I am trying to multi-fragment Gibson Assemblies and my PCR product concentrations are not high enough to yield plasmids every time. I am using a very low amount of elution buffer to maximise ...
The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
I was wondering if anyone has ever seen large deletions (~40-50 bp) occur when using phusion polymerase to clone a gene. I have recently tried using Circular Polymerase Extension cloning to clone a ...