Tagged Questions
4
votes
1answer
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How are DNA polymerase error rates measured?
It is well known that the first DNA polymerase, Taq, is quite error prone. Newer generation commercial enzymes that have either been isolated from different thermophile species or have been improved ...
7
votes
1answer
316 views
How to clone and sequence a gene transcript of unknown sequence?
How might I go about amplifying a gene transcript (mRNA) from animal tissue of which little is known about the genome? In some applications, I have used reverse transcriptase PCR to amplify all mRNA ...
