Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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What is a good source that will help me classify protists?

I am a 15 year old that enjoys looking at the micro world. The only issue that I have is that I can't find a good online source that will help me classify the name of the micro organism that I'm ...
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1answer
49 views

Reasons for the HIV-1 epidemic

So, the HIV-1 jump to humans occurred as early as the 1920s, but the AIDS epidemic didn't start until the early 1980s. Some things I don't understand about this: Why the delay? What is needed for a ...
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14 views

Spatial learning in microorganisms

Has there ever been an experiment performed that demonstrated a form of 'spatial memory' in a unicellular organism? I'm imagining something analogous to the classic 'rat in maze' experiments, but ...
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0answers
43 views

When and How do pacemaker cells develop during the cell aggregation process of Dictyostelium discoideum?

I was reading a paper by Tang & Othmer about oscillations and waves in Dictyostelium discoideum. Under certain condition like starvation period in the life cycle of a Dictyostelium discoideum ...
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69 views

E. coli not growing in liquid medium

We are regularly doing bacterial transformation and subculture from plate to liquid media to extract DNA. This usually goes very well and is straightforward, but occasionally, the colonies that grew ...
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0answers
20 views

Why do flagella form a bundle only when they rotate counterclockwise during chemotaxis?

During Chemotaxis in bacteria with flagella, the flagellar rotation dictates how the cell moves. If the flagella rotate counterclockwise, then they form a bundle at one end of the cell (---O) and ...
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0answers
235 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
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0answers
51 views

How can I keep pond water “alive”?

I've been preparing wet slides for my kids to look at from pond water. I have a mason jar of pond water. What can I do to keep the microbes in the jar alive going forward so that we can look at it any ...
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0answers
51 views

CRISPR-Cas Systems

In the context of the bacterial systems (not the gene editing tool), I was wondering what happens to the foreign DNA after the Cas proteins have created a new spacer. It is really not clear to me, ...
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0answers
28 views

What is effectiveness of methyl-alcohol as sporicide?

Methyl-alcohol is the weakest alcohol in terms of bactericidal properties. However, I have read that it also works as sporicide. What is the effectiveness of methyl-alcohol in killing the spores of ...
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0answers
35 views

The ring formation of ringworm

I know ringworm is caused by a fungus on the skin, nails or scalp but what causes the rash to form as a ring instead of like a normal spread out rash?
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0answers
69 views

What are the ingredients of Pheromone Trap using for controlling Fruit flies of cucumber?

Pheromone Traps are used for fruit fly control. But I have no idea which ingredients or chemicals are used for preparing Pheromone trap
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0answers
263 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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0answers
37 views

Elevated transaminases after blood transfusion?

This sparks HCV immediately in my mind. However, there may be other possibilities too. What can you deduce from elevated transaminases if you only know that the healthy adult patient received blood ...
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0answers
45 views

Endophytic Xylariaceae: diversity and taxonomy inferred from rDNA sequence analyses

"Thailand is considered as one of the areas containing a high percentage of unknown taxa of Xylariaceae (Rogers 2000). In Thailand, several studies on endophytic fungi have been documented, namely, ...
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0answers
38 views

How is the appropriately-stratified gut microbiome acquired in organisms performing horizontal transmission?

I am studying horizontal transmission of primary symbionts in insect reproduction. This reminded me of an earlier question I had asked, in which I learned that humans analogously acquired their ...
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12 views

Clumping factor test

I'm come across this question and I'm somewhat confused: In the test for staphylococci, the clumping factor detects: staphylothrombin DNase mannitol fermentation Fimbriae fibrinogen-binding protein ...
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0answers
18 views

What are the main principles of the Bacteriological Code?

Although the Bacteriological Code is an important guideline for taxonomists and microbiologists, describing the criteria for delineating species and higher taxa of the prokaryotes, I, and plenty of ...
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0answers
59 views

Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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0answers
42 views

Alkaline Phosphatase

What is the predominant purpose of Alkaline Phosphatase in skeletal muscle fibers and liver cells. I know that it is a hydrolase enzyme that speeds up the degredation of proteins, lipids, starch and ...
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0answers
16 views

Switch gut microbiota due to diet and effect on calorie uptake

I have seen peer-reviewed papers mentioning the daily changes in gut microbiota composition according to dietary changes. See for example this paper: http://genomebiology.com/2014/15/7/R89 My ...
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0answers
79 views

What is Mycoplasma Lyo medium?

Used for instance in this article The role of genital mycoplasmas as pathogens - - are generally neglected by diagnostic laboratories in the United Kingdom, possibly due to the lack of a ...
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0answers
67 views

Regulation of Cra protein level in E coli

Catabolite Activator/Repressor, Cra protein (formerly known as Fructure Repressor FruR) plays a significant role in central carbon metabolism of E coli. Its activity is inhibited by ...
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49 views

Vitamin D oral intake, transportation and absorption

Several factors affecting vitamin D and its active form absorption and storage acidity of stomach (not significant effect) cytopathic effects of viruses cytopathic effects of bacteria where the ...
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26 views

How are lophotrichous flagella of helicobacter pylori produced?

I am thinking the mechanism is something like first adhesins (mucinase). Howevever, this does not seem to be enough. How are the flagella of H. pylori produced?
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0answers
68 views

What are current abiogenesis hypotheses for first food source?

What is the very first abiogenetic piece of reproducing life (small piece of RNA + ribosome that randomly occurred?) hypothesized to have used as an energy source? I'd be interested in sources to what ...
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0answers
1k views

Bacterial 16S rRNA PCR amplification with universal primers

I am doing an experiment to amplify the 16S rRNA gene from bacteria present in gut contents of fish. After extracting DNA, I perform one-step PCR with universal bacterial primers (27F, 1492R) and I ...
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40 views

Improving throughput of CFU plating?

In a separate question I've described my general experimental setup where I need to measure the number of live cells in a growing bacterial culture in a fairly rapid and high-throughput manner. In ...
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0answers
44 views

What are the characteristic structures of bacillus M. tuberculosis and what they cause?

I answered to this question: In most forms of the disease, the bacillus M. tuberculosis spreads slowly and widely in the lungs, causing the formation of hard nodules (tubercles) in the ...
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0answers
22 views

Why is acid alcohol used as a decorizer in the acid fast stain procedure, as opposed to a neutral alcohol?

Is it to neutralize the negative components of the cell membrane, thus preventing the positively charged dye from adhering to the cell membrane?
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0answers
8 views

Reasons preirradiation of negatively stained and plastic embedded specimens with a low electron dose improve stability to electron irradiation

It is known that preirradiation of negatively stained and plastic embedded specimens with a low electron dose improves their stability to electron irradiation. But my question is why this occurs? ...
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0answers
23 views

Are there any viruses that are part of all land animals?

An article on I Fucking Love Science (linked to below) got me thinking, are there any viruses that have been so successful that they have spread to all land animals similar to Toxoplasmosis which has ...
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0answers
20 views

What's the composition of Actinobacteria's hyphae?

I'd like to know which chemical compounds form hyphae in Actinobacteria. Is it the same structure of the cell wall? Thank you