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2
votes
1answer
42 views
What is the point of being selection-free?
I'm reading "Highly efficient endogenous human gene correction using designed zinc finger nucleases" by Urnov et al. They propose a way to use zinc finger proteins for gene therapy. They repeatedly ...
6
votes
1answer
75 views
Proteins that are strongly overproduced in E. coli and S. cerevisiae?
I'm looking for some pointers to proteins that produce at really gigantic levels in E. coli and yeast (S. cerevisiae). Can anyone point to some champion proteins?
Even in inclusion bodies and non ...
5
votes
1answer
67 views
Recombinant protein fraction in E. coli
If a protein is heterologously expressed in E. coli under the T7 promoter, what fraction of the total protein concentration in the cell is the heterologously expressed protein? What could be its ...
