The technique of using microscopes to view objects that are otherwise too small to be seen by the naked eye.
2
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0answers
62 views
Software for counting fly eggs
Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid ...
4
votes
1answer
48 views
What is a simple protocol for staining cells in suspension?
I am an engineering student studying how electric fields affect cells, specifically the phenomena of electroporation in living cells.
I know that electroporation is widely used for introducing genes ...
3
votes
2answers
68 views
What kind of microscope should be used to view biological structures (such as spores) roughly 5 µm in length?
I would like to be able to identify genera of fungi based on the shape, size, and colour of spores. So the requirements are:
Must be able to view a 5 micrometre object clearly with reasonable ...
3
votes
0answers
43 views
Microscopy Book Suggestions
I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
votes
1answer
31 views
How to evaluate the efficacy of an antibody for fluorescence microscopy?
I'm trying to evaluate data taken from fluorescence microscopy with antibody staining, and am wondering whether there is any standard way to evaluate the specificity of the antibody for such ...
5
votes
2answers
63 views
What is the state of automated quantitative analysis?
Quantitative analysis of microscope images is essential for many types of studies, but the techniques used most often are very labor-intensive. I have been able to find some literature about using ...
3
votes
1answer
33 views
Atomic force microscopy: depth penetration?
What depth penetration can one typically get with atomic force microscopes? (i.e. how far into the tissue can one image?). I have found numbers on acquisition times (minutes) and spatial resolutions ...
3
votes
3answers
72 views
Super-resolution imaging in vivo
Are there any microscopy modalities or techniques for in vivo imaging at higher resolutions than the diffraction limit?
I was looking at this list: Super resolution microscopy, but they don't talk ...
3
votes
0answers
16 views
Lateral, axional and temporal resolutions for imaging in vivo
What are the spatial (lateral and axional), and temporal resolutions that one is expected to get with the following optical imaging techniques?
Optical Bioluminiscence Imaging (BLI)
Optical ...
2
votes
0answers
75 views
What to look for when buying a light microscope?
I am a science lover and want to buy a microscope to explore things around me. Like studying cell structures, microorganisms, blood and plants. It would be great if there is way to photograph slides. ...
3
votes
0answers
88 views
Why does vision go blurry after microscope use, and how to prevent it?
After using a binocular microscope recently for about 20 minutes, my vision went blurry and for an hour or so it was much harder to read small text. This occurs to a (far) lesser extent whenever I use ...
12
votes
4answers
184 views
Online repositories of scanning electron microscope photographs?
I have looked at every single Wikimedia commons photo in the category 'scanning electron microscope'. Unfortunately, I could not find any other repositories of such photos, but I would readily look at ...
