Retroviruses are single-stranded RNA viruses that reverse-transcribe their genome into DNA that is then integrated into the host genome.

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Why is HIV so large compared to other viruses?

The HIV-1 virus is about 120-150 nm in size and has a genome only about 10,000 base pairs long. Other viruses are far more efficient, for example lambda phage has something like a tenth the volume ...
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Transmission of active exogenous retrovirus

Are there any known examples of retroviruses becoming fixed in the genome of an organism (ie infecting the germ line), but remaining infective through horizontal transmission? Is there usually a ...
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Why does HIV belong to a group of retroviruses called lentiviruses?

HIV is an enveloped retrovirus. Each virus particle contains two copies of an RNA genome. The virus also has a number of enzymes: reverse transcriptase, integrase and viral protease. But, once I also ...
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Human Endogenous Retroviruses

I am reading this paper, which shows that a Human Endogenous Retrovirus (HERV) K provirus is present at the orthologous position of gorilla and chimpanzee genomes but absent in the human genome. If ...
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Transmission of HIV from Mother-to-Child Through Breastfeeding

If a mother is HIV+ and was on medications which would help her deliver a HIV negative baby, can the baby contract the HIV through her breast milk?
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Recommendations for an intro level virology textbook? [closed]

I'm a college sophomore, and I was just accepted into a research lab that works with retroviruses. Since I haven't taken any classes on the topic yet, does anyone have recommendations for good, ...
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Retrovirus Production

I have been having difficulties with low transduction efficiencies of my retrovirus production. I expand my plasmid of interest (on MiG-GFP plasmid) in DH5α E Coli for ~24 hours, purify with Qiagen ...
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How to isolate host cell RNA (tRNA,mRNA,rRNA) from viral RNA?

A retrovirus with RNA genome infected a host cell. You would like to isolate the host cell’s RNA (rRNA, tRNA, and mRNA) from the virus RNA. What properties can you rely on to determine the three types ...
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Why AZT is selective towards HIV and doesn't impair human DNA replication?

I've found this article, which is a very old one (from the time when nucleoside analogs where researched as a possible way to prevent replication of virus genetic material, before the HIV epidemics). ...
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How does HIV mutate into other strains while keeping their virulent phenotype?

How does a virus like HIV mutate into so many strains, and yet all of them are harmful to our immune system? What gives this virus the ability to mutate so efficiently?
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Are some virus loads introduced to human cell but never triggered?

Is there a term or any evidence of phage DNA integrating into chromosomes/ DNA but never being triggered? For example, could a virus that affected Neanderthals still infect human cells today but is ...
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Are retroviruses cytotoxic?

There are typically hundreds of retroviruses found in healthy human beings. Are retroviruses then cytotoxic? (In other words, are they able to kill or damage other cells).
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Is there an 'anti-virus'?

A virus spreads around and usually attaches itself to the host, multiplies & causes diseases. But is there something like an anti-virus? A single celled entity that does the opposite: spreads ...
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Is it impossible for a retrovirus to be lysogenic?

Is it impossible for retroviruses to be lysogenic? In the lysogenic cycle, the viral genetic material is incorporated into the host cell's DNA. Because retroviruses have RNA, it would be impossible ...
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Mutation rate in viruses

Mutation rate is a phenotypic trait that evolves. The process of evolution of such kind of traits are often referred to as evolvability. I am wondering about the evolution of the mutation rates in ...
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169 views

Why use DNA polymerase in making cDNA?

RT is capable of synthesizing a complementary dna strand ( as in HIV life cycle.) Then why is DNA pol used when cDNA (synthesizing the second strand of it ) has to be synthesized from mRNA ( For eg.to ...
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How is RNA of retrovirus converted into cDNA?

The retrovirus (oncovirus) contains RNA. It also has a molecule called reverse transcriptase. This molecule transcribes RNA into cDNA. This cDNA is the DNA copy of viral RNA genome RNA has Uracil ...
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Can retroviral delivery systems “overwrite” genes?

As the question states, what are the limits of retroviral genetic delivery systems? Are they limited to adding additional gene sequences to a cell, or can they actually overwrite specified segments ...