Tagged Questions
5
votes
1answer
61 views
Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?
I'm currently working on a synthetic biology project which involves working with lots of different parts. I would ultimately like to integrate these genes by transforming a single plasmid. I've heard ...
7
votes
1answer
90 views
How is the exogenous DNA protected from degradation during bacterial transformation?
During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
5
votes
1answer
557 views
What is the distinction between F' plasmid and R plasmid?
Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...
