Tagged Questions
2
votes
3answers
41 views
Knockdown of long noncoding RNA (lncRNA) - how is it done?
I don't work at the wet lab and don't know all the details about the knockdown techniques.
My question is:
How lncRNA knockdown is done?
For example - you have lncRNA that is functional in the ...
1
vote
1answer
21 views
What is solid-phase bridge amplification?
During Illumina sequencing there is a step called bridge amplification by which DNA is amplified by isothermal enzymes. What is this stage, and how does it work?
3
votes
1answer
43 views
What's the difference between shotgun sequencing and clone based sequencing?
What are the main differences between shotgun sequencing and clone based sequencing?
6
votes
1answer
56 views
How is the exogenous DNA protected from degradation during bacterial transformation?
During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
2
votes
1answer
37 views
methods for targetted deletion of genomic regions?
I would like to know what are currently used methods for targeted deletion of genomic regions in mammalian organisms or cell lines. I have heard of Zinc-Finger nucleases as a recent genetic ...
7
votes
0answers
57 views
paralogue genes in genome-wide association studies?
Has anybody tested if there is an over-representation of significant paralogous copies of genes -- either tandemly-arranged or in different chromosomes -- in the list of significantly associated genes ...
4
votes
1answer
473 views
Co-transformation of plasmids from the same incompatibility group
Can two plasmids with the same origin of replication (for example pBR322 ori) and thus from the same incompatibility group be successfully co-transformed in E. coli? What are the mechanisms that would ...
6
votes
1answer
68 views
Bicoid regulation of hunchback
I'm learning about development via the example of Drosophila embryogenesis. I understand that bicoid regulates hunchback, among other genes. My question whether the regulation is direct or indirect? ...
6
votes
4answers
171 views
What's the aim of genetically modifying of foods/organisms?
On news, articles etc. experts talking about Genetically Modified Foods and Organisms often mentions about their disadvantages like,
their potential to harm human health
allergies may become more ...
5
votes
1answer
541 views
What is the distinction between F' plasmid and R plasmid?
Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...
10
votes
1answer
157 views
What is the functional and structural distinction between core (H2A, H2B, H3,H4) and linker(H1/H5) histones?
Many explanations of histone biochemistry isn't quite elucidating for the undergraduate student. How does histone structure (dimers, octomers) relate to their specific functions as core or linker ...
