Microbiology is the study of organisms that are too small to be seen with the naked eye. This includes organisms like bacteria, fungi, protozoa, and others.

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If a human takes antibiotics are all bacteria in the body killed?

From my basic understanding, antibiotics kill living things, bacteria for example. Do the antibiotics consumed by a human-being distinguish between what they kill? Or do they just kill every bacteria ...
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4answers
979 views

How to understand influenza strain designations?

What do the strain designations for flu mean? For example avian flu is classified as H5N1, what do the letters H, ...
17
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309 views

How does the microbial environment in your gut initiate?

Clearly, a zygote does not harbor any microbes. As it develops, and the alimentary canal tissue is differentiated, I logically assume that there is still no microbial activity in the fetus's gut. I'm ...
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1answer
228 views

Is there any convincing evidence for the existence of nanobacteria?

The existence of nanometer scale microorganisms has been proposed and used explain several phenomena including morphological structures in a martian meteorite (ALH 84001) and implication in the ...
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3answers
686 views

Are single-celled organisms capable of learning?

I've read that the amoeba is capable of learning. Since these protists have no nervous system, it's safe to assume that even highly simplified learning mechanisms of the Aplysia are miles off in the ...
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183 views

Why is there now only one Salmonella species?

Once upon a time, I chanced upon an old microbiology book that detailed the rather colorful world of enterobacteria. Salmonella in particular stood out, as it seemed there were a lot of species: typhi ...
14
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1answer
129 views

Are there animal models for Clostridium difficile that better replicate human infection than hamsters?

So I'm looking for some information on the infectious dose necessary to colonize a human with Clostridium difficile. There's no human challenge studies, and since it's not a foodborne pathogen, little ...
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168 views

Why is the microbial ecosystem of the gut so susceptible to disruption by pathogens?

From all accounts, it seems as if the Escherichia, Enterobacter, etc. that live and thrive in the human gut are pretty well entrenched. I know that these microbial populations are often analyzed as ...
12
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128 views

Relationship between our microbiome and personalized nutrition

Recently, it has been asked whether there are 'metabolic types' between humans that can benefit from a sort of personalized nutrition. One answer suggested that one discerning factor could be the ...
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571 views

Do probiotics survive digestion?

Pretty much this. I've been wondering if any of the yogourt and other "health" foods containing living probiotic cultures survive digestion to populate our intestines? If so, is there peer-reviewed ...
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2k views

What is the advantage of using starter cultures for growing bacteria?

Many DNA isolation and protein expression protocols contain instructions to use a starter culture of E. coli that is then used to inoculate the main culture. What are the advantages of using starter ...
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111 views

How do baby animals that primarily subsist on cellulose get their initial gut flora?

In the case of mammals like giraffes and koalas, is that bacteria common on the plants they eat so when a baby starts to try to stick something besides its mother's milk in its mouth, it can't digest ...
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137 views

Is there an equivalent to “Fields Virology” for Bacteria?

I've gotten a staggering amount of use out of my copy of Fields Virology as a general reference for "getting me up to speed" on whatever pathogen I'm currently looking at. I don't know of a similar ...
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415 views

Antibacterial hand soaps (and related products); what are they good for?

From Wikipedia: Household use of antibacterials in soaps and other products, although not clearly contributing to resistance, is also discouraged (as not being effective at infection control). ...
10
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1answer
127 views

Are these Gram stain substitutions acceptable?

In the context of a Gram stain on a blood smear: Are the following acceptable substitutions and/or what differences could arise by substituting them? Using methylene blue instead of crystal violet ...
9
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1answer
240 views

Can Naegleria fowleri enter through wounds into the bloodstream?

All the sources I've read said that Naegleria fowleri enters the brain through the nose. But lets say that someone had cuts on their arm or leg and they are swimming in water that is conducive to ...
9
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1answer
110 views

Have there been any positive public health effects due to UV lights?

Occasionally, in hospitals and in eating establishments in the US, they have industrial grade UV lights in sconces attached to the wall (though they seem to be less prominent as the years go by). I ...
8
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2answers
424 views

How much weight/volume do microbes occupy within the human body?

Microorganisms constitute the bulk of all the biomass on Earth. I weighed myself yesterday, and wondered how much less I would weigh if I were completely free of bacteria and microbes, inside and out. ...
8
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1answer
159 views

Is it plausible that eukaryotic organelles like flagella and cilia are the result of endosymbiosis with spirochetes?

This was a claim by Lynn Margulis explained over at this link. The sense organs of vertebrates have modified cilia: The rods and cone cells of the eye have cilia, and the balance organ in the ...
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2answers
541 views

Bacterial cell lysis - what solution to use?

I am trying to determine how quickly detergents act on bacterial cells (cell lysis). I would like to compare some detergents at difference concentrations for bacteriolytic activity. I don’t care about ...
7
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2answers
75 views

Should we be looking for extra-terrestrial life on comets?

I have just read What elements are a possible basis for life? and I find myself wondering whether instead of seeking advanced life-forms at remote locales a La SETI, and perhaps fundamentally ...
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1answer
99 views

Any food crops that could grow in a desert provided that they get fertilizer and water

I am working on an idea to provide nearly unlimited amounts of water in desert regions. To make this commercially viable. I would use the water to irrigate desert sand and grow crops. Given my ...
7
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1answer
266 views

How is the exogenous DNA protected from degradation during bacterial transformation?

During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
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644 views

What's the aim of genetically modifying of foods/organisms?

On news, articles etc. experts talking about Genetically Modified Foods and Organisms often mentions about their disadvantages like, their potential to harm human health allergies may become more ...
6
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1answer
197 views

Models of quorum sensing for multi-agent systems

Quorum sensing is a system of stimulus and responses correlated to population density that is used by bacteria to coordinate gene-expression. I am looking for a simple computational/mathematical model ...
6
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1answer
308 views

If re-boiling does not curdle the milk, can that be taken as an indicator that the milk hasn't developed the bacteria (and their toxins)?

Usually when left in hot temperature, the milk curdles (which I know when I boil it and it starts separating). If the re boiling does not curdle the milk, can that be taken as an indicator that the ...
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135 views

HIV Rapid Tests

The hiv antigens that are used in the oral rapid tests, are they infectious? The tests do not contain any actual virus but I am curious if the antigens themselves could somehow create the virus on ...
5
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137 views

Can genetically modified genes jump to bacteria in the eater's intestine?

The Guardian ran an article a while back talking about GM gene's jumping to bacteria in an eater's intestine. Has other research confirmed this phenomenon?
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314 views

What does the 34/70 in Saccharomyces pastorianus Weihenstephan 34/70 stand for?

I've searched everywhere. No Wikipedia page. No information on NCBI. I searched all occurrences of 34/70 in some primary research articles! The best I've found is this brewery forum where someone ...
5
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1answer
93 views

What types of archaea have been found in animals?

I am curious as to the species or "types" of archaea that have been found to reside within animals symbiotically. One of the only ones I can think of off the top of my head are methanogens, which live ...
5
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1answer
946 views

What is the distinction between F' plasmid and R plasmid?

Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...
5
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1answer
39 views

Is a sequential double transformation acceptable?

Standard protocol states having two compatible vectors being transformed simultaneously during the same procedure. I've come across a situation in which transforming one vector, obtaining results, and ...
5
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1answer
146 views

Alternatives to CFU plating for measuring number of viable cells?

I am hoping to measure growth rates of a bacterial culture in several growth conditions. I am concerned that these growth conditions may cause cell death, which would lead to a decreased ...
5
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1answer
100 views

Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?

I'm currently working on a synthetic biology project which involves working with lots of different parts. I would ultimately like to integrate these genes by transforming a single plasmid. I've heard ...
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Why does milk contained in cartons of milk expand?

In the morning, I went to the fridge to drink some chocolate milk. At night, when I took out the same carton of milk, the packaging seems expanded, like some kind of air is inside. Explain please !
5
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810 views

Why are pili more common in Gram negative bacteria than in Gram positive?

Although pili have been observed in some species of Gram positive bacteria, the preliminary research that I have done indicates that pili are significantly more common in Gram negative bacteria. Is ...
5
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2answers
296 views

Survival of E. coli on agar plate at -20 degree C without glycerol stock

If I kept E. coli transformed with plasmid at -20˚C without making glycerol stock, will it survive? Actually I have kept it this way for 18 hours. What will happen with it? Will it grow in fresh ...
5
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1answer
265 views

Aren't antibiotic resistant probiotics dangerous?

Multidrug resistant probiotics are often recommended by doctors in various cases. But since bacteriae can easily exchange genes by conjugation or other means they could promote the drug resistance of ...
5
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1answer
109 views

Mutation-immunity in Luria-Delbruck experiment

If experiments like those of Luria and Delbruck on E. coli and T1 phage are the main source of our confidence in the mutation-immunity model, is it then highly unlikely that there are other types of ...
5
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2answers
310 views

Sparking during electroporation of plasmid DNA into bacterial cells

During electroporation of bacterial cells (I work with Mycobacterium tuberculosis, but I think this applies to E. coli as well), sometimes I get sparking. I've read this is due to salts present, ...
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475 views

Why do yeast ferment Equal sweetener

I had my students conduct a fermentation experiment manipulating sugar amounts (number of sugar packets in solution) and measuring CO2 production via capture in a balloon and measuring circumference ...
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115 views

Are all known germs benign in small enough quantities? i.e. Why do we wash our hands?

We know that we wash our hands because germs can get into our body through the nose, the mouth, the eyes, cuts, etc. But I doubt we can completely clean our hands of germs every time we wash them, ...
4
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1answer
71 views

Are there any bacteria, in our environment, able to break down nitrate under aerobic conditions?

Given a fix volume of water saturated with dissolved oxygen through aeration. Suppose that ammonium, Nitrosomonas, and Nitrobacter are always available in the water. We know that some of the ammonium ...
4
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2answers
337 views

Does making yogurt from non-pasteurized milk work against possible disease bacteria?

In the past, when there was no pasteurization, could making yogurt from milk lower the chance of getting infected by bovine tuberculosis (or other diseases from infected milk)? For example, would ...
4
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1answer
23 views

Is the regulation of lactose operon different between Gram + and Gram -?

I know that in E. coli the lactose operon is shut down by CAP protein when binding cAMP. Is this true also for Gram positive bacteria?
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142 views

Is every part of a virus important for replication?

Is every component of a virus absolutely essential for its infection and replication in a host cell? Or can you just have parts of it to cause infection?
4
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1answer
134 views

Non-monotonic knock-out effects in prokaryotes

Typically, when performing gene-knockout, the experimenters select one gene to remove/replace-with-junk and then see if the prokaryote can still undergo fission. If it continues to reproduce then the ...
4
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1answer
54 views

Need for proper air filter for bioreactor?

I am going to build a simple bioreactor for microalgae growth. I already have fluid filters pore size: 0.2um, diameter: 25mm https://nl.vwr.com/app/catalog/Product?article_number=514-4039 I wonder ...
4
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1answer
131 views

Vigorous shaking for HFR interrupted mating

I am trying to reproduce E. coli interrupted mating using an HFR strain, and I read that the cultures should be vigorously shaken at times to interrupt the DNA transfer from the F+(HFR) donors to the ...
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1answer
365 views

How to obtain bacteria samples at home?

As the original question went from hold to closed, I thought I would write up a more appropriate question. How should one go about getting bacterial samples to look at under a microscope at home? ...