The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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What is a good source that will help me classify protists?

I am a 15 year old that enjoys looking at the micro world. The only issue that I have is that I can't find a good online source that will help me classify the name of the micro organism that I'm ...
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66 views

Deducing synaptic strength from electron micrographs?

There are several promising techniques for connectomics based on iterative sectioning and imaging of tissue with scanning electron microscopes (e.g. FIB-SEM and ATUM) By looking at such micrographs, ...
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Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
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How can I keep pond water “alive”?

I've been preparing wet slides for my kids to look at from pond water. I have a mason jar of pond water. What can I do to keep the microbes in the jar alive going forward so that we can look at it any ...
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22 views

background extraction in microscope analysis

I have taken microscope pictures for proteins in HeLa cells, in order to quantify the intensity (immunofluorescence). The proteins are both at the nuclear envelope, and in order to quantify the ...
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85 views

How are new staining protocols designed for microscopy?

How are new staining protocols designed for microscopy? Is it manual work or does it rely on computer simulations? Say for example I thought the H&E staining does not bring enough contrast. How ...
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286 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
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84 views

Is Zeiss Primo Start microscope worth the price for student use?

I study physics but I have got interested about biology too. Surfing around the web, I have found out that in buying microscopes one needs to decide between the cheaper no-name brands vs. the four big ...
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Slicing cell cultures frozen in vitrified ice with a laser for CryoEM

Could a cell culture frozen in vitrified ice for CryoEM be effectively sliced into thin planes and resolved images produced? I was thinking if this could be done to get images from inside a cell.
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Are there any drawbacks to having a fixed-Köhler microscope?

I am considering to buy the Zeiss Primo star microscope. There are two variants: one with so called "Full Köhler" illumination and one with "Fixed-Köhler" illumination. Are there any drawbacks to ...
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choice of negative stains in general

I have isolated a large molecular complex whose integrity is very sensitive to increasing ionic strength and thus had to be prepared for EM using low ionic strength solutions. My goal is image this by ...
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Reasons preirradiation of negatively stained and plastic embedded specimens with a low electron dose improve stability to electron irradiation

It is known that preirradiation of negatively stained and plastic embedded specimens with a low electron dose improves their stability to electron irradiation. But my question is why this occurs? ...
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How to increase contrast with only a top entry specimen holder

My question is: If my microscope had a top entry specimen holder, instead of a side entry holder, what additional option(s) should I have to increase the contrast with the same constraint that I only ...