A method to purify small amounts of bacterial plasmids from the cells to get purified DNA at the end.

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1answer
77 views

Plasmid maintenance

I have obtained some plasmids used as integration vectors, this question may apply to all plasmids. I would like to have a somewhat continuos source for these plasmids, let's say that the origin of ...
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4answers
757 views

Why is it sometimes difficult to resuspend E. coli in P1?

It's a curiosity question. When I'm doing minipreps after pelleting the bacteria sometimes it's very easy to resuspend them in P1 (Qiagen kit), but sometimes they form a rubbery clump that is very ...
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2answers
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What is special about the N3 buffer for Qiagen minipreps?

For those who use Qiagen miniprep columns, they may be aware that the P3 buffer is the acetic acid step to neutralize cell lysis. They may also be aware that the Qiagen P3 buffer cannot be ...
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2answers
228 views

What are key factors when evaluating and comparing miniprep columns?

I'm looking to comparing different protocols for minipreps for plasmid DNA purification. What factors should I be looking at? A few things come to mind: Cost Yield Time per step Replacement with ...