The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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104 views

How can I keep pond water “alive”?

I've been preparing wet slides for my kids to look at from pond water. I have a mason jar of pond water. What can I do to keep the microbes in the jar alive going forward so that we can look at it any ...
6
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3answers
108 views

What is a good source that will help me classify protists?

I am a 15 year old that enjoys looking at the micro world. The only issue that I have is that I can't find a good online source that will help me classify the name of the micro organism that I'm ...
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0answers
42 views

For observing the cell which objective lens (10x or 40x) should be used with eyepiece lens 10x. And why?

For observing the onion cell which objective lens (10x or 40x) should be used with eyepiece lens 10x, in order to observe the onion cell. And why? Thanks for your help.
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5answers
58 views

Woodland outdoor microscopy advice

Let me start by saying I have never used a microscope outdoors, and in general I have never owned a microscope nor used one regularly. However, I am currently developing and organising some ...
3
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1answer
89 views

Deducing synaptic strength from electron micrographs?

There are several promising techniques for connectomics based on iterative sectioning and imaging of tissue with scanning electron microscopes (e.g. FIB-SEM and ATUM) By looking at such micrographs, ...
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1answer
53 views

Examining milk for presence of harmful bacteria

I am not experienced in diagnostic bacteriology and am interested in understanding more about it in the context of analyzing food purity. For example, imagine I have a supply of raw milk which might ...
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1answer
56 views

Genomic instability of cancer cells in h&e image

Is there are any books or papers on the topic of visual differences(analyzing H&E microscopy) of high grade cancer cells vs low grade cancer cells vs non-cancer cells? In particular, I am trying ...
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0answers
144 views

Is Zeiss Primo Start microscope worth the price for student use?

I study physics but I have got interested about biology too. Surfing around the web, I have found out that in buying microscopes one needs to decide between the cheaper no-name brands vs. the four big ...
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2answers
95 views

Calibrating Motic Camera to Stage Micrometer

Whilst calibrating my Motic Microsope's camera, I am able to calibrate the 10x object lens against the stage micrometer accurately. However when I calibrate (using the Motic 2.0 Plus software) the ...
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1answer
58 views

Why are bacterial cultures necessary?

I notice that in a lot of bacteria testing procedures the sample has to be cultured. You place the sample in agar or another growth medium and wait for the bacteria to proliferate before examining ...
3
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1answer
87 views

Needle-like crystals observed in wild-grape wine under microscope. Potentially harmful?

I recently crushed and strained about thirty pounds of wild grapes to ferment into wine. About a month in, I did a visual inspection of the wine under microscope to try and identify some yeast and ...
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0answers
22 views

Effects of Acids and Bases on protozoa

Why is it that when you pour acid (HCl) on a culture of Euglena gracilis in an aqueous environment the protozoa begins to swell and when you pour a base (NaOH), it maintains its shape? I feel like ...
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15 views

Counting of surface bacteria in real time

I'm fairly new to microbiology (okay, I'm forced to look into it for a group project). What I'm looking for is a way to quantitatively get an estimate of the number of microbes (bacteria and/or ...
3
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2answers
2k views

What magnification do I need to see blood cells?

If I want to buy a microscope for my kids to be able to view single celled creatures and blood cells, about what magnification is required? A Celestron Pentaview digital scope claims up to 600×. Is ...
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0answers
39 views

Effects of Fixation on Cell Membrane

Why does fixing of cells with something like formaldehyde disrupt the membrane enough for probes, antibodies, and dyes to get into the cell?
2
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1answer
35 views

Binocular microscope use, diaphragm?

I am try to review my scope work, since my proffesor asked me to calibrate all the scopes for an intro class in biology, and it has been awhile since I used one. I googled "Binocular microscope use" ...
5
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1answer
120 views

Mites on ginger root

I was just looking at some mouldy ginger found in the fridge under the microscope and was horrified/delighted to find (what looked to be) mites growing in it. I have searched the web and can only find ...
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1answer
195 views

Calculation of final magnification when using a CCD camera

I'm trying to empirically calculate the approx final magnification (mag) of digital images rendered through our confocal microscope. Using a micrometer and the microscopy equipment I have, the ...
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1answer
54 views

Where to find gene manipulation video (or photos)

I hope Im not asking a dumb question! Where can i find video of gene manipulation in action under microscope? Like extracting a gene from a cell, fixing gene mutations, putting the gene in a vector, ...
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2answers
56 views

Dyes to bind to double stranded DNA?

Are there any commercially available fluorescent dyes that will bind only to double stranded DNA (not RNA, single stranded DNA etc.) for studying in vitro using confocal microscopy?
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2answers
281 views

Microscope scale bar

In some microscopic images, there is a bar scale at one of the corners or near a specific object. How are they added to the images? Does the slide have some markers, or are they digitally added? If ...
13
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1answer
694 views

What is the magnification necessary to see pond life

My daughter is 5 and wants to learn about Biology. I thought a great start is to see life from pond water through a USB microscope. It would be great if she could see organisms like amoebas, ...
4
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1answer
78 views

How can I reduce the background gradient in phase contrast microscopy?

I'm looking for ways to set up a phase contrast microscope to reduce the brightness gradient that appears across the background of my images. I use phase contrast to find the outlines of cells in my ...
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0answers
27 views

How to increase contrast with only a top entry specimen holder

My question is: If my microscope had a top entry specimen holder, instead of a side entry holder, what additional option(s) should I have to increase the contrast with the same constraint that I only ...
2
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1answer
471 views

Does methanol fixation deform cultured cells?

I use methanol fixation (@ -20⁰C for 10 minutes) when performing immunofluorescence assays on cultured cells. Generally speaking, this results in very good antibody staining. However, the cells tend ...
4
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1answer
95 views

What microscope/magnification would I need to observe P. Acnes bacteria?

I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
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0answers
27 views

background extraction in microscope analysis

I have taken microscope pictures for proteins in HeLa cells, in order to quantify the intensity (immunofluorescence). The proteins are both at the nuclear envelope, and in order to quantify the ...
5
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1answer
62 views

What Websites Have Image Libraries for Bacteria and other Microorganisms

What Websites Have Image Libraries for Bacteria and other Microorganisms? With age of cell phone microscopes and hand held spectrometers it would be interesting and valuable to be able to compare ...
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4answers
333 views

What kind of microscope for ML/biological research?

I am a computer science student, focusing on machine learning applications. I have been always interested in biology but I lack any training in it. Now, I had an idea that I could introduce myself to ...
4
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1answer
382 views

Software for counting fly eggs

Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid ...
4
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2answers
292 views

How to visualize the ECM?

Specifically, I'd like to look at changes in HA (hyaluronic acid) production. Most often you only see people staining the cell surface or removing cells from culture for fixation and then imaging. ...
2
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1answer
31 views

Can inverted microscope also be phase contrast microscope?

I've read somewhere that inverted microscope also allows phase contrast microscopy. Is that true? Do all inverted microscope have this ability? Do they have any other functions? Many thanks in ...
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1answer
284 views

Can one use a hemocytometer on a compound upright microscope?

I'm posting this as a follow-up on What to look for when buying a light microscope?. The answerer states that you would need to use a an inverted microscope to count cell in the hemocytometer(counting ...
2
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1answer
63 views

What are the features on a microscope one needs in order to do lab work?

By lab work I mean urinalysis, blood work(live as well), fecals, cytologies, histologies and all other. I have read(partly) a book(from 2002) on lab diagnostics and the author did not mention ...
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0answers
25 views

Slicing cell cultures frozen in vitrified ice with a laser for CryoEM

Could a cell culture frozen in vitrified ice for CryoEM be effectively sliced into thin planes and resolved images produced? I was thinking if this could be done to get images from inside a cell.
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2answers
229 views

What to look for when buying a light microscope?

I am a science lover and want to buy a microscope to explore things around me. Like studying cell structures, microorganisms, blood and plants. It would be great if there is way to photograph slides. ...
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1answer
128 views

Can you find malaria parasites in saliva?

Can you find plasmodium parasites (malaria) in saliva under microscope from someone who's infected? Or it's only in the blood?
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4answers
456 views

Machine learning for light microscopy — problems to solve?

I would like solve some biological problems that would improve the state-of-the-art of biology or bioinformatics. In particular, I want to apply machine learning on light microscopic images. The ...
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0answers
101 views

Are there any drawbacks to having a fixed-Köhler microscope?

I am considering to buy the Zeiss Primo star microscope. There are two variants: one with so called "Full Köhler" illumination and one with "Fixed-Köhler" illumination. Are there any drawbacks to ...
2
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2answers
50 views

How much are phase-contrast and dark-field microscopy used in biological research? [closed]

How much are phase-contrast and dark-field microscopy used in biological research today? It seems these were invented a long time ago, so I am thinking would it be a good idea to learn these ...
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1answer
85 views

What are the dimensions in angstrom of bacteria? [closed]

I'm going to take a picture of a bacteria but I am unsure how large it is. What are the dimensions in angstrom of bacteria?
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1answer
105 views

Dataset of microscopic images before and after staining?

I am looking for a freely distributed dataset of microscopic images. I am hoping to find a dataset that would feature histological images before and after staining (e.g. H&E or some other standard ...
3
votes
1answer
709 views

Is it true that viruses cannot be seen with phase-contrast microscopy?

There appears to be a lot of material on the internet claiming that viruses can be only seen with electron microscopes, and not with light microscopes. To the contrary, for example this old paper ...
2
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0answers
88 views

How are new staining protocols designed for microscopy?

How are new staining protocols designed for microscopy? Is it manual work or does it rely on computer simulations? Say for example I thought the H&E staining does not bring enough contrast. How ...
4
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1answer
128 views

How to operate a microtome?

In the picture above (Source: Amazon.com, United Scientific MCT001 Hand Microtome) we can see a microtome. How is it operated?
2
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1answer
278 views

Any difference between inverted microscope objective vs normal microscope objective?

I'm planning to build an inverted microscope and I am not sure if I can use a plane achromatic objective of a normal microscope for the inverted microscope as well. Are there any specific needs for ...
2
votes
2answers
137 views

Image analysis of GFP-tagged protein localization bursts

I'm reading an article (full text here) that analyze the dynamics of localization of a GFP-tagged transcription factor (Crz1) over the time at the single-cell level, by taking movies in a fluorescent ...
2
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0answers
363 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
3
votes
1answer
266 views

Can oil be used instead of water in preparing wet mounts for microscopy?

Is there a reason that oil (e.g., vegetable or clear mineral) is not used to make wet mounts in microscopy? I am not referring to oil immersion technique but substituting oil for water in wet mounts ...
2
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1answer
46 views

What's the best way to measure/calculate the size of a light beam at the sample of a microscope?

I am using a microscope with an LED derived light through the epi-fluorescent port of a microscope. I know that the "field of view" for a given objective is equal to the field number/magnification ...