A predetermined methodology for carrying out an experiment.

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4
votes
2answers
977 views

Why is proteinase K digestion performed at 50 °C?

Many DNA isolation protocols use a Proteinase K digestion to remove proteins. This is often performed at 50 °C. Why is this?
2
votes
0answers
125 views

Has anybody used Evrogen's DSN-normalization protocol for cDNA normalization? [closed]

I found the link to a commercial product by Evrogen to normalise cDNA samples for gene discovery projects here: http://www.evrogen.com/technologies/normalization.shtml The most up-to-date reference ...
13
votes
1answer
235 views

How straightforward is in vitro compartmentalization?

in vitro compartmentalization (IVC) is one of those technologies that everyone knows about, talks about, but never actual does due to the rather technical difficulties in setting the system up. I was ...
11
votes
2answers
208 views

protocol for pulldown of DNA breakpoints?

Is there any method to do pulldown enrichment of DNA breakpoints from a cell? I have found this paper reporting a method to enrich for the DNA single-strand breakpoints from meiotic recombination ...
5
votes
2answers
2k views

How to set up a slow cooling on an AB Veriti thermal cycler?

I want to incubate my sample at 50ºC and then slowly cool it down to 4ºC at a rate of 0.1ºC/s. I am using AB Veriti thermal cycler. Does anyone know how to set up the rate of cooling? It has to do ...