Polypeptide chains of amino acids folded into shape by charged R groups
9
votes
1answer
88 views
Protein data bank syntax: Can two protein secondary structures “overlap”?
I have a technical question regarding the syntax in Protein Data Bank files. In the protein with PDB# 1AE9 (pdb file), there are two lines in the .pdb file:
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1
vote
1answer
27 views
How difficult is to renature a protein?
I know of Anfinsen's experiments and I'm aware that some denatured enzymes may regain their lost activity through the removal of the denaturant agent. What I'm unaware of is how rare is it for a ...
7
votes
0answers
193 views
Rosetta ab initio prediction and protein-protein interaction fitness help
I have designed several proteins which I predict have interactions with another protein using the sequence based Conjoint Triad Method. I would like to know which ones structurally are predicted to ...
2
votes
0answers
32 views
Where to put the gene after eukaryotic promoter for best expression levels?
As far as I know there is an optimum distance between a promoter and the gene for the best expression levels. What is that distance for common promoters like CMV, SV40? If you have a first hand ...
2
votes
0answers
25 views
Endocannabinoid Transporters - which ones are Likely to Exist and what is the Evidence for their Respective Existence?
Endocananbinoid = Endogenous Cannabinoid or CB1 and CB2 ligands.
I'm asking which endocannabinoid transporters exist and could the one answering show all the evidence for the existence of the ...
2
votes
0answers
152 views
What specifically allows alpha-complementation in beta-galactosidase?
I'm familiar with the use of alpha-complementation of beta-galactosidase with the pUC alpha-peptide and the M15 lacZ gene product, and would like to hypothetically apply alpha-complementation in other ...
2
votes
0answers
44 views
Are there examples of PTMs that add different chemical groups (and mass) to different amino acids?
I'm talking about this type of post-translational modifications. What I'm interested in is not whether some modifications can only occur on specific amino acids (that's clear), but if the nomenclature ...
1
vote
0answers
11 views
How to use mechanical microstrainer to extract tissue proteins from human?
Background:
There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
1
vote
0answers
19 views
What is the translation termination efficiency in mammalian cells?
When I express proteins in bacteria I put at least two stop codons at the end of the gene to increase the termination efficiency. Is this the case in eukaryotic cells too? If I put a single stop codon ...
1
vote
0answers
26 views
How to wash the column during protein purification with GST tag?
I have been working with GST tagged proteins for the last 4 years and after loading the cell lysate into the column I was washing it with 20-30 column volumes of PBS and sometimes my proteins were ...
0
votes
0answers
15 views
activate a drug using deoxycytidine kinase in vitro
I need to activate a drug similar to the way it is activated in the cell. My set of molecules called Nucleoside analog reverse-transcriptase inhibitors (NARTIs or NRTIs) need three phosphate groups to ...
0
votes
0answers
98 views
Difference between Condensin and Cohesin proteins?
The chromosomal DNA is stacked with help of cohesin and condensin protein in which particular manner? Can cohesin be said to form kinetochore? How would they vary exactly?
The terms are so narrowly ...
