Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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When and How do pacemaker cells develop during the cell aggregation process of Dictyostelium discoideum?

I was reading a paper by Tang & Othmer about oscillations and waves in Dictyostelium discoideum. Under certain condition like starvation period in the life cycle of a Dictyostelium discoideum ...
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What does the term “relay competent” mean?

I was reading the article of Dallon & Othmer (2010) which deals with cell aggregation in Dictyostelium discoideum. In the introduction of the paper it is said that cells becomes "relay competent". ...
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64 views

What is the difference between electrons and energy? [on hold]

I'm studying microbiology right now and I have come across something confusing to me. I thought electrons provided energy to the cell by being incorporated into reducing powers and eventually driving ...
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40 views

Medium for Pseudomonas?

What is a good minimal medium for Pseudomonas bacteria? Do I need different media for different Pseudomonas species?
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82 views

Inoculation vs. vaccination

Is there any actual difference between inoculation and vaccination or are these terms interchangeable? In case the difference exists, would it be correct to say that inoculation is purposefully ...
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What colony is growing on this petri dish? [closed]

I plated some stream water on agar and incubated the dishes at room temperature. I am curious to find out what the red streaks on the agar are. Is it E. Coli? Or is it some sort of fungus...
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75 views

Why do slime molds pulsate when growing into their fruiting body?

An example can be seen in this Youtube video, where the slime mold pulsates as it engulfs a rock in order to form its fruiting body. Why does this pulsing happen?
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34 views

E. coli not growing in liquid medium

We are regularly doing bacterial transformation and subculture from plate to liquid media to extract DNA. This usually goes very well and is straightforward, but occasionally, the colonies that grew ...
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17 views

Can the Staph streak method be used for culturing Neisseria on blood agar?

What I learned in class: Normally chocolate agar is needed to grow Haemophilus and Neisseria. The Staph streak technique can be used for culturing Haemophilus on blood agar by allowing the Staph to ...
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2answers
59 views

What is a good source that will help me classify protists?

I am a 15 year old that enjoys looking at the micro world. The only issue that I have is that I can't find a good online source that will help me classify the name of the micro organism that I'm ...
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Why do flagella form a bundle only when they rotate counterclockwise during chemotaxis?

During Chemotaxis in bacteria with flagella, the flagellar rotation dictates how the cell moves. If the flagella rotate counterclockwise, then they form a bundle at one end of the cell (---O) and ...
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48 views

When do plasmids replicate relative to its host cell cycle?

For plasmids is so much shorter than their host cell's genome (about 1/1000 in my case), it will take only 1/1000 time for it to replicate. With respect to cell cycle, when will that replication ...
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Hot water and bacteria

I know that it is common to say, "use hot water when washing your hands" or when you've got a cut, "wash your hands with warm water," etc. I was wondering, why is this the case? Since bacteria grow in ...
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Why are pili more common in Gram negative bacteria than in Gram positive?

Although pili have been observed in some species of Gram positive bacteria, the preliminary research that I have done indicates that pili are significantly more common in Gram negative bacteria. Is ...
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2answers
91 views

Does freezing microorganisms such as probiotics kill them?

Does freezing microorganisms such as probiotics kill them? If not, what is the process that allows them to "come back to life" after the temperatures are increased? As an example, lets say you ...
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0answers
26 views

The ring formation of ringworm

I know ringworm is caused by a fungus on the skin, nails or scalp but what causes the rash to form as a ring instead of like a normal spread out rash?
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105 views

Non-harmful bacterial invasion of cells

There are bacteria that can enter body cells as parasites. Could it be that some of these are benign, such that the guest will not kill the host cell it lives in (especially in human)?
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149 views

Are there single-celled organisms that have evolved from multi-cellular ones?

I'm reading this paper about transmissible cancer cells in clams (Metzger et al. 2015) and I was wondering if there are any single-cellular organisms that are around today that are suspected as having ...
3
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1answer
26 views

Microalgae without cell walls?

Most microalgae have rigid cell walls. Dunaliella Salina is a pretty famous example of an algae with no cell wall, but just a plasma membrane. Are there any other microalgae without a cell wall?. I ...
3
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1answer
31 views

Regulation of V. cholerae virulence factors

So I know that several different environmental signals, such as pH, bile, and temperature, regulate virulence gene expression in V. cholerae. Specifically, they control expression of the genes ...
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2answers
175 views

HIV Rapid Tests

The hiv antigens that are used in the oral rapid tests, are they infectious? The tests do not contain any actual virus but I am curious if the antigens themselves could somehow create the virus on ...
7
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1answer
49 views

Reasons for the HIV-1 epidemic

So, the HIV-1 jump to humans occurred as early as the 1920s, but the AIDS epidemic didn't start until the early 1980s. Some things I don't understand about this: Why the delay? What is needed for a ...
2
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1answer
50 views

Horizontal Gene Transfer

I understand the different ways bacteria can undergo horizontal gene transfer (transformation, transduction (phages), conjugation (plasmids)). Is there an experimental method to tell how a specific ...
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1answer
18 views

Ways to cause membrane damage to microalgae and yeast?

I am researching a way to monitor the membrane damage of cells. To do that I fist have to have reference points, namely, cells with damaged membranes. I am working with Dunalliela, Hematococcus (both ...
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1answer
47 views

Ethanol production by fermentation?

As bacteria are involved in the production of ethanol through fermentation and ethanol is also used as antiseptic that kills bacteria, so how and why bacteria are involved in the synthesis of such ...
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Are there mechanisms that limit the amounts of time conjugation and F-plasmid transfer can happen? If yes, what are they?

Not all bacteria have acquired F-plasmids through conjugation. Some of the mechanisms for this are unsuccesful conjugation events (mechanical disruption), no transfer due to integration in bacterial ...
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1answer
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Why don't all bacteria have F-plasmids by now?

Some bacteria can undergo gene transfer by conjugation. Conjugation is a form of horizontal gene transfer, meaning from one (unrelated) bacterium to another (in contrast to vertical gene transfer, ...
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Why can't some organisms match miRNA perfectly to the target mRNA like in plants? [closed]

What causes other organisms to be impaired in making perfect matches like plants do and is there a way to increase matching?
6
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1answer
134 views

What allows Valonia ventricosa cells to get so big?

Valonia ventricosa are single celled algae that range between one and few centimeters. In rare cases they can reach sizes exceeding 5cm. Weirdly, a lot of the literature covering these organisms ...
6
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1answer
117 views

Why are there no known photosynthetic archaea?

I'm taking a microbial physiology course and we noted that, while some archaea are phototrophic, there are no known photosynethetic archaea. Are there any physiological characteristics that make ...
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2answers
55 views

What happens during kefir fermentation process?

I’ve found many sources about the positive effects of kefir for the digestive system. However I haven’t found any information about the fermenting process. What is the exact biology (chemistry?) ...
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Why is acid alcohol used as a decorizer in the acid fast stain procedure, as opposed to a neutral alcohol?

Is it to neutralize the negative components of the cell membrane, thus preventing the positively charged dye from adhering to the cell membrane?
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1answer
59 views

To which negatively charged components of the cell envelopes do the crystal violet complexes bind in gram staining?

The gram positive have negative components in the peptidoglycan layer in the form of teichoic acid phosphodiester bonds, and the gram negative have negative components in their outer membrane in the ...
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1answer
65 views

Survival of streptococcus after my scarlet fever

I'm currently sick because of scarlet fever but I got treated with antibiotics. I know that after 1.5 day using antibiotics the streptococci I release at home are dangerous anymore. But my question ...
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174 views

What is this fuzzy, black fungus that grew on my plates in the 4⁰C room? [closed]

I often find the fungus below growing on my (ostensibly) sterile plates in the 4⁰C room. Presumably it takes a few days to reach this size. The colony looks puffy and dimpled in the middle, like a ...
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1answer
38 views

Why are plasmid genes not already incorporated into bacterial chromosomes if necessary for stressful situations?

If plasmids are important for bacteria to express specific genes under stressful conditions, why are these genes not already incorporated into their chromosome to begin with? What is the evolutionary ...
2
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1answer
73 views

How do Streptococcus thermophilus and Lactobacillus bulgaricus precipitate the curd of swiss cheese?

I learned in my food microbiology class that Streptococcus thermophilus and Lactobacillus bulgaricus precipitate the curd of swiss cheese. However, I was wondering what type of mechanisms do these ...
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8 views

Reasons preirradiation of negatively stained and plastic embedded specimens with a low electron dose improve stability to electron irradiation

It is known that preirradiation of negatively stained and plastic embedded specimens with a low electron dose improves their stability to electron irradiation. But my question is why this occurs? ...
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Clumping factor test

I'm come across this question and I'm somewhat confused: In the test for staphylococci, the clumping factor detects: staphylothrombin DNase mannitol fermentation Fimbriae fibrinogen-binding protein ...
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2answers
138 views

Growing E. coli at room temperature?

If I were to do a blue/white selection of transformed E. coli on LB agar ampicillin plates at room temperature (23⁰C) for about 2 days and 18 hours, will I run into the issue of satellite colonies or ...
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1answer
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36 views

What Websites Have Image Libraries for Bacteria and other Microorganisms

What Websites Have Image Libraries for Bacteria and other Microorganisms? With age of cell phone microscopes and hand held spectrometers it would be interesting and valuable to be able to compare ...
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1answer
66 views

How often does bacterial transformation happen?

I have been reading: M. Dröge, A. Pühler, W. Selbitschka, "Horizontal gene transfer among bacteria in terrestrial and aquatic habitats as assessed by microcosm and field studies", Biol. Fertil. ...
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3answers
151 views

If someone were to die on the moon, would their body decay?

I heard that the footprints of Neil Armstrong are still there, so I was wondering if someone were to die there, would they remain preserved, too? If not how long would it take for them to decay?
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1answer
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The damage of cancer cells

I read about the molecular biology of cancer, and I have a mess on my head and a lot of questions.. . My primary question is- The damage of the cancer cells is in the dna sequence or in the gene ...
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22 views

Are there any viruses that are part of all land animals?

An article on I Fucking Love Science (linked to below) got me thinking, are there any viruses that have been so successful that they have spread to all land animals similar to Toxoplasmosis which has ...
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1answer
66 views

Is there any way the food industry can benefit from biofilms?

I realize all of the disadvantages, but I am wondering if the food industry can actually benefit from the formation of biofilms.
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1answer
74 views

Molecular Cloning- Blunt end restriction endonucleases

I work in a microbiology lab where we do a lot of cloning. I have always used restriction endonucleases to cleave the DNA to have sticky ends and not blunt ends. I currently am working on a project ...
7
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1answer
117 views

How many eukaryotes are there on Earth?

I have been reading: William B. Whitman, David C. Coleman, and William J. Wiebe, "Prokaryotes: The unseen majority", Proc. Natl. Acad. Sci. USA 95, pp. 6578–6583, June 1998. [Full Text] [PDF] ...
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What are some places where biofilms could develop? [closed]

I'm trying to think of places where a biofilm could develop other than on medical equipment or food processing equipment such as stainless steel mechanized blades or knives. I'm thinking more along ...