The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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How can I keep pond water “alive”?

I've been preparing wet slides for my kids to look at from pond water. I have a mason jar of pond water. What can I do to keep the microbes in the jar alive going forward so that we can look at it any ...
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78 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
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background extraction in microscope analysis

I have taken microscope pictures for proteins in HeLa cells, in order to quantify the intensity (immunofluorescence). The proteins are both at the nuclear envelope, and in order to quantify the ...
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88 views

How are new staining protocols designed for microscopy?

How are new staining protocols designed for microscopy? Is it manual work or does it rely on computer simulations? Say for example I thought the H&E staining does not bring enough contrast. How ...
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358 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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Is Zeiss Primo Start microscope worth the price for student use?

I study physics but I have got interested about biology too. Surfing around the web, I have found out that in buying microscopes one needs to decide between the cheaper no-name brands vs. the four big ...
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Slicing cell cultures frozen in vitrified ice with a laser for CryoEM

Could a cell culture frozen in vitrified ice for CryoEM be effectively sliced into thin planes and resolved images produced? I was thinking if this could be done to get images from inside a cell.
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Are there any drawbacks to having a fixed-Köhler microscope?

I am considering to buy the Zeiss Primo star microscope. There are two variants: one with so called "Full Köhler" illumination and one with "Fixed-Köhler" illumination. Are there any drawbacks to ...
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For observing the cell which objective lens (10x or 40x) should be used with eyepiece lens 10x. And why?

For observing the onion cell which objective lens (10x or 40x) should be used with eyepiece lens 10x, in order to observe the onion cell. And why? Thanks for your help.
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Oral Fordyce spots

This is an image of a Fordyce spot I found while Googling. I don't know how to interpret this image. Is this an image of a single Fordyce spot? I mean "single" as in a single spot when viewed by the ...
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Effects of Acids and Bases on protozoa

Why is it that when you pour acid (HCl) on a culture of Euglena gracilis in an aqueous environment the protozoa begins to swell and when you pour a base (NaOH), it maintains its shape? I feel like ...
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Counting of surface bacteria in real time

I'm fairly new to microbiology (okay, I'm forced to look into it for a group project). What I'm looking for is a way to quantitatively get an estimate of the number of microbes (bacteria and/or ...
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Effects of Fixation on Cell Membrane

Why does fixing of cells with something like formaldehyde disrupt the membrane enough for probes, antibodies, and dyes to get into the cell?
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How to increase contrast with only a top entry specimen holder

My question is: If my microscope had a top entry specimen holder, instead of a side entry holder, what additional option(s) should I have to increase the contrast with the same constraint that I only ...