Often circular segments of DNA that can replicate outside of the chromosomes. Generally found in bacteria.

learn more… | top users | synonyms

3
votes
0answers
317 views

E. coli not growing in liquid medium

We are regularly doing bacterial transformation and subculture from plate to liquid media to extract DNA. This usually goes very well and is straightforward, but occasionally, the colonies that grew ...
3
votes
0answers
31 views

Gibson assembly - primer design with A and T rich regions

I have question about Gibson assembly. I have done it several times and it always worked okay for us, but now I want to assemble together a fragment which has sequence like this: ...
2
votes
0answers
45 views

Why is there a size limit on inserts that a plasmid can accept?

Throughout my undergraduate education I have been taught that plasmids can't carry very large inserts, but I have never been told why. Any insight would be greatly appreciated.
1
vote
0answers
37 views

What is the sequence of pPROLar.A plasmid?

I've been looking for the sequence of pPROLar.A122 plasmid but I haven't found it. Where could I find the sequence? The first link (addgene) on googling reports a plasmid which is reported to have ...
1
vote
0answers
32 views

Plasmid with ITPG and pBR322 ori

I'm looking for suggestions as my internet searches aren't turning up much. I'm trying to find a plasmid that has the pBR322 origin, as it's low(ish) copy number and can tolerate large inserts. ...
0
votes
0answers
24 views

Complementing back the expression of a knockout gene

I have a human cell line (Huh7.5, hepatoma-derived) in which Ago2 gene has been knocked out by the Crispr/Cas9 technology. I checked the effective gene editing by sequencing and I also confirmed the ...