Microbially (usually bacterial) derived endonucleases which cleave DNA at defined "restriction" sites
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How prevalent is Taq polymerase in adding 3' A overhangs to the PCR product?
I am conducting a mutagenesis on a gene in vivo of which I need to ligate into an expression vector. The primers I have designed overlap restriction sites of which I plan to use to ligate into the ...
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vote
2answers
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Restriction Mapping of Plasmid Assignment
pUWL22 is a circular, double stranded, 10.5 kb plasmid. It contains a
gene encoding an enzyme that confers ampicillin resistance in the host
bacterium. Cloning into the kpn I and Sst I sites ...
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votes
2answers
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Primer design for introduction of restriction sites flanking a gene of interest
I am wondering what the correct method for primer design to introduce restriction sites. Specifically between two methods.
1) Primer first partially hybridises to the gene, has a mis-match where the ...
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votes
3answers
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Why do restriction enzymes tend to have an even number of bases in their recognition site?
When reading my textbook I noticed that in all examples but one from eight the recognition site was an even number of bases.
I wondered if this was just a co-incidence, so I took the data from this ...
