Messenger RNA is produced during transcription before it leaves the nucleus to be be acted upon by a ribosome in the process of translation. This produces a sequence of acids for which mRNA can be considered the template

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Which patterns do I have to avoid when modifying the 3'-UTR?

I want to change a pre-miRNA sequence (in my case the pre-miRNA is encoding in a 3'UTR of a gene) and then put it in a lentivirus to see if it is still processed. After modification (permutation of ...