The organelle in eukaryotes where the main stages of respiration occur.

learn more… | top users | synonyms

1
vote
0answers
7 views

mtDNA sequencing error rates

What is the method to estimate the error rate in mtDNA sequencing? E.g. we are given a profile like this: 16069T 16126C 73G 152C Is there a method, a table of known values, or any other way to say ...
3
votes
0answers
26 views

Validity of measurements of respiration in isolated mitochondria

I've recently read a couple of papers on exercise and mitochondria, in which state 4 and state 3 respiration rates and ROS production are assessed in vitro after exercise has been performed (i.e., rat ...
0
votes
1answer
67 views

Electron Transport Chain in Mitochondria [closed]

I was researching cellular respiration, and this is a rather confusing part. I need help understanding the purpose of Complex II and how the ATP Synthase generates the energy to turn ADP to ATP.
4
votes
2answers
104 views

Why don't protons diffuse out of the mitochondria during chemiosmosis?

If the outer membrane of the mitochondria is very permeable, how do mitochondria maintain a proton gradient by pumping protons into the intermembrane space? Wouldn't they just diffuse into the cytosol?...
1
vote
1answer
26 views

What is proton leak?

I have some idea about it. Is it the wasteful back flow of H+ ions through uncoupling proteins during aerobic respiration? Thank you
1
vote
1answer
38 views

What are respirasomes?

I have read wikipedia but don't understand it well. Do we mean Complex I, II,III and IV when we say respirasomes?
0
votes
1answer
23 views

Thermal ratchet model

Two models have been proposed to explain how ATP hydrolysis by mitochondrial hsp70 drives protein import.In the thermal ratchet model (Figure 12-28A), the emerging chain slides back and forth in the ...
1
vote
1answer
60 views

Can mitochondria be regenerated by the cell?

In the cell, division of mitochondria takes place on a regular basis, but what if there are no mitochondria present in cell (suppose all of them died), then can the cell make new mitochondria on its ...
1
vote
0answers
21 views

Do you have a comprehensive protocol using xGen blocking oligo? [closed]

Specifically used with genomic DNA and to help prevent non-specific binding
4
votes
0answers
63 views

Origin of the double membrane of mitochondria and chloroplasts

Most websites and textbooks say that the double membrane of mitochondria and chloroplasts are a result of the endocytosis of ancient prokaryotes (the outer membrane is from the vesicle containing the ...
3
votes
1answer
146 views

How many protons are pumped out per pair of electrons from NADH in oxidative phosphorylation?

I have searched the web and found that 10 protons are suppose to get pumped out during the electron cycle, but i'm a bit confused. I'm trying to count, for every complex(1/3/4), the number of protons ...
0
votes
0answers
20 views

Mitochodrial osmotic flux

If you incubate mitochondria in isosmotic solution of Tris/EGTA and KCH3COO solution with rotenone, no swelling occurs at first - i.e. no ions enter the matrix so water doesn't follow to compensate ...
0
votes
0answers
15 views

Heteroplasmy in different tissues

I have only started reading the substantial body of research on heteroplasmy. I was curious if anyone knew of attempts to measure the somatic cell mtDNA mutation rate, to see if it differed from the ...
1
vote
1answer
352 views

Are mitochondria alive? [duplicate]

I'm working on an assignment for my IB biology class and some assistance would be highly appreciated. I've read several articles and I still haven't quite gotten the answer I'm looking for. I have to ...
0
votes
0answers
15 views

Rates of evolution of mitochondrial genes

I am interested in knowing the rates of evolution of different mitochondrial genes (in base pairs per million years) across different taxa. Has there been any publications along these lines ?
0
votes
2answers
31 views

how to find full mitochondrial genome of plants in GenBank?

I am searching for full mitochondrial genome of a species of Malvaceae family. To be more specific, I need the genus Corchorus. ...
1
vote
1answer
62 views

Is studying complete mitochondrial genome need biological lab work?

The research complete mitochondrial genome study done on species: Juema pig Sus scrofa (Suina: Suidae) from southern Gansu red rainbowfish speckled dace, Rhinichthys osculus white char Salvelinus ...
0
votes
1answer
48 views

What tools can help me determine if a plant homologous protein interacts with an ncRNA in the same way?

First post here, so bare with me if I violate any etiquette or formatting rules. Let's say I've got a protein in humans. When it interacts with an ncRNA found in humans, it does something that I'm ...
19
votes
4answers
5k views

Are mitochondria dead?

In the video "What is Life? Is Death Real?", the subject of mitochondria is raised at 2:58. At 3:12, the narrator says "[mitochondria] are not alive any more: they are dead." What currents of thought ...
1
vote
1answer
76 views

Genetic Diversity in Mitochondria

As I was studying for my GRE exam, I was reading on Mitochondria. I then remembered that they undergo replication similar to bacteria. So I googled for info and found on Wikipedia that there is a " ...
8
votes
2answers
240 views

Are mitochondria transferable between cell types, individuals and species?

I'm curious about what happens when you insert organelles from a cell into another. In particular, mitochondria. Take two cells from your own body. Somehow extract a mitochondria from one cell and ...
0
votes
1answer
35 views

Is 100% accuracy in DNA sequencing possible?

I would like to obtain a complete genome of a canine (with mitochondrial DNA too) at 100% accuracy. Is it even possible with the current technology? The source I have is potential degraded, i.e. dried ...
5
votes
1answer
47 views

How are the physiological properties of mitochondria measured?

this is my first question on BiologySE. I am a Physics and Mathematics student currently doing a project on cell growth simulation. I am doing literature survey and I have a question about cellular ...
5
votes
1answer
66 views

Are there eukaryotes with fully working mitochondria but without mitochondrial genes?

Are there eukaryotic organisms with fully working mitochondria that do not have genes in the mitochondria? By fully working, I mean ones which can carry out oxidative phosphorylation, unlike reduced ...
4
votes
1answer
61 views

How does loss of the electrochemical gradient result in heat production?

As far as I understand, uncoupling of the flow of protons and ATP-synthase provides a bypass for protons between the outer and the inner membrane of mitochondria so that the protons don't have to go ...
4
votes
1answer
85 views

Why doesn't mitochondrial DNA accumulate damage over successive generations?

From what I recall, mitochondrial DNA is very susceptible to damage from oxidative stress because it is a site where free radicals are generated and their DNA isn't packed into chromatin like nucleic ...
3
votes
1answer
42 views

Forensic genetics- why is mtDNA comparison sometimes better than nDNA comparison?

Why is (in forensic genetics) in some cases more appropriate comparison of nuclear DNA but in some other cases comparison of mitochondrial DNA? Is it because geneticists are sometimes unable to find ...
2
votes
1answer
136 views

Why is oxaloacetate made in the mitochondria?

I'm looking at the gluconeogenic pathway: Pyruvate is shuttled into the mitochondria from the cytosol, converted to oxaloacetate by pyruvate carboxylase, converted to malate which is then shuttled ...
7
votes
2answers
740 views

Father with mutated mtDNA- why isn't his offspring at risk?

Mothers transmit their mitochondria (and therefore mtDNA) to their offspring and fathers don't. Lets assume that father had a mutation of the gene that encodes mtDNA, would then be his offspring at ...
3
votes
1answer
905 views

Mitochondria - are they really separate organisms that once merged into eukaryotic cells?

Theoretically, mitochondria are said to be a separate organism that is concerned with its own life and its own processes. In fact, it even duplicates individually. I know a similar question is here ...
3
votes
1answer
208 views

Do macrophages engulf mtDNA?

Let us assume that in a human body, a mitochondrial DNA is released to extracellular environment. We know that mtDNA inheritance is solely matrilineal. So the question is, If your mitochondrial DNA ...
3
votes
1answer
169 views

Why is brown fat brown?

I read in Tortora and Derrickson Anatomy and Physiology that : Another type, called brown adipose tissue obtains its darker color from a very rich blood supply , along with numerous pigmented ...
12
votes
3answers
7k views

Was the mitochondrion or chloroplast first?

I still don't know if the mitochondrion or chloroplast was first? I've looked for it on the internet and in various books but haven't found anything. Does anyone have the answer and a theory which ...
2
votes
1answer
383 views

Plastid and mitochondria

I am not a biologist, so please bear with me for this basic question. Although I tried googling, I am confused. What is the difference between plastids, chloroplasts and mitochondria? Are there any ...
6
votes
1answer
3k views

Which of the cell types commonly found in mammals has the greatest number of mitochondria?

This is basically a fun question, inspired by this answer on scifi.se. So, which cell type will have the greatest number of mitochondria? Obviously, I am talking about wild type, healthy individuals ...
13
votes
1answer
890 views

The Origin of Mitochondria

For a long time I've just accepted, because it is just what everyone told me, that mitochondria became organelles in the cell when they were "engulfed" by another cell which acted like it's host. This ...
6
votes
2answers
145 views

How much DNA of mitochondrial origin is incorporated in main cell's DNA?

And especially three points : in which chromosomes is it located (especially for the human case) ? how do we know about it ? does the proportion and composition vary a lot from one eukaryot to ...
3
votes
1answer
76 views

Common Ancestry of ETCs and ATP Synthase complexes

Rickettsiales are widely regarded as the ancestor of eukaryotic mitochondria through endosymbiosis. Cyanobacteria are widely regarded as the ancestor of eukaryotic chloroplasts through endosymbiosis. ...
6
votes
1answer
4k views

Why is FADH2 made instead of NADH in one of the reaction of Krebs cycle?

FADH2 is made in conversion of Succinate to Fumarate. Why is this so ? Why not NADH ?
2
votes
1answer
3k views

Why in some cells is GTP made in citric acid cycle and in some ATP?

In many animals GTP is produced in citric acid cycle. While in plants,bacteria and some animals ATP is produced. Why is this so ? What is the advantage ?
3
votes
1answer
1k views

Why do brain cells use shuttles that pass electrons from NADH to FAD?

Brain cells are cells require one of the highest amount of energy of any cell of body. So why do they use a shuttle which will transfer electrons from NADH produced in glycolysis to FAD(and there by ...
2
votes
1answer
1k views

Is there any advantage of having a mitochondria for aerobic respiration?

If we consider the pathway of breakdown of glucose which includes glycolysis, the citric acid cycle and the electron transport chain, all these processes takes place in some prokaryotes and ...
4
votes
1answer
153 views

Why is there sperm mitochondrial suicide?

The mitochondria in the sperm are digested upon entry into the egg, making mDNA inheritance exclusively female. What is the advantage of this? Wouldn't some male mDNA be beneficial because of the ...
1
vote
1answer
87 views

Do mitochondrial DNA have repair enzymes?

Mitochondria are considered endosymbionts originated from a prokaryote. So, do they have a DNA repair mechanism ?
6
votes
1answer
2k views

Why don't antibiotics affect mitochondria?

Given that mitochondria are evolutionarily closely related to bacteria, and antibiotics kill bacteria, why don't antibiotics also kill mitochondria?
8
votes
4answers
901 views

Mitochondrial DNA and recombination

Firstly I could do with a brief description of mitochondrial DNA. How does the structure of DNA in mitochondria compare to animal DNA (for the sake of simplicity let's say human - some animals might ...
7
votes
1answer
117 views

Cell Respiration and Oxidative Phosphorylation

Why and how does the proton travelling through the stalked particle in the inner mitochondrial membrane, cause a conformational change in the ATPase?
7
votes
1answer
431 views

What preceded ATP synthase?

ATP Synthase is ubiquitous throughout life on earth and so most probably evolved within the last universal common ancestor (LUCA) before that lineage diversified into the various kingdoms of life. ...
2
votes
1answer
53 views

PGC-1β Sod2 limiters/blockers

I'd like to block a percentage of PGC-1β or Sod2 expression. According to the following paper's figure beta-blockers inhibit some expression of PGC-1α. Are there any medicines/chemicals which safely ...
3
votes
1answer
49 views

mutant down but not out

I am interested in a gene which is null lethal but I need to temporary induce diminished capacity. If a cell is homozygous is it possible to induce heterozygous phenotypes or a partial knockout from ...