3
votes
1answer
77 views

Appropriate Buffer for electerophoresis of DNA & Protein TBE or TAE? [on hold]

Which buffer is best for DNA Electrophoresis and which is best for Protein to be have a sharp bond? Considering a higher electrical conductivity compared to TAE & TBE and the generation of less ...
4
votes
2answers
688 views

Why is proteinase K digestion performed at 50 °C?

Many DNA isolation protocols use a Proteinase K digestion to remove proteins. This is often performed at 50 °C. Why is this?