Tagged Questions
1
vote
1answer
39 views
How difficult is to renature a protein?
I know of Anfinsen's experiments and I'm aware that some denatured enzymes may regain their lost activity through the removal of the denaturant agent. What I'm unaware of is how rare is it for a ...
4
votes
2answers
71 views
Differences Between Protein Motifs and Protein Domains?
I am in a 300-level molecular biology class and am unclear about this concept and how to delineate motifs versus domains of proteins. Any suggestions would be much obliged.
1
vote
0answers
27 views
How to wash the column during protein purification with GST tag?
I have been working with GST tagged proteins for the last 4 years and after loading the cell lysate into the column I was washing it with 20-30 column volumes of PBS and sometimes my proteins were ...
4
votes
1answer
25 views
position of cell penetrating peptide
I'd like to add a cell penetrating peptide to my custom peptide (30aa). Can I just add it to the end of the peptide sequence or does it have to be positioned on an outward facing external chain?
2
votes
2answers
340 views
What are the most important differences between HSP70 and HSP90?
Question originally asked on Quora. These proteins have many functional similarities, so why do cells need both to handle unfolded proteins?
