The technique of using microscopes to view objects that are otherwise too small to be easily seen by the naked eye.

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17
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4answers
462 views

Machine learning for light microscopy — problems to solve?

I would like solve some biological problems that would improve the state-of-the-art of biology or bioinformatics. In particular, I want to apply machine learning on light microscopic images. The ...
13
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1answer
722 views

What is the magnification necessary to see pond life

My daughter is 5 and wants to learn about Biology. I thought a great start is to see life from pond water through a USB microscope. It would be great if she could see organisms like amoebas, ...
12
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4answers
436 views

Online repositories of scanning electron microscope photographs?

I have looked at every single Wikimedia commons photo in the category 'scanning electron microscope'. Unfortunately, I could not find any other repositories of such photos, but I would readily look at ...
6
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4answers
333 views

What kind of microscope for ML/biological research?

I am a computer science student, focusing on machine learning applications. I have been always interested in biology but I lack any training in it. Now, I had an idea that I could introduce myself to ...
6
votes
2answers
99 views

What is the state of automated quantitative analysis?

Quantitative analysis of microscope images is essential for many types of studies, but the techniques used most often are very labor-intensive. I have been able to find some literature about using ...
6
votes
3answers
111 views

What is a good source that will help me classify protists?

I am a 15 year old that enjoys looking at the micro world. The only issue that I have is that I can't find a good online source that will help me classify the name of the micro organism that I'm ...
5
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2answers
2k views

Microscope recommendation for an amateur?

I am a mathematics student looking for a hobby and this has come up as a potential hobby. Thus I am interested in buying a high-magnification microscope that is within the limits of my budget. My ...
5
votes
1answer
121 views

Mites on ginger root

I was just looking at some mouldy ginger found in the fridge under the microscope and was horrified/delighted to find (what looked to be) mites growing in it. I have searched the web and can only find ...
5
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1answer
64 views

What Websites Have Image Libraries for Bacteria and other Microorganisms

What Websites Have Image Libraries for Bacteria and other Microorganisms? With age of cell phone microscopes and hand held spectrometers it would be interesting and valuable to be able to compare in-...
5
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2answers
231 views

What to look for when buying a light microscope?

I am a science lover and want to buy a microscope to explore things around me. Like studying cell structures, microorganisms, blood and plants. It would be great if there is way to photograph slides. ...
4
votes
2answers
1k views

What kind of microscope should be used to view biological structures (such as spores) roughly 5 µm in length?

I would like to be able to identify genera of fungi based on the shape, size, and colour of spores. So the requirements are: Must be able to view a 5 micrometre object clearly with reasonable ...
4
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2answers
56 views

Dyes to bind to double stranded DNA?

Are there any commercially available fluorescent dyes that will bind only to double stranded DNA (not RNA, single stranded DNA etc.) for studying in vitro using confocal microscopy?
4
votes
1answer
79 views

How can I reduce the background gradient in phase contrast microscopy?

I'm looking for ways to set up a phase contrast microscope to reduce the brightness gradient that appears across the background of my images. I use phase contrast to find the outlines of cells in my ...
4
votes
1answer
132 views

How to operate a microtome?

In the picture above (Source: Amazon.com, United Scientific MCT001 Hand Microtome) we can see a microtome. How is it operated?
4
votes
1answer
387 views

Software for counting fly eggs

Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid ...
4
votes
1answer
95 views

What microscope/magnification would I need to observe P. Acnes bacteria?

I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
4
votes
2answers
303 views

How to visualize the ECM?

Specifically, I'd like to look at changes in HA (hyaluronic acid) production. Most often you only see people staining the cell surface or removing cells from culture for fixation and then imaging. ...
4
votes
1answer
6k views

Isotropy of sarcomere bands in skeletal muscle cells

As seen under a polarization microscope, the A-Band in skeletal muscle fibres is so named because it is anisotropic in its refractive index which is a characteristic of orderly crystalline structure. ...
4
votes
1answer
393 views

What is a simple protocol for staining cells in suspension?

I am an engineering student studying how electric fields affect cells, specifically the phenomena of electroporation in living cells. I know that electroporation is widely used for introducing genes ...
3
votes
2answers
2k views

What magnification do I need to see blood cells?

If I want to buy a microscope for my kids to be able to view single celled creatures and blood cells, about what magnification is required? A Celestron Pentaview digital scope claims up to 600×. Is ...
3
votes
1answer
128 views

Atomic force microscopy: depth penetration?

What depth penetration can one typically get with atomic force microscopes? (i.e. how far into the tissue can one image?). I have found numbers on acquisition times (minutes) and spatial resolutions (...
3
votes
3answers
290 views

Super-resolution imaging in vivo

Are there any microscopy modalities or techniques for in vivo imaging at higher resolutions than the diffraction limit? I was looking at this list: Super resolution microscopy, but they don't talk ...
3
votes
1answer
748 views

Is it true that viruses cannot be seen with phase-contrast microscopy?

There appears to be a lot of material on the internet claiming that viruses can be only seen with electron microscopes, and not with light microscopes. To the contrary, for example this old paper ...
3
votes
1answer
353 views

Benefits of CLARITY?

What are the benefits of CLARITY over this technique that was published more than a year earlier? Of course the second technique needs a fancier microscope that is likely more expensive and requires ...
3
votes
1answer
164 views

How to evaluate the efficacy of an antibody for fluorescence microscopy?

I'm trying to evaluate data taken from fluorescence microscopy with antibody staining, and am wondering whether there is any standard way to evaluate the specificity of the antibody for such ...
3
votes
1answer
93 views

Needle-like crystals observed in wild-grape wine under microscope. Potentially harmful?

I recently crushed and strained about thirty pounds of wild grapes to ferment into wine. About a month in, I did a visual inspection of the wine under microscope to try and identify some yeast and ...
3
votes
1answer
279 views

Can oil be used instead of water in preparing wet mounts for microscopy?

Is there a reason that oil (e.g., vegetable or clear mineral) is not used to make wet mounts in microscopy? I am not referring to oil immersion technique but substituting oil for water in wet mounts ...
3
votes
1answer
230 views

What are these stars in my microscopic image?

I see this very curious patterns on the sun-facing side of a jasmine (probably) leaf - visible with a makeshift microscope (about 50x) What are them? Pores? Are there other plants with similar ...
3
votes
1answer
89 views

Deducing synaptic strength from electron micrographs?

There are several promising techniques for connectomics based on iterative sectioning and imaging of tissue with scanning electron microscopes (e.g. FIB-SEM and ATUM) By looking at such micrographs, ...
3
votes
1answer
57 views

Lateral, axional and temporal resolutions for imaging in vivo

What are the spatial (lateral and axional), and temporal resolutions that one is expected to get with the following optical imaging techniques? Optical Bioluminiscence Imaging (BLI) Optical ...
3
votes
0answers
105 views

How can I keep pond water “alive”?

I've been preparing wet slides for my kids to look at from pond water. I have a mason jar of pond water. What can I do to keep the microbes in the jar alive going forward so that we can look at it any ...
3
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0answers
79 views

Microscopy Book Suggestions

I've learned programming through great book recommendations, many from the Stack Exchange series of sites. I'm hoping to take this approach to gaining a fundamental understanding of how fluorescence ...
3
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0answers
504 views

Why does vision go blurry after microscope use, and how to prevent it? [closed]

After using a binocular microscope recently for about 20 minutes, my vision went blurry and for an hour or so it was much harder to read small text. This occurs to a (far) lesser extent whenever I use ...
2
votes
5answers
61 views

Woodland outdoor microscopy advice

Let me start by saying I have never used a microscope outdoors, and in general I have never owned a microscope nor used one regularly. However, I am currently developing and organising some ...
2
votes
1answer
92 views

What is this image?

What I understand is- I think this is picture of DNA. But why is it in this conformation ? What types of cells have this type of DNA ?
2
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1answer
32 views

Can inverted microscope also be phase contrast microscope?

I've read somewhere that inverted microscope also allows phase contrast microscopy. Is that true? Do all inverted microscope have this ability? Do they have any other functions? Many thanks in ...
2
votes
2answers
50 views

How much are phase-contrast and dark-field microscopy used in biological research? [closed]

How much are phase-contrast and dark-field microscopy used in biological research today? It seems these were invented a long time ago, so I am thinking would it be a good idea to learn these ...
2
votes
1answer
35 views

Binocular microscope use, diaphragm?

I am try to review my scope work, since my proffesor asked me to calibrate all the scopes for an intro class in biology, and it has been awhile since I used one. I googled "Binocular microscope use" ...
2
votes
1answer
487 views

Does methanol fixation deform cultured cells?

I use methanol fixation (@ -20⁰C for 10 minutes) when performing immunofluorescence assays on cultured cells. Generally speaking, this results in very good antibody staining. However, the cells tend ...
2
votes
1answer
63 views

What are the features on a microscope one needs in order to do lab work?

By lab work I mean urinalysis, blood work(live as well), fecals, cytologies, histologies and all other. I have read(partly) a book(from 2002) on lab diagnostics and the author did not mention ...
2
votes
1answer
46 views

What's the best way to measure/calculate the size of a light beam at the sample of a microscope?

I am using a microscope with an LED derived light through the epi-fluorescent port of a microscope. I know that the "field of view" for a given objective is equal to the field number/magnification ...
2
votes
2answers
137 views

Image analysis of GFP-tagged protein localization bursts

I'm reading an article (full text here) that analyze the dynamics of localization of a GFP-tagged transcription factor (Crz1) over the time at the single-cell level, by taking movies in a fluorescent ...
2
votes
1answer
279 views

Any difference between inverted microscope objective vs normal microscope objective?

I'm planning to build an inverted microscope and I am not sure if I can use a plane achromatic objective of a normal microscope for the inverted microscope as well. Are there any specific needs for ...
2
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0answers
27 views

background extraction in microscope analysis

I have taken microscope pictures for proteins in HeLa cells, in order to quantify the intensity (immunofluorescence). The proteins are both at the nuclear envelope, and in order to quantify the ...
2
votes
0answers
89 views

How are new staining protocols designed for microscopy?

How are new staining protocols designed for microscopy? Is it manual work or does it rely on computer simulations? Say for example I thought the H&E staining does not bring enough contrast. How ...
2
votes
0answers
370 views

Image Processing Suite for bacterial microscopy: Schnitzcells or MicrobeTracker?

I am looking to start doing some work tracking the size and growth of individual bacterial cells in the microscope. In order to analyze the images I need software that can segment the cells, ...
1
vote
3answers
50 views

Why do people remove excess solution after they put on a coverslip?

Today, my classmate and I stained a slide of cheek cells. After we put on a coverslip, our teacher said that we are supposed to remove excess solution using a tissue. Why should we do this? I am so ...
1
vote
1answer
67 views

Why are bacterial cultures necessary?

I notice that in a lot of bacteria testing procedures the sample has to be cultured. You place the sample in agar or another growth medium and wait for the bacteria to proliferate before examining ...
1
vote
2answers
115 views

Calibrating Motic Camera to Stage Micrometer

Whilst calibrating my Motic Microsope's camera, I am able to calibrate the 10x object lens against the stage micrometer accurately. However when I calibrate (using the Motic 2.0 Plus software) the ...
1
vote
1answer
87 views

What are the dimensions in angstrom of bacteria? [closed]

I'm going to take a picture of a bacteria but I am unsure how large it is. What are the dimensions in angstrom of bacteria?