The protein-folding tag has no wiki summary.
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1answer
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How difficult is to renature a protein?
I know of Anfinsen's experiments and I'm aware that some denatured enzymes may regain their lost activity through the removal of the denaturant agent. What I'm unaware of is how rare is it for a ...
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2answers
64 views
Differences Between Protein Motifs and Protein Domains?
I am in a 300-level molecular biology class and am unclear about this concept and how to delineate motifs versus domains of proteins. Any suggestions would be much obliged.
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How to wash the column during protein purification with GST tag?
I have been working with GST tagged proteins for the last 4 years and after loading the cell lysate into the column I was washing it with 20-30 column volumes of PBS and sometimes my proteins were ...
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3answers
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A question regarding evolution
I'm not a biologist so bear with me. I know that the DNA molecule carries extremely large amounts of information. If the process of evolution is driven by completely random process .I think about it ...
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1answer
25 views
position of cell penetrating peptide
I'd like to add a cell penetrating peptide to my custom peptide (30aa). Can I just add it to the end of the peptide sequence or does it have to be positioned on an outward facing external chain?
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0answers
40 views
Experimental techniques to measure protein-protein and protein-ligand structure and dynamics
We would like to compare our simulation results with experimentation. When one wants to follow experimentally the dynamics (and possibly structure) of protein-protein or protein-ligand docking ...
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2answers
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What are some of the immediate challenges to break through before finding a cure for mad cow disease?
What are the immediate challenges to break through in seeking a cure for mad cow disease?
I know that mad cow disease has no treatment as of yet.
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0answers
73 views
Is whey-protein folding an example of micelle in water? If not, what is it?
I can find a lot of information about protein folding in water but not about whey-protein-folding in water that body-builders use with filtered shakers. Without filters, you get protein balls -- is ...
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2answers
331 views
What are the most important differences between HSP70 and HSP90?
Question originally asked on Quora. These proteins have many functional similarities, so why do cells need both to handle unfolded proteins?
