The process in which a protein folds into a three-dimensional shape to achieve proper functioning.

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1answer
67 views

What causes a polypeptide to fold into an Alpha helix over a Beta pleated sheet

I know how they fold, but what causes some polypeptide chains to preferably fold into Alpha-helix rather than Beta-sheets (or vice-versa). What force makes it fold into one conformation over the ...
1
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1answer
183 views

Is whey-protein folding an example of micelle in water? If not, what is it? [closed]

I can find a lot of information about protein folding in water but not about whey-protein-folding in water that body-builders use with filtered shakers. Without filters, you get protein balls -- is ...
3
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1answer
34 views

Verifying Protein Folds

I have recently begun using Folding@Home and I am curious how people are prevented from cheating the system. It seems to me that unless the final result is easily verifiable users could submit bogus ...
3
votes
1answer
121 views

How to wash the column during protein purification with GST tag?

I have been working with GST tagged proteins for the last 4 years and after loading the cell lysate into the column I was washing it with 20-30 column volumes of PBS and sometimes my proteins were ...
1
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0answers
32 views

Planar circular DNA and protein [closed]

I am trying to develop a planar (two dimensional) lattice based framework to simulate ring (circular) DNA and proteins. To verify my model, I need to simulate real DNA and proteins. The base idea for ...
3
votes
1answer
349 views

Is there an optimal composition and length for protein linkers in FRET?

I'm designing a protein that I'd like to use in FRET reporting. General idea on the shape is: FRETprotein1--Linker--CleavageSite--Linker--FRETprotein2. I would like to know what AA are best for the ...
3
votes
1answer
84 views

Which of Perutz et al's protein structures other than 3₁₀ helix were correct?

In 1950, Bragg, Kendrew and Perutz published "Polypeptide chain configurations in crystalline proteins" (open access) and were famously 'proved wrong' by Pauling, Corey and Branson the following year, ...
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3answers
12k views

Differences Between Protein Motifs and Protein Domains?

I am in a 300-level molecular biology class and am unclear about this concept and how to delineate motifs versus domains of proteins. Any suggestions would be much obliged.
1
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1answer
106 views

Which is the correct statement on proteins?

I need help with one of the questions on my biochemistry assignment Choose the correct statement on proteins: a) proteins are folded by alpha-helix b) proteins can preserve their function even if ...
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1answer
49 views

Function of ER in reviewing mutated proteins

At least in the case of Cystic Fibrosis it happens that a mutant protein (which could actually function!) is held in the ER because the ER detects it as misfolded. Does this happen in every type of ...
1
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1answer
90 views

Statistical Analysis of Protein Folding Problem [closed]

I’m new to the field of protein folding. I’ve been searching and came across some books for predicting structures (Introduction to Protein Structure Prediction: Methods and Algorithms). Does anyone ...
1
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1answer
2k views

How difficult is to renature a protein?

I know of Anfinsen's experiments and I'm aware that some denatured enzymes may regain their lost activity through the removal of the denaturant agent. What I'm unaware of is how rare is it for a ...
4
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2answers
74 views

What are some of the immediate challenges to break through before finding a cure for mad cow disease?

What are the immediate challenges to break through in seeking a cure for mad cow disease? I know that mad cow disease has no treatment as of yet.
4
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1answer
41 views

position of cell penetrating peptide

I'd like to add a cell penetrating peptide to my custom peptide (30aa). Can I just add it to the end of the peptide sequence or does it have to be positioned on an outward facing external chain?
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3answers
151 views

A question regarding evolution

I'm not a biologist so bear with me. I know that the DNA molecule carries extremely large amounts of information. If the process of evolution is driven by completely random process .I think about it ...
3
votes
0answers
73 views

Experimental techniques to measure protein-protein and protein-ligand structure and dynamics

We would like to compare our simulation results with experimentation. When one wants to follow experimentally the dynamics (and possibly structure) of protein-protein or protein-ligand docking ...
2
votes
2answers
854 views

What are the most important differences between HSP70 and HSP90?

Question originally asked on Quora. These proteins have many functional similarities, so why do cells need both to handle unfolded proteins?