Messenger RNA is produced during transcription before it leaves the nucleus to be be translated by a ribosome. This produces a sequence of nucleic acids for which mRNA is considered the template.

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What is the meaning of conservation site in miRNA-mRNA pairing context?

I am not biologist and just work in Bioinformatics field. Does anyone know what is the meaning of conservation in the context of miRNAs and their pairings to mRNAs? Example: Each of these duplexes ...
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31 views

Blood sampling at home

I want to collect small blood samples at regular intervals (e.g. every 8 hours or more often) over a longer period (weeks to months). To avoid having to go to a lab or doctor to have this done, I want ...
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Gene expression for mouse feeder cells (inactivated MEFs)

I'm looking for a paper with gene expression data for mouse feeder cells, inactivated by gamma radiation or mitomycin C. Ideally I'd like RNA-seq data but I'll use microarray data if that's all there ...
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25 views

mRNA extraction from mice ears.

I am trying to extract RNA from mice ears and for some reason I don't have RNA when I perform the electrophoresis. I directly cut the ears and I put it in a tube with a bead and trizol. then I place ...
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38 views

Which data format for mRNA expression matrix is necessary to run pathway analysis with Paradigm?

I am running Paradigm on a matrix of mRNA expression. I read Vaske, Charles J., et al. "Inference of patient-specific pathway activities from multi-dimensional cancer genomics data using ...
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15 views

How to automate this custom gene expression microarray chip?

I have 16 gene sequences that I am interested in their detection in clinical samples. I already measured their RNA levels by using RT-qPCR technique and their benefit has already been proven to help ...
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32 views

Extracting the donor/acceptor sites from mRNA sequence(PERL/ DNA transcription)?

I have the following program that I am analyzing and trying to understand. Overall purpose of it is to put out, based on the option selected, the set of introns, exons, acceptor sites , or donor sites ...
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25 views

Predicting alternative transcript sequence and probability from a single SNV

I am currently studying a small number of non coding SNPs in terms of their contribution to the cancer phenotype. All are obtained from massive parallel sequencing on a specific gene panel, utilizing ...
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Splicosomes, Transesterification

How is it that a spliceosome complex ensures that the 5' end of the cut intron will attack the branching point on the same intron but not the branching point of the next intron? And some introns are ...