Messenger RNA is produced during transcription before it leaves the nucleus to be be acted upon by a ribosome in the process of translation. This produces a sequence of acids for which mRNA can be considered the template
5
votes
2answers
52 views
How to find Exons in mRNA Computationally
I'm having trouble finding a method to find exons in the original DNA sequence used to create the mRNA, even given the sequence of the mRNA, as I cannot find a way to reliably identify the beginning ...
7
votes
1answer
61 views
Do antisense transcripts have different names than their sense strand transcripts?
I want to find which genes in the human genome can potentially be complementary to a transcript that could act as antisense transcript inhibtion? Are cis-NATs (naturally occuring anti-sense ...
17
votes
2answers
908 views
Do gene expression levels necessarily correspond to levels of protein activation?
I have seen a lot of research into molecular mechanisms of diseases/phenotypes use measures of RNA as a 'proxy' for the level of protein available in the cell. Is this actually valid?
My problem ...
2
votes
0answers
56 views
Has anybody used Evrogen's DSN-normalization protocol for cDNA normalization? [closed]
I found the link to a commercial product by Evrogen to normalise cDNA samples for gene discovery projects here:
http://www.evrogen.com/technologies/normalization.shtml
The most up-to-date reference ...
7
votes
1answer
82 views
How can I normalize mRNA samples for sequencing?
Is there an easy, inexpensive, not too labor intensive way to normalise mRNA samples so that even though one loses information of gene expression levels, each of the transcripts in the transcriptome ...
9
votes
1answer
79 views
Which patterns do I have to avoid when modifying the 3'-UTR?
I want to change a pre-miRNA sequence (in my case the pre-miRNA is encoding in a 3'UTR of a gene) and then put it in a lentivirus to see if it is still processed.
After modification (permutation of ...
15
votes
3answers
195 views
Is exon order always preserved in splicing?
Are there any cases in which the splicing machinery constructs an mRNA in which the exons are not in the 5' -> 3' genomic order? I'm interested any such cases, whether they involve constitutive or ...
