Enzyme that catalyzes serial addition of monomers to synthesize a polymeric biomolecule. This term usually applies to DNA/RNA polymerases which synthesize these nucleic acid polymers from mononucleotides.
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Polymerase Observations
DNA polymerase I (PolI) contains polymerase, 3'-5' exonuclease, and 5'-3' exonuclease activities in a single polypeptide chain of molecular weight 109 kDa. If E. coli Pol I is exposed to the protease ...
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Where does Taq polymerase migrate during electrophoresis?
Assuming a 1% Agarose gel with TAE.
Follow up questions:
Is there a way to stain for it?
Could the polymerase be captured and reused?
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Has anyone ever sucessfully translated xRNA or yRNA?
I've recently been researching the subject of size-expanded nucleobases in alternative genetic sets. Many papers describe the, at least, partial success in replicating xDNA and yDNA, as well as ...
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How are DNA polymerase error rates measured?
It is well known that the first DNA polymerase, Taq, is quite error prone. Newer generation commercial enzymes that have either been isolated from different thermophile species or have been improved ...
3
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1answer
161 views
Open protocol for Ligase Independent Cloning
Ligase Independent Cloning is a protocol that allow an insert to be integrated into a vector without ligation. It uses T4 DNA polymerase with only ATP to first chew back from blunt ends to create ...
6
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1answer
64 views
number of RNA pol II molecules in a given human nucleus?
Does anybody have any estimate on the number of RNA pol II molecules in the nucleus of a given human cell? For example, how many RNA polymerase type II protein complexes would there be on average at a ...
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Why is DNA replication performed in the 5' to 3' direction?
DNA replication goes in the 5' to 3' direction because DNA polymerase acts on the 3'-OH of the existing strand for adding free nucleotides. Is there any biochemical reason why all organisms evolved to ...
