5
votes
1answer
100 views

Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?

I'm currently working on a synthetic biology project which involves working with lots of different parts. I would ultimately like to integrate these genes by transforming a single plasmid. I've heard ...
7
votes
1answer
266 views

How is the exogenous DNA protected from degradation during bacterial transformation?

During transformation, a bacterium can take up DNA from its environment. A small fraction of bacterial species are known to be naturally competent, meaning that they can engage in this sort of ...
4
votes
1answer
131 views

Vigorous shaking for HFR interrupted mating

I am trying to reproduce E. coli interrupted mating using an HFR strain, and I read that the cultures should be vigorously shaken at times to interrupt the DNA transfer from the F+(HFR) donors to the ...
6
votes
4answers
638 views

What's the aim of genetically modifying of foods/organisms?

On news, articles etc. experts talking about Genetically Modified Foods and Organisms often mentions about their disadvantages like, their potential to harm human health allergies may become more ...
5
votes
1answer
944 views

What is the distinction between F' plasmid and R plasmid?

Is there a difference between an F' plasmid that has taken up a chromosomal gene that conveys antibiotic resistance, and an R plasmid? Is a bacterium containing an R plasmid and yet lacking an F+ ...