Tagged Questions
2
votes
0answers
37 views
What is the purpose of Y-shaped adapters in Illumina sequencing?
Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library.
The arms of the Y are unique, and the middle part, connected to the DNA fragment, is complementary.
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2
votes
1answer
53 views
Does bleach destroy RNAse activity, and if so, how does it do it?
I am working with RNA samples, and I'm trying to be very careful about RNAse contamination.
I have some questions about bleach, though. Some people say that a solution of bleach is enough to destroy ...
1
vote
1answer
21 views
Amount of reverse transcriptase in µg or mM for qRT-PCR
I am trying to calculate a titration amount for a molecule which I would like to use in my PCR samples. Different molecules have different densities so I would like to calculate the appropriate ...
3
votes
0answers
25 views
How does LCR compare to Assembly PCR
The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
2
votes
1answer
65 views
Can Taq polymerase be used instead of polymerase Vent exo (-)?
Instead of using polymerase Vent exo (-), can I go with the usual Taq polymerase?
Do the PCR conditions change (the temperature and master mix concentrations ) in the these two conditions? Do the ...
9
votes
2answers
120 views
What is the least costly method to generate sequential amino acid deletions?
I'm looking to generation sequential deletions from a gene of interest. The total size of this region is 8 amino acids. I'm trying to determine which portion of this region is necessary within the ...
6
votes
2answers
455 views
Reverse transcription PCR optimization
What is the ideal amount of RNA to use for the RT? and how much cDNA to use then for the PCR?
I did RT with a solution of RNA of 0.36 ug/ul. Then for my PCR I used 1 ul of the cDNA obtained and used ...
