to customize your list.
more stack exchange communities
Start here for a quick overview of the site
Detailed answers to any questions you might have
Discuss the workings and policies of this site
Ligase Chain Reaction: Determining the annealing temperature for gene synthesis
Using the tool Gene2Oligo I have a set of DNA oligonucleotides to synthesize a gene using ligase chain reaction (LCR). The average melting temperature is 72 degrees Celsius. I am going to use a the ...
May 29 '13 at 2:37
newest protocol lcr questions feed
Hot Network Questions
What limits the size of digital imaging sensors?
Python code for detecting a single item moved in a list
What do 'hitbox' and 'hurtbox' mean?
Does car tire pressure change with weight of car load?
How to remove a line of a matrix if not all elements are numeric?
Open and closed doors riddle
Pedagogical exercise - How to synthesize methamphetamine
Commutator algebra in exponents
Minimisation problem in thousands of dimensions
iPad stolen. Can we retrieve a Minecraft world?
Why does everyone face the same way on the transporter?
Poker in the Star Trek universe : What are the stakes?
Should I call my adviser by her first name?
Can I use garlic leaf for cooking?
In the word "Scent", is the S or the C silent?
What is the name of the mineral that can be found before Mithril in Moria?
scissors cut/cuts paper, which is correct?
How can I get hiring managers to take cost of living into account when they ask for my current salary?
Why are we so careful in saying that dy/dx is not a fraction?
Raster diff: how to check if images have identical values?
Transit through Beijing - Languages?
How do I make it absolutely clear that I'm shooting you in the face?
What are some effective ways to deal with sand when camping on or near the beach?
Is it possible for an SU-25 to fly high enough to shoot down a Boeing 777?
more hot questions
Life / Arts
Culture / Recreation
TeX - LaTeX
Unix & Linux
Ask Different (Apple)
Geographic Information Systems
Science Fiction & Fantasy
Seasoned Advice (cooking)
Personal Finance & Money
English Language & Usage
Mi Yodeya (Judaism)
Cross Validated (stats)
Theoretical Computer Science
Meta Stack Exchange
Stack Overflow Careers
site design / logo © 2014 stack exchange inc; user contributions licensed under
cc by-sa 3.0