7
votes
1answer
114 views

First RNA polymerase-mRNA

We know that RNA polymerase produces mRNA by reading DNA strand. Which enzyme produces the first RNA polymerase if other RNA polymerases are synthesized in the same way like other enzymes(proteins) ?
1
vote
0answers
40 views

How does one quantify T7 RNA polymerase transcription efficiency?

I am looking for a method which can help me calculate how many sequences one will get after transcription of 100-mer random sequences by T7 RNAP. The sequence pool has a known complexity and will be ...
2
votes
2answers
103 views

How is the transcription direction of RNA polymerase determined?

When transcription factors attach to the DNA strand - How do they know in which direction they have to initialize the transcription by rna polymerase? Is it always read in the same direction anyway? ...
1
vote
1answer
110 views

DNA Replication

I just wanted to understand the basic steps behind the replication of the lagging strand of DNA: Have helicase unwind it first DNA Primase lays down RNA primers in fragments, called Okazaki ...