Tagged Questions
9
votes
1answer
143 views
Do single crossovers occur in circular polynucleotides?
Single crossovers in circular pieces of DNA do not seem to be a big topic, because if they happened, they would lead to a kind of combined chromosome with two inner strands and one large outer strand. ...
4
votes
1answer
91 views
Vigorous shaking for HFR interrupted mating
I am trying to reproduce E. coli interrupted mating using an HFR strain, and I read that the cultures should be vigorously shaken at times to interrupt the DNA transfer from the F+(HFR) donors to the ...
4
votes
1answer
491 views
Co-transformation of plasmids from the same incompatibility group
Can two plasmids with the same origin of replication (for example pBR322 ori) and thus from the same incompatibility group be successfully co-transformed in E. coli? What are the mechanisms that would ...
