Organelles responsible for protein synthesis in cells. They are distinguished into "free" and "membrane-bound" classes.
6
votes
1answer
72 views
How was the first ribosome theorized to have been made?
Seeing as ribosomes are what help perform the act of translation itself, and the blueprints for they themselves are in nucleotide sequences, how was the first ribosome theorized to have been made? ...
11
votes
1answer
126 views
Can DNA act as a translation substrate?
I get conflicting answers. One would think if it was true, it would be rather seminal and widely known.
There are papers from Khorana[1], Holland[2], and Bretscher[3] (late 60s) that suggest that it ...
7
votes
1answer
50 views
Sequence of ribosomal RNA
Is it possible to sequence rRNA directly, that is, using the ribosome rather than the DNA from the nucleus? For example, this paper, Complete nucleotide sequence of a 16s rRNA gene from E. coli, ...
4
votes
1answer
93 views
Is it possible to sequence only the 16S rRNA component? If so, how?
There have recently been several papers on using 16S rRNA as a way of identifying species (here, and here). I'm wondering if it's possible to sequence either just that subunit of the ribosome or just ...
5
votes
4answers
1k views
RNA or ribosome, which one moves during translation?
During translation ribosomes decode the genetic information present in the mRNA and protein synthesis takes place. During this process which of those two does move, the ribosome or the mRNA?
4
votes
0answers
38 views
What is the distance between the 3' 18s rRNA (the Kozak consensus sequence) and the A-site of eukaryotic ribosomes during protein translation?
Reposted from Quora http://www.quora.com/What-is-the-distance-between-the-3-18s-rRNA-the-Kozak-consensus-sequence-and-the-A-site-of-eukaryotic-ribosomes-during-protein-translation
The Kozak sequence ...
6
votes
1answer
172 views
How do ribosomes contribute to their own synthesis?
In other words, what products synthesized by ribosomes are actual parts of ribosomes (if any)? How are they involved in their own synthesis otherwise? What is the cycle/chain of products starting ...
16
votes
1answer
1k views
What is the criticality of the ribosome binding site relative to the start codon in prokaryotic translation?
In prokaryotic translation, how critical for efficient translation is the location of the ribosome binding site, relative to the start codon?
Ideally, it is supposed to be -7b away from the start. ...
