Questions tagged [protein-expression]
The process by which proteins, encoded in the genome, are ultimately synthesized.
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Optimization of Signal Peptides
We are looking at modifying the signal peptide of a receptor in a common immortalized human cell line. The cell line already expresses an unusually high amount of the protein, but much of it is not ...
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Relative densitometry from SDS PAGE
I'd like to perform densitometry on a Coomassie stained SDS PAGE gel to compare a recombinant protein's expression levels under two conditions. I'm using BioRad's Image Lab software.
My questions are ...
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How are animal patterns encoded in the dna?
After seeing the patterns on the feathers of a argusianus argus pheasant (shown below), I am curious where is the information that encodes a pattern for a particular bird, and what form is this ...
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Does glycerol in E.coli culture media somehow inhibit the lac-operon?
I have have been taught that one should induce protein expression with IPTG at an OD of about 1.0 - 2.0 when E.coli grows it TB media (terrific broth). As a reference point, one typically induces ...
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Has network theory been successfully applied to protein expression within a cell?
I saw the following passage in the NPR 13.7 Cosmos & Culture commentary post The Big Idea Behind Big Data.
Using the Internet's first generation of Big Data, researchers like physicist Albert-...
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Do exon deletions in C.elegans mutants necessarily result in nonsense mediated decay of the transcript?
I am trying to write out my research proposal about a project in C.elegans. There are multiple mutants of the factor that I want to study, each with different mutations. I know that a mutation that ...
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Why HIS tag is not detected in WB or dot blot?
I would like to know why the recombinant protein with his tag at c terminus is not being detected neither in western blot or dot blot.
I'm trying to validate protein expression of four proteins. ...
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Which method would more accurately help to identify the unknown concentration of a protein sample between the A280 and the Bradford methods?
I quantified my protein using the standard Bradford method and the A280 methods and obtained values that were far off from the theoretical value of the protein of interest, and therefore was wondering ...
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Expressing bacterial proteins in mammalian cells
Does anyone have experience/knowledge of expressing bacterial (or other microbial) proteins in mammalian cells? I am interested in this for a project where we are trying to introduce a new enzymatic ...
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Protein expression in K. lactis system
I am trying to express a protein in K. lactis Protein Expression Kit (E1000S - NEB) in the way that the protein is secreted into the medium. I know that I can get some protein but it is not active. As ...
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Computer Models of E Coli / Saccharomyces cerevisiae metabolite pathways
Are there mathematical models one can use to predict the expression levels of a foreign gene inserted into a well studied host organism like E Coli or S cerevisiae?
I'm not, obviously, looking for ...
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Speed of cell signal to protein production
How long does a cell take to make a protein from the point of receiving the signal to produce the protein? Does the cell have stores of the protein ready to release or is the protein created in real-...
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What is the optimal pO2 concentration for automated reactor batch mode EColi expression?
I am wondering what is the optimal pO2 level for an reactor based expression? All protocols I have found indicate for E.Coli pO2 levels just bigger 20%. So, I am wondering what is the optimal level? ...
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What is a good expression vector for Salmonella enterica subsp. enterica serovar typhimurium
I am interested in expressing custom proteins in a Salmonella strain, however I am facing difficulties in finding the appropriate expression vector for it. It seems that most of the resources provide ...
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Why there are two types of medium (YPGlu and YPGal) in the *K. lactis* Protein Expression Kit?
I am planning to use the K. lactis Protein Expression Kit from NEB for the expression of certain protein. I am not experienced with working with yeast. I started by reading the manual for this kit and ...
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Eukaryotic cell lysate-based protein expression efficiency
What is average efficiency of eukaryotic cell lysate-based protein expression systems in terms of (mg of protein expressed) / (mg of lysate) ?
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Cloning using pET28a and Protein Expression in DH5alpha and BL21
Can someone please direct me to an e-resource or a book that will help a newbie like me learn in depth about Cloning using pET28a and Protein Expression in DH5alpha and BL21. Though I have done ...