The scientific study of the structure and function of genes at the molecular level, particularly chromosomes and DNA.

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How to identify the genes that distal enhancers pair?

I am writing a project proposal and I have to talk about this problem: how to identify the genes that distal enhancers pair? I am really new to this topic and I don't know what it is all about. I ...
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108 views

Heterochromatin binding sites position information

I am working on a project and I'm trying to find data on the position of genes and heterochromatin binding sites such as HP1 in Drosophila melanogaster. Is this information available for the DGRP ...
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47 views

What is the close and related genome used for in Gene models?

all I am a little bit confused about using the related genome or reference genome. When we have a reference genome, we can do alignment. Also we can do the assembly. Can you give some more reason ...
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39 views

Book Recommendation: Complex Traits and Complex Genetic Architecture

I am looking for a book (or any good source of information) that offers an in-depth discussion and models about the evolution and analysis of complex traits and complex genetic architecture. Do you ...
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43 views

Gene Complementation Question

Hi everyone! Here is a diagram of a genetics complementation problem. My main concern is regarding number 3d. I'm assuming that in this case, it would be complementary interaction and thus ...
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89 views

Pedigree Diagram help

I am asked to fill the genotypes in the spaces provided but looks like I am aving a little bit of trouble. can anyone help? thanks in advance!
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Regulation of the replication of mtDNA at embryonic level

While reading an article on mitochondrial inheritance I came across this link. The results state that mitochondrial DNA replication is regulated in different cells of an embryo at different levels. ...
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53 views

Criteria for the numbering of human chromosomes

What were the criteria devised for the numbering convention employed in human chromosomes? When was it fixed? Correct me if I am wrong; it appears that chromosome pairs 1 to 22 were originally ...
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231 views

what is knob heterochromatin?

I am reading a paper which discusses Maize Genome Structure. Descriptions of the structure is given in the papers introduction. I know about heterochromatin "heterochromatin stains intensely, ...
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384 views

What is solid-phase bridge amplification?

During Illumina sequencing there is a step called bridge amplification by which DNA is amplified by isothermal enzymes. What is this stage, and how does it work?
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Loss in DNA band size extracted from polyacrylamide gel

I'm using cDNA AFLP teq.. I extracted 670 bp band from polyacrylamide (urea) gel and I have amplified with PCR but on agarose gel it stops at nearly 350 bp.it happened for most of other bands before. ...
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16 views

How does Temperature influences the rate of protein turnover?

Question How (quantitatively speaking) does temperature influences rate of turnover of transcription factors? Which protein? As I am not looking for any accurate number I am talking about an ...
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Question about mutation of multiple nuclei?

If an organism with multiple nuclei, such as the slime mold Physarum polycephalum, was to be exposed to a mutagen, such as 5-bromouracil, what would be the effect (besides death)? Especially if ...
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Linkage disequilibrium D sign

Linkage disequilibrium D equals D=x11−p1⋅q1 where: Haplotype > Frequency A1B1 > x11 A1B2 > x12 A2B1 > x21 A2B2 > x22 and Allele Frequency A1 > ...
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67 views

what is the function of satellite in a chromosome?

What is the specific function of a satellite in a chromosome ? How are sat-chromosomes different from others ? On molecular basis, what are the functional advantages of it ?
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35 views

Assembling DNA fragments with ~10kb overlap in vitro

Let's say I have two fragments of bacterial DNA (~50kb in length each) that overlap on the ends by roughly ~10kb; the overlap sequences are unknown. I would like to assemble these into longer ...
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Study case of the inheritance system of Oenothera

I've been told some interesting facts about oenothera. Apparently in this species some lineages have been through some translocations and in results to these translocations and in consequence, some ...
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63 views

Studying genetics [closed]

I am interested in studying disorders that are genetic in origin. However, I am having difficulty in understanding molecular and cellular mechanisms regarding processes such as meiosis, mitosis, and ...
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34 views

Transduction with P1 lysate prepared from strain that is proC::Tn10

If I do a transduction with a P1 lysate prepared from a strain that is pro*C*::Tn10 and the recipient is wild type, will all my recombinants be ProC- and Tet resistant since the Tn10 is in the proC ...
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50 views

Polymerase Observations

DNA polymerase I (PolI) contains polymerase, 3'-5' exonuclease, and 5'-3' exonuclease activities in a single polypeptide chain of molecular weight 109 kDa. If E. coli Pol I is exposed to the protease ...
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55 views

Arabidopsis thaliana RCSB active site gene mutant

I'm looking for an Arabidopsis thaliana gene listed in RCSB with a clear mode of function and active site. In addition it needs to have an obvious phenotype when knocked out like severely retarded ...
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How do amino acids become attached to tRNA?

Is there a protein that catalyzes this or does it happen spontaneously? In which part of the cell does this happen?
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41 views

What are the structual differences between DNA and RNA? [closed]

How is the structure of DNA different from that of RNA?
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42 views

How can I download a gene sequence from GenBank (NCBI)? [closed]

Could you tell me the steps to find and download a gene sequence from GeneBank? I would appreciate your help.
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132 views

Can you detect if a mutation is spontaneous or induced?

Is it possible to determine if a certain specific mutation had a spontaneous origin (for example from a mistake of the DNA polymerase) as opposed to an induced origin (for example, from some genotoxic ...
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24 views

Why can't NMR be used on large RNA molecules?

While reading my Molecular Bio textbook, I came across a sentence that stated that NMR cannot be used on large RNA molecules to determine their structure. Why is this? Is it because RNA is single ...
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Property of E.coli harboring F element

From Genetics by Ursula Goodenough E.coli harboring an F element are endowed with a number of phenotypic traits : 1.They are sensitive to infection by ssRNA phages and certain ssDNA phages. ...
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96 views

PedigreeAnalysis Genetics [duplicate]

Hello! I need help with this exercise question. I am not really sure which pedigree diagram is autosomal and which is not. But, is question b 1/2. Can someone give an explanation? Thanks very much ...
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57 views

What is “transcribed strand”

This is an excerpt from a paper: T>C mutations at NpTpN trinucleotides, also found in hepatocellular carcinomas, shows strong transcriptional strand bias with more T>C mutations on the transcribed ...
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Incomplete dominance vs. codominance at the level of protein production and function? [on hold]

What is the difference between incomplete dominance and codominance at the level of protein production and function?
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Calculating number of amino acids in mRNA

Assuming there were 20 different amino acids and less than 40 types of different tRNAs found in this alien organism. How many amino acids would be found in the translational product of a ...
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27 views

Finding appropriate research papers?

I had previously asked some question on cystic fibrosis and I had mentioned that I am a senior in high school writing a paper on gene therapy and CF. I would like to some specific paper on viral ...
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14 views

Ways to measure effectiveness of a gene therapy trial?

I understand that Fluorescence in situ hybridisation can be used to measure the effectiveness of a gene therapy experiment/trial. But what are some other genetics techniques to measure the ...
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23 views

Too much light in electrophoretic gel photo

The electrophoretic gel's photo shows too much light. Is it because the sample is not loaded properly? I can't see any gel lanes in my PCR samples or in the marker itself when photographing them.
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22 views

Cell targets of Glybera

So we know that there is a first gene therapy drug in the market out there called Alipogene tiparvovec to address lipoprotein lipase deficiency (LPLD) at a genetic level. Does this genetic drug ...
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19 views

How loss of heterozygosity influence expression levels of a gene ?

I am looking at a genomic region associated with a vascular diseases; this region have loss of heterozygosity. I am wondering how the expression level of the genes in the regions will be affected ...
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70 views

New vs classical genome editing methods

I would like to inquire on the differences between the old classical genome editing method in comparison to the new genome editing methods such as CRISPR/Cas9. By old classical method i mean the use ...
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Inbreeding depression and dominance

From this article, second paragraph of the second page A classic theoretical result is that the mean of a character controlled by a single locus $i$ with two alleles $A_{i1}$ and $A_{i2}$ is only ...
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Why does the stability of YAC increase with size?

From Lehninger Principles of Biochemistry : The stability of YAC increases with size (up to a limit). Those with inserts of less than 100000 are gradually lost during mitosis. Why does this ...
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34 views

Cloning a gene of an organism with an nonsequenced genome

What would be the best protocol to clone a gene about 5kb in size? The genome is not sequenced, but the gene itself very similar to orthologous genes of organisms with known sequences.
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Paracentric Inversion and Mutations Question Help Needed

Would someone please be able to help with questons 3 or 4. (one of the at least please) please don't report his as off-topic. first, this is NOT a homeowork question. It's a practice question I am ...
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Southern/Western blot analysis and pedigree diagram? Help needed. [duplicate]

Hello everyone. I urgently need help with this question please. I have filled in the blanks using the results in the figure provided in the sheet. However, for number (b) is a little hard to ...
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Paper on non-viral vectors for CF gene therapy

I am now planning to write a paper now specficially on liposomes vectors for Cystic fibrosis. I am planning to use papers that talked about early trials in animals with liposomes and early clinical ...