The study of the molecular processes of the nucleus and cell function.
2
votes
1answer
78 views
In cancer, why do cells duplicate themselves?
In regards to cancer why do cells replicate themselves? If it's a mutation, what kind of mutation would this be classified as?
2
votes
3answers
66 views
Knockdown of long noncoding RNA (lncRNA) - how is it done?
I don't work at the wet lab and don't know all the details about the knockdown techniques.
My question is:
How lncRNA knockdown is done?
For example - you have lncRNA that is functional in the ...
2
votes
1answer
39 views
Does bleach destroy RNAse activity, and if so, how does it do it?
I am working with RNA samples, and I'm trying to be very careful about RNAse contamination.
I have some questions about bleach, though. Some people say that a solution of bleach is enough to destroy ...
2
votes
1answer
56 views
Can Taq polymerase be used instead of polymerase Vent exo (-)?
Instead of using polymerase Vent exo (-), can I go with the usual Taq polymerase?
Do the PCR conditions change (the temperature and master mix concentrations ) in the these two conditions? Do the ...
2
votes
0answers
22 views
What is the purpose of Y-shaped adapters in Illumina sequencing?
Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library.
The arms of the Y are unique, and the middle part, connected to the DNA fragment, is complementary.
...
2
votes
0answers
23 views
How does LCR compare to Assembly PCR
The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
2
votes
1answer
48 views
Histone marks mechanism
I am slightly confused about the mechanisms that makes histone modifications associate with gene expression.
That is, H3K36me3 is believed to be present in actively transcribed genes, H3K27me3 in ...
2
votes
0answers
58 views
Optimization of annealing temperature
I am trying to optimize annealing temperature for some primer pairs. I have tried optimization using cDNA, genomic DNA, Taq polymerase, phusion polymerase etc., but every time I am either getting ...
2
votes
0answers
73 views
Mouse meta-globin mRNA problem
This is an mRNA strand of mouse meta-globin:
5'-ccccagauacggaauucgaau-3'
A) Which small RNA (below) is most likely to regulate expression of
meta-globin?
...
1
vote
1answer
21 views
Amount of reverse transcriptase in µg or mM for qRT-PCR
I am trying to calculate a titration amount for a molecule which I would like to use in my PCR samples. Different molecules have different densities so I would like to calculate the appropriate ...
1
vote
1answer
51 views
Why is Sanger sequencing inferior for detecting SNPs in cancer cells?
I am familiar with Sanger sequencing, but at the level of an undergraduate. A lecturer of mine tried to describe Sanger sequencing as losing the sequence information in noise when used to detect ...
1
vote
1answer
25 views
What is solid-phase bridge amplification?
During Illumina sequencing there is a step called bridge amplification by which DNA is amplified by isothermal enzymes. What is this stage, and how does it work?
1
vote
1answer
31 views
Why does the hydrolysis of ATP increasing entropy increase the Phosphoryl-‐transfer Potential?
I am currently taking a biology class. I do not understand this concept. I understand that the electrostatic repulsion of the negative charges, resonance stabilization and hydration stabilization all ...
1
vote
0answers
21 views
What mammalian cell lines are easiest to grow and transfect?
I'm working with HEK293 cells, which are very easy to transfect. Does anyone have other suggestions for cell lines with very high transfection efficiency? Ideally the chromosomes would also be diploid ...
1
vote
1answer
25 views
How can I save bacillus strains on filter paper without an -80 degree freezer?
I want to save my bacillus strains but I don't have access to a -80 degree freezer. What are possible alternatives?
1
vote
1answer
51 views
Manipulation of gene expression using VP16 fusion and engrailed fusion to a transcription factor?
Today, a presenter briefly mentioned that gene expression in sea urchins during development might be manipulated using VP16 and engrailed fusions.
On a slide, it said that expression might be ...
1
vote
0answers
32 views
Can I heat Trizol?
I wonder if I can heat Trizol reagent for 30 min 65C. The goal is to disrupt protein-RNA complex while inhibiting nucleases. (I can't use RNasin cause it's inactivated in 65C, and can't use RVC cause ...
1
vote
0answers
25 views
Experience with Detox Cloning
I just saw this paper.
A Highly Efficient Molecular Cloning Platform that Utilises a Small Bacterial Toxin Gene
I was curious if anyone had experience with this cloning strategy using the IbsC ...
1
vote
0answers
34 views
Molecular cause of cramps, spasms and strengthening in muscles? (incl. intro to muscle contraction)
When motor neurons are stimulated to trigger an action potential, this potential propagates down the spine, eventually reaching a neuromuscular junction, causing the release of acetylcholine (ACh).
...
1
vote
0answers
42 views
Solid phase use in HIV rapid tests
I have another question in regards to my HIV test research. The rapid tests like Orasures Oraquick contains a strip of synthetic peptides that are used to represent proteins found in the envelope ...
0
votes
1answer
64 views
If inhibiting S6 kinase decreases protein translation, then could inhibiting S6 kinase could possibly slow down long-term potentiation in neurons?
From http://en.wikipedia.org/wiki/P70S6_kinase...
Phosphorylation of S6 induces protein synthesis at the ribosome.
P70S6 kinase is in a signaling pathway that includes mTOR (the
mammalian ...
0
votes
1answer
25 views
How can I clean phenol extracted RNA?
I extracted RNA from skin and quantified it in an Nanodrop machine. The 260/230 ratio was very low and two peaks appeared in the absorbance curve, but the RNA is ressuspended in RNAse free water. How ...
0
votes
0answers
16 views
In vitro enzyme production
I need to express a protein in vitro but I don't know where to start. I will likely do a T7 transcription protocol but for translation I am not sure what to do. Are there any good kits?
0
votes
0answers
11 views
Hardy-Weinberg applied to three alleles and stimation of allele frequencies
I have this equation:
Corresponds to HW in equilibria with three alleles:
$(p+q+r)^2=1$
Expanding the square results:
$p^2+2pq+r^2+2pr+q^2+2qr = 1$
I need to separate homozygous and ...
0
votes
0answers
86 views
which free videos with 3d animations simulating protein processes are there? [closed]
I have found a group that creates free videos that show life within (or outside) a cell as it really is, at the near molecular level. They use real protein structures with in-molecule dynamics ...
0
votes
0answers
99 views
Text Book on Cancer Genomics/Biology [closed]
Could anyone of you suggest few of the best text book(s) for Cancer Genomics/Biology from basic to advance level.
-1
votes
2answers
41 views
What are some basic drosophila methods? [closed]
I'm a grad student rotating in a drosophila neurobiology lab. I'd like to do a little self-study to bring myself up to speed. What are some basic, essential drosophila methods and applications? And ...
