates between hundreds of bacterial strains?

We have identified vaccine candidates via an ex-vivo RNA-seq approach. Next step would be to perform conservation of these candidates (about 20) between multiple bacterial strains (about 200). I would ...
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47 views

What is it the most common reason for community acquired pneumonia?

This is a difficult question because there are many types of community-acquired pneumonia (CAP): typical acute, atypical acute and chronic. Most common microorganisms are also different among ...
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38 views

Can acid-fast M. tubercolosis obstruct alveoli?

I am not sure which word is the best here for colonisation of alveoli by acid fast-mycobacteria. I found this question, and I am getting confused by the term obstruction of alveoli because I tend to ...
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1answer
33 views

First genomic survey of human skin fungal diversity

"The researchers found that a single type of fungus, belonging to the genus Malassezia, is predominant on the head and trunk. Hands, which harbor a great diversity of bacteria, are home for relatively ...
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1answer
22 views

Does adding antibiotic after 5-10 mins of innoculation affect the protein yield or growth?

I've asked a lab colleague the same question. She said, it would loosen the bacterial cells in the LB medium and plasmids would come out. Is that true? and why?
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Answer the questions in one word with help of clues and images given [closed]

Name the things associated with Antonie Van Leeuwenhoek - (6 pictures are given for help and four of them are associated with these clues) a)Leeuwenhoek was the first to describe these and hence ...