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-3
votes
1answer
38 views

How far can we go in engineering our own evolution? [on hold]

After recently seeing Christopher Nolan's Interstellar and reading Kip Thorne's The Science of Interstellar, I've seriously been reflecting on the challenges mankind faces in becoming space pioneers, ...
1
vote
1answer
20 views

Could the proteome of E. coli be fluorescently labelled?

What proportion of the total number of proteins in E. coli could be fluorescently labelled for PALM/STORM imaging?
2
votes
1answer
24 views

Has any research lab done serious work to engineer new bacteria which assemble graphene wafers?

I was thinking about crazy uses for engineered bacteria. Graphene seems like a potentially excellent target for the technology. Have any credible labs worked on this? Any papers on it? What would be ...
7
votes
0answers
490 views

Rosetta ab initio prediction and protein-protein interaction fitness help [closed]

I have designed several proteins which I predict have interactions with another protein using the sequence based Conjoint Triad Method. I would like to know which ones structurally are predicted to ...
21
votes
1answer
328 views

Phagemid display

If I'm using a bacteriophage for phage display and I'm trying to avoid avidity effects by using a helper phage what would be the best way to maintain a large library size while keeping everything ...
7
votes
1answer
180 views

What is the fastest way to build an alanine scanning library?

For interfacial studies, I would like to build an alanine scanning library for one of my proteins examining 20 sites. I will ultimately express the gene using E.coli cell-free protein synthesis. I ...